Dihydroartemisinin and Curcumin Synergistically Induce Apoptosis in SKOV3 Cells Via Upregulation of MiR-124 Targeting Midkine

Zhao, Jiaojiao; Pan, Yuchen; Li, Xiujun; Zhang, Xuefang; Yuan, Xue; Wang, Tingting; Zhao, Shuli; Hou, Yayi

doi:10.1159/000480531

Cited by 35 publications

(24 citation statements)

References 35 publications

(28 reference statements)

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“…The data demonstrated that DHA synergizes with rapamycin and promotes cell death and apoptosis via the upregulation of DAPK and Atg7, which provides an indication for its usefulness in anticancer studies and clinical application. In accordance with the results of other studies regarding DHA, the effects of DHA on autophagy were further confirmed (28)(29)(30)(31)(32). The present study provides novel evidence regarding the effects of rapamycin combined with DHA and Atg7 knockdown; however, owing to the limitations of in vitro experiments, the associated combined effects should be investigated in in vivo animal models in the future, with further cell lines investigated.…”

Section: Discussionsupporting

confidence: 90%

“…Zhao et al (32) demonstrated that combined treatment with DHA and curcumin decreased cell viability, arrested the cell cycle and promoted apoptosis in human ovarian cancer SKOV3 cells. It was also identified that treatment with DHA alone has the ability to induce limited apoptosis, arrest the cell cycle at the S and G 2 /M phases and inhibit tumor growth in a xenograft model established by subcutaneously injecting SKOV3 cells into the right flank of female BALB/c nude mice, without notable toxicity (32). Additionally, caspase-3 was not activated following treatment with DHA, and caspase-3 inhibitors did not inhibit cell apoptosis in any group; therefore, it was hypothesized that DHA may induce cell apoptosis via other apoptotic pathways (32).…”

Section: Discussionmentioning

confidence: 99%

“…Consistent with these previous studies, the present study identified that DHA induced autophagy. Despite the presence of numerous studies (28)(29)(30)(31)(32), there is limited knowledge regarding the effect of autophagy on the anticancer action of DHA. The present study revealed the role of autophagy in the anticancer action of DHA within MDA-MB-231 cells.…”

Section: Discussionmentioning

confidence: 99%

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Rapamycin promotes the anticancer action of dihydroartemisinin in breast cancer MDA-MB-231 cells by regulating expression of Atg7 and DAPK

Liu

Zhou

et al. 2018

Oncol Lett

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Abstract. There is limited knowledge regarding the influence of autophagy on the anticancer effect of dihydroartemisinin (DHA). The present study aimed to investigate this influence within human breast cancer cells. Changes in cell viability, cell cycle distribution, apoptosis and associated genes were analyzed in MDA-MB-231 cells subjected to DHA following alteration in autophagy levels; the autophagy level was decreased following autophagy-related 7 (Atg7) knockdown or increased using rapamycin. The data indicated that rapamycin had the ability to notably enhance the anticancer effect of DHA on MDA-MB-231 cells. Autophagy induction may be key in mediating the anticancer effects of DHA, and rapamycin may regulate the death-associated protein kinase via the alteration of Atg7 expression, which would influence cell apoptosis. The present study presented a novel insight into enhancing the effectiveness of future treatment regimens for breast cancer using DHA.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Rapamycin promotes the anticancer action of dihydroartemisinin in breast cancer MDA-MB-231 cells by regulating expression of Atg7 and DAPK

Liu

Zhou

et al. 2018

Oncol Lett

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“…Reports in the literature show that the anti-tumor effects of curcumin include not only the inhibition of tumor cell proliferation but also the induction of apoptosis [28]. Thus, we further tested whether LC could induce the apoptosis of EC cells.…”

