2012
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Abstract: The major peanut allergen Ara h 1 is an easily digestible protein under physiological conditions. The present study revealed that pepsin digestion products of Ara h 1 retained the sensitizing potential in a Brown Norway rat model, while this sensitizing capacity was lost by separating the digest into fractions by gel permeation chromatography. Protein chemical analysis showed that the peptide composition as well as the aggregation profiles of the fractions of Ara h 1 digest differed from that of the whole pool… Show more

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Cited by 28 publications
(33 citation statements)
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References 53 publications
(134 reference statements)
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“…With fractionation of the peptide fragments by means of GPC it was demonstrated that especially the peptides composed of charged amino acid residues tended to form aggregates. This is in accordance with another study showing likewise that peptide fragments resulting from the digestion process of the peanut allergen Ara h 1 formed the largest complexes when composed mainly of charged and least of hydrophobic amino acid residues [16], indicating that non-covalent interactions other than hydrophobic are the main players in the formation of aggregates.…”
Section: Discussionsupporting
confidence: 92%
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“…With fractionation of the peptide fragments by means of GPC it was demonstrated that especially the peptides composed of charged amino acid residues tended to form aggregates. This is in accordance with another study showing likewise that peptide fragments resulting from the digestion process of the peanut allergen Ara h 1 formed the largest complexes when composed mainly of charged and least of hydrophobic amino acid residues [16], indicating that non-covalent interactions other than hydrophobic are the main players in the formation of aggregates.…”
Section: Discussionsupporting
confidence: 92%
“…Fractionation by preparative gel permeation chromatography (GPC) of gastro-duodenal digests of BLG was performed essentially as described in Bøgh et al [16]. Separated fractions were collected and pooled according to the preparative GPC profile shown in figure 1, resulting in four different pools of BLG digests in the following designation: partially digested BLG, digested BLG, large complexes and small complexes, where partially digested BLG corresponds to the whole pool of BLG digests, containing intact BLG that resisted the digestion process, and where digested BLG corresponds to the combined large and small complexes and thereby the whole pool of digested BLG without any intact BLG.…”
Section: Methodsmentioning
confidence: 99%
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