Differentiation of Mesenchymal Stem Cells Transplanted to a Rabbit Degenerative Disc Model

Sakai, Daisuke; Mochida, Joji; Iwashina, Toru; Watanabe, Takuya; Nakai, Takaaki; Ando, Kiyoshi; Hotta, Tomomitsu

doi:10.1097/01.brs.0000184365.28481.e3

Cited by 320 publications

(251 citation statements)

References 32 publications

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“…An attractive alternative for AF tissue engineering would be the use of hMSCs. However, while hMSCs can differentiate in NP cells [8,10,26], differentiation into AFCs has not been demonstrated yet. Sobajima et al [27] showed that, 24 weeks after MSCs transplantation in normal rabbit disc, MSCs were localized in the inner AF, where they exhibited an apparent change in morphology to a spindle shape more similar to native AFCs.…”

Section: Discussionmentioning

confidence: 99%

Bioactive electrospun scaffold for annulus fibrosus repair and regeneration

et al. 2012

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Purpose Annulus fibrosus (AF) tissue engineering is gathering increasing interest for the development of strategies to reduce recurrent disc herniation (DH) rate and to increase the effectiveness of intervertebral disc regeneration strategies. This study evaluates the use of a bioactive microfibrous poly(L-lactide) scaffold releasing Transforming Growth Factor (TGF)-b1 (PLLA/TGF) for the repair and regeneration of damaged AF. Methods The scaffold was synthesized by electrospinning, with a direct incorporation of TGF-b1 into the polymeric solution, and characterized in terms of morphology and drug release profile. Biological evaluation was performed with bovine AF cells (AFCs) that were cultured on the scaffold up to 3 weeks to quantitatively assess glycosaminoglycans and total collagen production, using bare electrospun PLLA as a control. Histological evaluation was performed to determine the thickness of the deposited neo-ECM. Results Results demonstrated that AFCs cultured on PLLA/TGF deposited a significantly greater amount of glycosaminoglycans and total collagen than the control, with higher neo-ECM thickness. Conclusions PLLA/TGF scaffold induced an anabolic stimulus on AFCs, mimicking the ECM three-dimensional environment of AF tissue. This bioactive scaffold showed encouraging results that allow envisaging an application for AF tissue engineering strategies and AF repair after discectomy for the prevention of recurrent DH.

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Section: Discussionmentioning

confidence: 99%

Bioactive electrospun scaffold for annulus fibrosus repair and regeneration

et al. 2012

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“…After differentiation, these cells can be placed on a gel scaffold or directly injected into the disc [73,88]. Use of bone marrowderived mesenchymal stem cells has demonstrated the ability to slow degeneration by preserving disc health as evaluated by MRI, histology, immunohistochemistry, and gene expression [44,[78][79][80]. These cells also demonstrated similar phenotypic activity to NP cells, including upregulation of aggrecan, versican, and type II collagen with suppression of the proinflammatory cytokines TNF-a and IL-1b [44].…”

Section: Stem Cell Therapiesmentioning

confidence: 99%

Molecular Basis of Intervertebral Disc Degeneration and Herniations: What Are the Important Translational Questions?

Kadow

Sowa

et al. 2015

Clinical Orthopaedics &Amp; Related Research

217

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Background Intervertebral disc degeneration is a common condition with few inexpensive and effective modes of treatment, but current investigations seek to clarify the underlying process and offer new treatment options. It will be important for physicians to understand the molecular basis for the pathology and how it translates to developing clinical treatments for disc degeneration. In this review, we sought to summarize for clinicians what is known about the molecular processes that causes disc degeneration.

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“…10 blade along the tail to expose the lateral portions of tail discs. Human MSCs (three passages, 1 9 10 6 cells/15 lL/ segment) (Sakai et al 2006(Sakai et al , 2005(Sakai et al , 2003 were then placed into TS using a stereotactic microinjector (Harvard Apparatus; Holliston, MA, USA) and a 26 G needle at 2 weeks post injury. The same amount of saline was injected into IS.…”

Section: Ivd Degeneration and The Transplantation Of Human Mesenchymamentioning

confidence: 99%

“…However, these approaches cannot be viewed as fundamental treatments for IVD degeneration because they do not address its cause. Recently, the molecular biological characteristics of IVD degeneration were identified, and treatment strategies are being sought that regenerate IVDs by restoring extracellular matrix or cellular components in animal models (Crevensten et al 2004;Le Visage et al 2006;Sakai et al 2006Sakai et al , 2005Sakai et al , 2003.…”

Section: Introductionmentioning

confidence: 99%

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Human mesenchymal stem cells implantation into the degenerated coccygeal disc of the rat

et al. 2009

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In this study, the authors explored the effect of human mesenchymal stem cell (MSC) implantation on the restoration of degenerative intervertebral discs (IVDs) in the rat. A unique rat coccygeal model was used to investigate the effects of transplanting human MSCs and to examine MSC survival in degenerative discs. MSC implantations into rat coccygeal IVDs were performed at 2 weeks postinjury. Radiologic and histologic evaluations were performed at 2, 4, 6, and 8 weeks post-injury. MSCinjected segments (TS) retained disc height and signal intensity, but injured non-injected segment (IS) progressively lost disc height. Pathological results revealed that the TS group showed relative restoration of the inner annulus structure; however, the IS group showed destruction of the inner annulus structure. Immunohistochemical staining using Anti-Human Nucleic Antibody (#MAB1281 Chemicon) revealed positive staining in the TS group at 2 weeks posttransplantation (4 weeks post-injury). This study shows that human MSCs survive for 2 weeks after transplantation into the IVDs of rats, and that MSCs increased the heights and signal intensities of intervertebral disc.

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Differentiation of Mesenchymal Stem Cells Transplanted to a Rabbit Degenerative Disc Model

Cited by 320 publications

References 32 publications

Bioactive electrospun scaffold for annulus fibrosus repair and regeneration

Bioactive electrospun scaffold for annulus fibrosus repair and regeneration

Molecular Basis of Intervertebral Disc Degeneration and Herniations: What Are the Important Translational Questions?

Human mesenchymal stem cells implantation into the degenerated coccygeal disc of the rat

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