Digestive proteases during development of larvae of red palm weevil, Rhynchophorus ferrugineus (Olivier, 1790) (Coleoptera: Curculionidae)

Alarcón, Francisco Javier; Martı́nez, T.F.; Barranco, P.; Cabello, Tomás; Díaz, Manuel; Moyano, Francisco Javier

doi:10.1016/s0965-1748(01)00087-x

Cited by 66 publications

(34 citation statements)

References 36 publications

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“…During larval development, maximum specific azocaseinase activity at pH 7.5-8.5 was detected in the 5th day of larval life, and zymogram showed the presence of two alkaline proteinases on the 3rd day, with accentuated activity on the 5th day, disappearing on the 6th day, coinciding with the pupae period, and appearing in the adult insect gut. Similar patterns of specific digestive proteinase activity were also reported throughout the larval development of Lepidoptera (Broadway and Duffey, 1985) and in Coleoptera (Michaud et al, 1995;Blanco-Labra et al, 1996;Novillo et al, 1997;Alarcon et al, 2002). In Diptera such as Lucilia cuprina (Casu et al, 1996) and Chrysomya bezziana (Muharsini et al, 2000), two serine proteinases in the larval lumen sensitive to specific inhibitors of trypsin-like enzymes were detected and isolated.…”

Section: Discussionsupporting

confidence: 70%

Digestive enzymes during development of Ceratitis capitata (Diptera:Tephritidae) and effects of SBTI on its digestive serine proteinase targets

Silva

Alcazar

Macedo

et al. 2006

Insect Biochemistry and Molecular Biology

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The digestive system of Ceratitis capitata was characterized during its larval development and in the insect stage. Disaccharidases against maltose and sucrose were more evident in the 2nd and 3rd day of larval development and in the adult stage, respectively. Glycosil-hydrolyses with higher specific a-galactosidasic and b-galactosidasic activities were detected in the 2nd and 3rd day of the larval stage, respectively. Specific proteolytic activities against azocasein showed an increase in the 4th and 5th day of the larval stage and in the adult stage. Specific hemoglobin activities were constant between 2nd and 6th day of the larval stage. The larvae used mainly serine proteinases, such as trypsin/chymotrypsin, and the adult insects only chymotrypsin-like enzymes in their digestive process. Two serine proteinases were separated from zymogram between the 4th and 5th day of larval development and in the adult stage. Effect of soybean trypsin inhibitor (SBTI, a serine proteinase inhibitor) on development of C. capitata was examined by bioassay. C. capitata was susceptible to SBTI which affected larval mass at ED 50 3.01%. r

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Section: Discussionsupporting

confidence: 70%

Digestive enzymes during development of Ceratitis capitata (Diptera:Tephritidae) and effects of SBTI on its digestive serine proteinase targets

Silva

Alcazar

Macedo

et al. 2006

Insect Biochemistry and Molecular Biology

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“…The total activity of alkaline proteases in the homogenate of the burbot intestinal mucosa and the activity of commercial trypsin sample (MP Biomedicals) were determined using 0.3% (w/v) azocasein in Tris buffer, pH 7.5 as substrate (Alarcón et al 2002). While preparing the control samples, the equivalent volume of buffer solution was added to each sample instead of enzymatically active components.…”

Section: Measuring the Proteolytic Activitymentioning

confidence: 99%

Inactivation of proteolytic enzymes by Eubothrium rugosum (Cestoda) from the gut of burbot Lota lota

2017

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Parasitic organisms inhabiting the alimentary canal should permanently resist the destructive action of host digestive enzymes. The intestinal parasites were shown to produce specific protease inhibitors protecting them from proteolysis. However, little is known about this adaptive mechanism in cestodes so far, especially for the tapeworms dwelling inside the fish intestines. Here, we explored the ability to inactivate proteolytic enzymes in the fish tapeworm Eubothrium rugosum (Batsch, 1786) (Bothriocephalidea) parasitising the intestine of wild burbot, Lota lota (Linnaeus). The assays were conducted with different concentrations of commercial trypsin and homogenate of intestinal mucosa both being the sources of proteinases. The incubation of live E. rugosum in trypsin solutions of two different concentrations caused a significant decrease in the enzyme activity. The extent of activity reduction was dependent on trypsin concentration. At the same time, the inhibitory effect of the worm incubation medium turned out to be statistically insignificant. These findings suggest partial adsorption of the enzyme to the tegument surface, with its further inactivation. In contrast to the incubation medium, the worm extract suppressed over 80% of trypsin activity and nearly half of the proteolytic activity in the mucosa homogenate. Notably, the inhibitory activity of the tapeworms hardly depended on their size characteristics. Finally, the research has demonstrated secretion of proteinase inhibitor in E. rugosum, which appears to be essential for its survival in enzymatically hostile environment.

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“…Adults were bred in pairs using sugarcane (Saccharum officinarum L.; Poales: Poaceae) as food and oviposition substrate. Eggs were collected from the sugarcane to sterile 9-cm diam plastic Petri dishes with an artificial food substrate (Alarcon et al 2002). Emerged larvae were individually transferred to 20 mL blood cell counter polystyrene vials with a 30 mL cap (VWR® International, Barcelona, Spain) containing 15 mL of artificial diet.…”

Section: Insectsmentioning

confidence: 99%

Effects of Combining Microbial and Chemical Insecticides on Mortality of the Pecan Weevil (Coleoptera: Curculionidae)

2011

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Digestive proteases during development of larvae of red palm weevil, Rhynchophorus ferrugineus (Olivier, 1790) (Coleoptera: Curculionidae)

Cited by 66 publications

References 36 publications

Digestive enzymes during development of Ceratitis capitata (Diptera:Tephritidae) and effects of SBTI on its digestive serine proteinase targets

Digestive enzymes during development of Ceratitis capitata (Diptera:Tephritidae) and effects of SBTI on its digestive serine proteinase targets

Inactivation of proteolytic enzymes by Eubothrium rugosum (Cestoda) from the gut of burbot Lota lota

Effects of Combining Microbial and Chemical Insecticides on Mortality of the Pecan Weevil (Coleoptera: Curculionidae)

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