Direct and indirect effects of silver nanoparticles on freshwater and marine microalgae (Chlamydomonas reinhardtii and Phaeodactylum tricornutum)

Sendra, Marta; Yeste, María Pilar; Gatica, José M.; Moreno-Garrido, Ignácio; Blasco, Julián

doi:10.1016/j.chemosphere.2017.03.123

Cited by 112 publications

(66 citation statements)

References 48 publications

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“…1). The results are comparable with other data on behaviour of different types of AgNPs in ASW (Sendra et al, 2017, Schiavo et al, 2017. No differences in ζ-potential were observed in ASW and HS, with values close to the point of zero charge (-1.5 ± 3.7 mV).…”

Section: Effects On Hemocytessupporting

confidence: 90%

“…Overall, the results indicate that in our experimental conditions mitochondria and cytoskeletal structures represent the main targets of AgNPs; however, these data do not indicate a toxicity scenario mediated by Ag + release. Indeed, on the basis of extensive literature data, in our experimental conditions AgNP should not release Ag + in sufficient quantities to contribute to the overall effects observed in short time exposure experiments (30 min) (Sendra et al, 2017;Schiavo et al, 2017;Liu and Hurt, 2010;Levard et al, 2012). This hypothesis is reinforced by the large gap in effective concentrations and type of effect between the nanoparticulate and soluble Ag + forms.…”

Section: Effects On Hemocytesmentioning

confidence: 82%

“…According to Gottschalk et al (2009) Predicted Environmental Concentrations (PECs) of AgNP for surface water in Europe are expected to be in the low ng/Lrange (0.76 ng/L), but it is expected to be released in larger quantities within the next decades (Fabrega et al, 2011;McGillicuddy et al, 2017). Once in the aquatic environment, AgNPs can undergo several transformation processes (agglomeration/aggregation, oxidation, dissolution, adsorption with soluble and particulate organic matter); in particular, release of Ag + ions may represent an additional source of silver in the environment (Levard et al, 2012;Sendra et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Effects of nanosilver on Mytilus galloprovincialis hemocytes and early embryo development

et al. 2018

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Silver nanoparticles (AgNP), one of the main nanomaterials for production and use, are expected to reach the aquatic environment, representing a potential threat to aquatic organisms. In this study, the effects of bare AgNPs (47 nm) on the marine mussel Mytilus galloprovincialis were evaluated at the cellular and whole organism level utilizing both immune cells (hemocytes) and developing embryos. The effects were compared with those of ionic Ag(AgNO). In vitro short-term exposure (30 min) of hemocytes to AgNPs induced small lysosomal membrane destabilization (LMS EC = 273.1 μg/mL) and did not affect other immune parameters (phagocytosis and ROS production). Responses were little affected by hemolymph serum (HS) as exposure medium in comparison to ASW. However, AgNPs significantly affected mitochondrial membrane potential and actin cytoskeleton at lower concentrations. AgNO showed much higher toxicity, with an EC = 1.23 μg/mL for LMS, decreased phagocytosis and induced mitochondrial and cytoskeletal damage at similar concentrations. Both AgNPs and AgNO significantly affected Mytilus embryo development, with EC = 23.7 and 1 μg/L, respectively. AgNPs caused malformations and developmental delay, but no mortality, whereas AgNO mainly induced shell malformations followed by developmental arrest or death. Overall, the results indicate little toxicity of AgNPs compared with AgNO; moreover, the mechanisms of action of AgNP appeared to be distinct from those of Ag. The results indicate little contribution of released Ag in our experimental conditions. These data provide a further insight into potential impact of AgNPs in marine invertebrates.

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Section: Effects On Hemocytessupporting

confidence: 90%

Section: Effects On Hemocytesmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

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Effects of nanosilver on Mytilus galloprovincialis hemocytes and early embryo development

et al. 2018

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“…In addition, AgNPs toxicity may depend on the species and on the type of growth medium in which the organisms are cultivated [44]. However, some adverse effects can also be attributed to speci c properties of NPs, such as the size and the degree of aggregation, that in seawater is increased when compared to freshwater [45,46], and that in turn affect the capacity of NPs to cross biological membrane or to bind the cell surface [47,48]. Cell wall, in fact, constitutes a primary site for interaction and serves as a barrier for the entrance of AgNPs into algal cells.…”

Section: Discussionmentioning

confidence: 99%

“…Once the cell wall is penetrated, endocytic passage through plasma membrane may be possible and internalised NPs enhance biological effects. Sendra et al [46] found that the attachment of Ag NPs on the surfaces of freshwater and marine microalgae Chlamydomonas reinhardtii and Phaeodactylum tricornutum, and the presence of AgNPs inside cells directly drives the toxic effects. NPs can also enter into the cells via ion channels, transport proteins and endocytosis mechanism [50].…”

Section: Discussionmentioning

confidence: 99%

Toxicity, bioaccumulation and biotransformation of glucose capped silver nanoparticles in green microalgae Chlorella vulgaris

Mariano

Panzarini

Inverno

et al. 2020

Preprint

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BackgroundSilver nanoparticles (AgNPs) are one of the most widely used nanomaterials in consumer products. When discharged into the aquatic environment AgNPs can cause toxicity to aquatic biota, through mechanisms that are still under debate, thus rendering the NPs effects evaluation a necessary step. Different aquatic organism models, i.e. microalgae, mussels, Daphnia magna, sea urchins and Danio rerio, etc. have been largely exploited for NPs toxicity assessment. On the other hand, alternative biological microorganisms abundantly present in nature, i.e. microalgae, are nowadays exploited as a potential sink for removal of toxic substances from the environment. Indeed, the green microalgae Chlorella vulgaris is one of the most used microorganisms for waste treatment.ResultsWith the aim to verify the possible involvement of C. vulgaris not only as a model microorganism of NPs toxicity but also for the protection toward NPs pollution, we used these microalgae to measure the AgNPs biotoxicity and bioaccumulation. In particular, to exclude any toxicity derived by Ag+ ions release, green chemistry synthesised and Glucose coated AgNPs (AgNPs-G) were used. C. vulgaris actively internalised AgNPs-G whose amount increases in a time and dose-dependent manner. The internalised NPs, found inside large vacuoles, were not released back into the medium, even after 1 week, and did not undergo biotransformation since AgNPs-G maintained their crystalline nature. Biotoxicity of AgNPs-G causes an exposure time and AgNPs-G dose-dependent growth reduction and a decrease in chlorophyll-a amount.ConclusionsThese results confirm C. vulgaris as a biomonitoring organism and also suggest it as a bioaccumulating microalgae for possible use in the environment protection.

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Silver Nanoparticles as Stimulators in Biotechnology of Porphyridium cruentum

et al. 2022

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Direct and indirect effects of silver nanoparticles on freshwater and marine microalgae (Chlamydomonas reinhardtii and Phaeodactylum tricornutum)

Cited by 112 publications

References 48 publications

Effects of nanosilver on Mytilus galloprovincialis hemocytes and early embryo development

Effects of nanosilver on Mytilus galloprovincialis hemocytes and early embryo development

Toxicity, bioaccumulation and biotransformation of glucose capped silver nanoparticles in green microalgae Chlorella vulgaris

Silver Nanoparticles as Stimulators in Biotechnology of Porphyridium cruentum

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