Direct Analysis of Biological Tissue by Paper Spray Mass Spectrometry

Wang, He; Manicke, Nicholas E.; Yang, Qian; Zheng, Lingxing; Shi, Riyi; Cooks, R. Graham; Ouyang, Zheng

doi:10.1021/ac103150a

Cited by 222 publications

(181 citation statements)

References 48 publications

(76 reference statements)

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“…Some noncapillary tips such as stainless steel needle [6,19], paper [13,[20][21][22][23], and wooden tips [14] enabled direct analysis of raw samples. Hiraoka and co-workers reported the sequential and exhaustive ionization of analytes on solid metal probe [24].…”

Section: E Lectrospray Ionization (Esi) Is a Powerful Massmentioning

confidence: 99%

Analytical Properties of Solid-substrate Electrospray Ionization Mass Spectrometry

Yao

2012

J. Am. Soc. Mass Spectrom.

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Abstract. Conventional electrospray ionization mass spectrometry (ESI-MS) uses a capillary for sample loading and ionization. Along with the development of ambient ionization techniques, ESI-MS using noncapillary emitters has attracted more interest in recent years. Following our recent report on ESI-MS using wooden tips (Anal. Chem. 83, 8201-8207 (2011)), the technique was further investigated and extended in this study. Our results revealed that the wooden tips could serve as a chromatographic column for separation of sample components. Sequential and exhaustive ionization was observed for proteins and salts on wooden tips with salts ionized sooner and proteins later. Nonconductive materials that contain microchannels/pores could be used as tips for ESI-MS analysis with sample solutions loaded to the sharp-ends only, since rapid diffusion of sample solutions by capillary action would enable the tips to become conductive. Tips of inert materials such as bamboo, fabrics, and sponge could be used for sample loading and ionization, while samples such as tissue, mushroom, and bone could form tips to induce ionization for direct analysis with application of a high voltage.

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Section: E Lectrospray Ionization (Esi) Is a Powerful Massmentioning

confidence: 99%

Analytical Properties of Solid-substrate Electrospray Ionization Mass Spectrometry

Yao

2012

J. Am. Soc. Mass Spectrom.

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“…Other applications include analysis of agrochemicals and contaminants in food [15][16][17], tissue analysis [18], surface swabbing [19,20], profiling of algae [21] and bacteria [22,23], cell culture monitoring [24], and herbicides [25] in water and crop extracts. In addition to applications development, methodological advancements have been reported for paper spray MS such as improving selectivity using differential mobility spectrometry prior to MS detection [26], improving sensitivity using solid phase extraction [27], the use of alternative porous substrates [7,[28][29][30], and using paper spray as an ionization source for microfluidics chips [31,32].…”

mentioning

confidence: 99%

Ionization Suppression and Recovery in Direct Biofluid Analysis Using Paper Spray Mass Spectrometry

Vega

Spence

Zhang

et al. 2016

J. Am. Soc. Mass Spectrom.

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Abstract. Paper spray mass spectrometry is a method for the direct analysis of biofluid samples in which extraction of analytes from dried biofluid spots and electrospray ionization occur from the paper on which the dried sample is stored. We examined matrix effects in the analysis of small molecule drugs from urine, plasma, and whole blood. The general method was to spike stable isotope labeled analogs of each analyte into the spray solvent, while the analyte itself was in the dried biofluid. Intensity of the labeled analog is proportional to ionization efficiency, whereas the ratio of the analyte intensity to the labeled analog in the spray solvent is proportional to recovery. Ion suppression and recovery were found to be compound-and matrix-dependent. Highest levels of ion suppression were obtained for poor ionizers (e.g., analytes lacking basic aliphatic amine groups) in urine and approached -90%. Ion suppression was much lower or even absent for good ionizers (analytes with aliphatic amines) in dried blood spots. Recovery was generally highest in urine and lowest in blood. We also examined the effect of two experimental parameters on ion suppression and recovery: the spray solvent and the sample position (how far away from the paper tip the dried sample was spotted). Finally, the change in ion suppression and analyte elution as a function of time was examined by carrying out a paper spray analysis of dried plasma spots for 5 min by continually replenishing the spray solvent.

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“…is technique was demonstrated to be able to not only analyze complex samples, but also perform quantitative analysis. 29,30,[32][33][34][35] Paper spray was successfully applied in direct detection and quantitation of therapeutic drugs in blood and tissue samples, providing a rapid and reliable method in therapeutic monitoring. 29,30,[32][33][34] Very recently, this technique was utilized in determination of drugs-ofabuse in biological uids 35) and trace amount of illicit drugs on a solid surface (desktop).…”

Section: Paper Spraymentioning

confidence: 99%

“…29,30,[32][33][34][35] Paper spray was successfully applied in direct detection and quantitation of therapeutic drugs in blood and tissue samples, providing a rapid and reliable method in therapeutic monitoring. 29,30,[32][33][34] Very recently, this technique was utilized in determination of drugs-ofabuse in biological uids 35) and trace amount of illicit drugs on a solid surface (desktop). 30) Furthermore, the use of paper spray in food analysis, e.g., determination of Sudan azo-dyes in chilli pepper, 36) detection of agrochemicals on fruit peels, 30) chemical ngerprint analysis of Bansha herbal tea 37) and determination of nonosteroidal anti-in ammatory drugs in olive oil, 38) were demonstrated.…”

Section: Paper Spraymentioning

confidence: 99%

Electrospray Ionization on Solid Substrates

Yao

2014

Mass Spectrometry

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Development of electrospray ionization on solid substrates (solid-substrate ESI) avoids the clogging problem encountered in conventional capillary-based ESI, allows more convenient sampling and permits new applications. So far, solid-substrate ESI with various materials, e.g., metals, paper, wood, bers and biological tissue, has been developed, and applications ranging from analysis of pure compounds to complex mixtures as well as in vivo study were demonstrated. Particularly, the capability of solid-substrate ESI in direct analysis of complex samples, e.g., biological uids and foods, has signi cantly facilitated mass spectrometric analysis in real-life applications and led to increasingly important roles of these techniques nowadays. In this review, various solid-substrate ESI techniques and their applications are summarized and the prospects in this eld are discussed.Please cite this article as: P.-K. So, et al., Electrospray Ionization on Solid Substrates, Mass Spectrom (Tokyo) 2014; 3(2): S0028;

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Direct Analysis of Biological Tissue by Paper Spray Mass Spectrometry

Cited by 222 publications

References 48 publications

Analytical Properties of Solid-substrate Electrospray Ionization Mass Spectrometry

Analytical Properties of Solid-substrate Electrospray Ionization Mass Spectrometry

Ionization Suppression and Recovery in Direct Biofluid Analysis Using Paper Spray Mass Spectrometry

Electrospray Ionization on Solid Substrates

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