Differentiation of Bartonella-like isolates at the species level by PCR-restriction fragment length polymorphism in the citrate synthase gene

Norman, Anthonyw .; Regnery, Russell L.; Jameson, Perry H.; Greene, Craig E.; Krause, Duncan C.

doi:10.1128/jcm.33.7.1797-1803.1995

Cited by 476 publications

(239 citation statements)

References 26 publications

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“…The DNA of each isolate was extracted using Viogene DNA/RNA Extraction Kit (Viogene Biotek Corp., Taipei, Taiwan) following the manufacturer's instructions. The primers BhCS.781p and BhCS.1137n were used for amplifying the partial fragment (approximately 390 bp) of the gltA gene of Bartonella species (Norman et al, 1995). The PCR-positive samples for the gltA gene were further analyzed by PCR of the 16S/23S rRNA intergenic spacer region (ITS) (Jensen et al, 2000).…”

Section: Dna Extraction and Polymerase Chain Reaction (Pcr) For Confimentioning

confidence: 99%

Identification of novel Bartonella spp. in bats and evidence of Asian gray shrew as a new potential reservoir of Bartonella

Lin

Hsu

Chomel

et al. 2012

Veterinary Microbiology

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Many studies indicated that small mammals are important reservoirs for Bartonella species. Using molecular methods, several studies have documented that bats could harbor Bartonella. This study was conducted to investigate the relationship of Bartonella spp. identified in bats and small mammals living in the same ecological environment. During May 2009 and March 2010, a total of 102 blood specimens were collected. By whole blood culture and molecular identification, a total of 6 bats, 1 rodent and 9 shrews were shown to be infected by Bartonella species. After sequencing and phylogenetic analyses of the sequences of gltA, ftsZ, rpoB and ribC genes, these specific isolates from bats were not similar to the known Bartonella species (the similarity values were less than 91.2%, 90.5%, 88.8%, and 82.2%, respectively); these isolates formed an independent clade away from other known Bartonella type strains. The Bartonella spp. isolated from small mammals, which were closely related to Bartonella tribocorum, Bartonella elizabethae, Bartonella grahamii, Bartonella rattimassiliensis and Bartonella queenslandensis, were similar to the findings in previous studies worldwide. Therefore, the results implied that the species of Bartonella strains isolated from small mammals were different from those identified in bats. Our results strongly suggested that the bat isolate could be a new Bartonella species. This study is also the first one to isolate Bartonella organisms from Asian gray shrews, Crocidura attenuata tanakae.

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Section: Dna Extraction and Polymerase Chain Reaction (Pcr) For Confimentioning

confidence: 99%

Identification of novel Bartonella spp. in bats and evidence of Asian gray shrew as a new potential reservoir of Bartonella

Lin

Hsu

Chomel

et al. 2012

Veterinary Microbiology

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“…Molecular detection and identification of Bartonella spp. DNA was based on citrate synthase gene (gltA) fragment amplification, which enables identification of a few Bartonella species, as previously published [1]. PCR products were purified with the QIAquick PCR Purification Kit (Qiagen).…”

