Differentiation of grapevine (Vitis viniferaL.)concultamembers based on molecular tools

Abstract

Twenty-seven grapevine (Vitis vinifera L.) varieties within 12 putative berry colour variation groups (conculta) were genotyped with 14 highly polymorphic microsatellite (simple sequence repeats (SSR)) markers. Three additional oligonucleotide primers were applied for the detection of the Gret1 retroelement insertion in the promoter region of VvMybA1 transcription factor gene regulating the UFGT (UDP-glucose: flavonoid 3-O-glucosyltransferase) activity. UFGT is the key enzyme of the anthocyanin biosynthetic pathway. SSR results proved that the analysed cultivars can be grouped only into nine concultas, the other three putative berry colour variant groups consist of homonyms as a consequence of misnaming. In the case of Sárfehér-Sárpiros, Delaware red-Delaware white and Járdovány fekete-Járdovány fehér, it was attested that they are not bud sports, but homonyms. Some conculta members could be differentiated according to the presence or the absence of the Gret1 retroelement (Chasselas, Furmint and Lisztes), while others, Bajor, Bakator, Gohér and Traminer conculta members, remained indistinguishable either by the microsatellites or the Gret1-based method.

Genetic Diversity, Population Structure, and Parentage Analysis of Croatian Grapevine Germplasm

“…This resulted in the creation of a database of 2152 non-redundant genotypes, including 100 Croatian accessions previously genotyped through the GrapeGen06 project [ 7 ]. In addition, 1906 genetic profiles from publications containing shared loci [ 11 , 14 , 26 , 29 , 30 , 31 , 32 , 33 , 36 , 37 , 60 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 ] have been manually harmonized using common genotypes and added to the database for further analysis. Comparing all alleles across loci using the Excel-Microsatellite Toolkit [ 95 ], two conditions were identified: genotypes’ uniqueness and/or synonymy/duplication.…”

Compounds with Antiviral, Anti-Inflammatory and Anticancer Activity Identified in Wine from Hungary’s Tokaj Region via High Resolution Mass Spectrometry and Bioinformatics Analyses

“…In Hungary, wines produced in the Tokaj Hegyalja region, a declared UNESCO World Heritage site, are classified as Hungaricum [ 8 ]. “Furmint” is a traditional Hungarian grapevine variety, and one of the components of the world famous “aszú” wine [ 9 ]. “Tokaji aszú” is produced by a special process called noble rotting of the grapes.…”

Design and validation of an STR hexaplex assay for DNA profiling of grapevine cultivars

“…DNA profiling based on microsatellites (STR or simple sequence repeats, SSR) is the preferred way of identifying the cultivar of Vitis vinifera L. samples because the data generated are objective, more precise than those obtained using traditional ampelographic techniques , and less expensive than analysis using SNP arrays or genotyping by next‐generation sequencing . It also has advantages over profiling of grapevine must proteins , anthocyanines , amino acids , aromatic compounds , and chemical elements because the microsatellite profile is not affected by environmental factors such as a soil composition, weather conditions, or vinification .…”