Section: Discussionmentioning

confidence: 97%

Liposomal Curcumin Targeting Endometrial Cancer Through the NF-κB Pathway

Gong

Zhou

et al. 2018

Cell Physiol Biochem

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Background/Aims: Emerging evidence suggests that curcumin possesses chemopreventive properties against various cancers. However, its poor bioavailability limits its clinical application. In this study, we aimed to utilize encapsulation in liposomes (Lipo) as a strategy for the clinical administration of curcumin for endometrial carcinoma (EC). Methods: Curcumin was encapsulated in a liposomal delivery system to prepare a formulation of liposomal curcumin (LC). EC cell lines Ishikawa and HEC-1 were treated with the compound and cell proliferation was measured using MTT assay. Hoechst 33258 staining assay and flow cytometry were used to detect apoptosis of the cells. Wound healing and cell invasion assays were employed to monitor cell motility. Underlying target signaling, such as NF-κB, caspases, and MMPs, were further studied via qRT-PCR and western blot. Thereafter, a zebrafish model was used to assess the toxicity of LC. Finally, a zebrafish transplantation tumor model of EC was grown and treated with LC. Tumors were monitored and harvested to study the expression of NF-κB. Results: The formation of LC was successfully developed with excellent purity and physical properties. In vitro, LC resulted in dose-dependent inhibition of proliferation, induction of apoptosis, and suppression of Ishikawa and HEC-1 cell motility. LC treatment also suppressed the activation and/or expression of NF-κB, caspase-3, and MMP-9. No demonstrable toxicity was found in the zebrafish model and tumors were suppressed after treatment with LC. PCR analysis also showed down-regulated expression of NF-κB. Conclusions: LC was successfully prepared and played biological roles against EC probably through negative regulation of the NF-κB pathway in vitro and in vivo, which demonstrates its potential therapeutic effects in EC.

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“…The overexpression of miR-214 was reported in gastric [27] and cervical cancers [28, 29]. However, in most cases, miR-214 was downregulated and exerted an anti-oncogenic role [30-33]. For example, miR-214 suppressed the cell proliferation, migration, and invasion by targeting MEK3, JNK1, Plexin-B1, and GALNT7 [29, 34, 35] in cervical cancer cells.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-214 Suppresses Ovarian Cancer by Targeting β-Catenin

Liu

Jin

Zhai

et al. 2018

Cell Physiol Biochem

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Background/Aims: Ovarian cancer is one of the most common malignancies with a high rate of mortality in women. However, current therapies for ovarian cancer treatment are ineffective. Therefore, novel target identification is an urgent requisite. The present study aimed to investigate the role of microRNA-214 (miR-214) in ovarian cancer. Methods: The expression of miR-214, β-catenin, cyclin D1, c-myc, and TCF-1 at the transcriptional level was measured by real-time PCR, while that of β-catenin, Cyclin D1, and c-Myc at the protein level were detected by western blot. Colony formation assay and transwell assay were used to explore the invasion ability of the cancer cells. Cell cycle was measured by flow cytometry. Results: Real-time PCR showed that miR-214 expression in ovarian cancer cell lines was lower than that in the human normal ovarian epithelial cells, IOSE80. Furthermore, the low expression of miR-214 was correlated with high pathological grade. The rate of colony formation and invasion of miR-214 overexpression in SKOV-3 cells were weaker than that in control cells. Moreover, miR-214 overexpression led to the G0/G1 phase arrest. The expression of β-catenin, Cyclin D1, and c-Myc was suppressed by the overexpression of miR-214. Conclusion: These results suggested that miR-214 may serve as a tumor suppressor of ovarian cancer by targeting the β-catenin pathway.

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Dihydroartemisinin and Curcumin Synergistically Induce Apoptosis in SKOV3 Cells Via Upregulation of MiR-124 Targeting Midkine

Cited by 35 publications

References 35 publications

Rapamycin promotes the anticancer action of dihydroartemisinin in breast cancer MDA-MB-231 cells by regulating expression of Atg7 and DAPK

Rapamycin promotes the anticancer action of dihydroartemisinin in breast cancer MDA-MB-231 cells by regulating expression of Atg7 and DAPK

Liposomal Curcumin Targeting Endometrial Cancer Through the NF-κB Pathway

MicroRNA-214 Suppresses Ovarian Cancer by Targeting β-Catenin

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