Section: A T E R I a L S A N D M E T H O D Smentioning

confidence: 99%

Presence of Bartonella spp. in Ixodidae ticks

Podsiadły

Karbowiak

Tylewska-Wierzbanowska

2009

Clinical Microbiology and Infection

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Ixodes ricinus and Dermacentor reticulatus are common European ticks and vectors of various infections. Bartonella spp. is an aetiologic agent of vector-borne infections. Bartonella henselae transmission by cat fleas is now well established, and new potential vectors have recently been identified, including ticks and biting flies.The presence of Bartonella spp. was searched for in adult I. ricinus and D. reticulatus ticks collected from infested animals and vegetation. M A T E R I A L S A N D M E T H O D SThe studies were performed on 447 ticks. Two hundred and forty-two adult I. ricinus ticks, including 165 removed from animals and 77 collected from vegetation, were tested. All but one of the studied 205 adult D. reticulatus ticks came from vegetation; one was removed from a cow. The ticks were collected in various parts of Poland: central (Warsaw and suburbs), eastern (Bialowieza forest, Biala Podlaska) and southern (Radomsko).All ticks collected from vegetation were unfed. Among 165 Ixodes ricinus ticks collected from animals, 60 were engorged, 48 partly engorged and 55 unfed.Tick samples were stored at )70°C until DNA isolation by Qiagen columns (QIAamp DNA Mini Kit; Qiagen, Hilden, Germany). DNA was extracted from each tick in accordance with the manufacturer's protocol for tissue samples after homogenisation of the arthropod. All D. reticulatus ticks were crushed in Eppendorf tubes first and then DNA extraction was performed by boiling in 200 lL of 0.7 M NH 4 OH for 30 min.Molecular detection and identification of Bartonella spp. DNA was based on citrate synthase gene (gltA) fragment amplification, which enables identification of a few Bartonella species, as previously published [1]. PCR products were purified with the QIAquick PCR Purification Kit (Qiagen). The amplicons were sequenced with the ABI 377 DNA Analyzer (Applied Biosystem, Forester City, CA, USA). Database searches and sequence comparisons were performed with the BLAST search engines provided by the National Center for Biotechnology Information. R E S U L T SBartonella spp. DNA was detected in nine of 242 I. ricinus ticks, and in one of 205 D. reticulatus specimens. The frequency of Bartonella spp. in two studied species of ticks differed significantly. The majority of studied D. reticulates ticks were sampled from vegetation, whereas I. ricinus ticks were removed from both vegetation and animals.All of the nine I. ricinus ticks, in which Bartonella spp. DNA was detected, were removed from animals: eight from dogs and one from a cow. Four were engorged, four partly engorged and one unfed. The nucleotide sequence of the amplified fragments of the gltA gene from eight I. ricinus ticks were 99-100% homologous to sequences reported as B. henselae. In case of amplicon from one tick we got over 95% of homology with uncultured Bartonella sp. reported from I. tasmani and I. scapularis ticks.A D. reticulatus tick that was positive for Bartonella spp. DNA was collected from vegetation in Czerniakowski Park in Warsaw. A sequence of PCR-derived DNA fragmen...

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“…The fluid was collected and stored at −80°C until used as a DNA template. A PCR was performed using the genus-specific primer pair of RpCS877, 5′-GGGGGCCTGCTCACGGCGG and RpCS1258, 5′-ATT-GCAAAAAGTACAGTGAACA for the rickettsial citrate synthase (gltA) gene [25]. The reaction was carried out using TaKaRa Ex Taq (TAKARA BIO, Otsu, Japan) with a 20 µl reaction mixture containing 2 µl of template DNA, 200 µM each of dNTP and 1 µM each of primer.…”

Section: Methodsmentioning

confidence: 99%

Detection of rickettsial DNA in ticks and wild boars in Kyoto City, Japan

Someya

Ito

Maeda

et al. 2015

The Journal of Veterinary Medical Science

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The tick is a well-known vector for arthropod-borne pathogens, such as tick-borne encephalitis, Lyme disease, Japanese spotted fever and severe fever with thrombocytopenia syndrome. It is therefore important to know the tick population and distribution in our environment and wild animals in order to prevent tick-borne diseases. Here, we report the results of tick surveillance from May to September 2011 at 14 geographical points and in 5 wild boars in Kyoto City, Kyoto prefecture, Japan. We collected 3,198 ticks comprising 5 tick species, Haemaphysalis (H.) longicornis, H. flava, H. kitaokai, Amblyomma testudinarium and Dermacentor taiwanensis. Interestingly, the proportion of tick species varied according to geographical region within the city. The ticks collected in the city were reported as potential vectors of pathogens, such as rickettsiosis. We detected rickettsial DNA by PCR in 71.1% of 201 ticks investigated. The ticks that carried rickettsiae were distributed across the whole the city. The sequences of PCR-amplified DNA fragments were determined and showed similarities to spotted fever group rickettsiae. Although their pathogenicity for animals including humans is still unclear, it is important to stay alert and pay attention to tick-borne diseases in order to ensure the safety of the citizens of the city as well as that of visitors.

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Differentiation of Bartonella-like isolates at the species level by PCR-restriction fragment length polymorphism in the citrate synthase gene

Cited by 476 publications

References 26 publications

Identification of novel Bartonella spp. in bats and evidence of Asian gray shrew as a new potential reservoir of Bartonella

Identification of novel Bartonella spp. in bats and evidence of Asian gray shrew as a new potential reservoir of Bartonella

Presence of Bartonella spp. in Ixodidae ticks

Detection of rickettsial DNA in ticks and wild boars in Kyoto City, Japan

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