Differentiation of Avian Pathogenic <i>Escherichia coli</i> Strains from Broiler Chickens by Multiplex Polymerase Chain Reaction (PCR) and Random Amplified Polymorphic (RAPD) DNA

Abstract

We examined 50 Escherichia coli (E. coli) strains isolated from broiler chickens between January 2013 to March 2014 in order to evaluate the epidemiological prevalence of avian pathogenic E. coli (APEC) in Jordan by multiplex PCR and random amplification of polymorphic DNA (RAPD) tests. The multiplex polymerase chain reaction (PCR) which was used as tentative criteria of APEC targets 8 virulence associated genes; enteroaggregative toxin (astA), Type 1 fimbria adhesion (fimH), iron-repressible protein (irp2), P fimbriae (papC), aerobactin (iucD), temperature-sensitive hemagglutinin (tsh), vacuolating autotransporter toxin (vat), and colicin V plasmid operon (cva/cvi) genes. The number of detected genes could be used as a reliable index of their virulence. E. coli strains already typed as an APEC always harbor 5 to 8 genes, but non-APEC strains harbor less than 4 genes. Assuming the criteria of an APEC is possession of 5 or more virulence associated genes; we found that all 50 E. coli strains were classified as APEC strains. The RAPD analysis showed that the E. coli strains could be grouped into 35 of RAPD types by using these two different RAPD primer sets, RAPD analysis primer 4 5'AAGAGCCCGT5', and RAPD analysis primer 6 5'CCCGTCAGCA3'. The current study confirmed the endemic nature of APEC in broiler flocks in Jordan. It is essential that the biosecurity on poultry farms should be improved to prevent the introduction and dissemination of APEC and other agents. Furthermore, farmers need to be educated about the signs, lesions, and the importance of this agent.

Molecular Characterization of Some Bacteria Isolated from Broiler Chickens Showing Respiratory Manifestations

“…The recovered results showed that presence of tsh in E. coli isolated from broiler chickens with respiratory manifestations at a percentage of 30% that agree with the result reported by Oh et al, (2011) who found that PCR for amplification of tsh gene of E. coli at a percentage of34.48% and lower than the result obtained by (Roussan et al, 2014) who identified tsh gene with a percentage (66%) by using PCR.…”

“…This result is higher than the result that said by (Mbanga and Nyararai, 2015) who said that percent of PCR for amplification of fimH gene of E. coli was (33.3%) of APEC isolates and lower than the result obtained by (Roussan et al, 2014) who identified fimH gene with a percentage (94%) by using PCR.…”

Molecular characterization and antibiotic resistance patterns of avian fecal Escherichia coli from turkeys, geese, and ducks

“…On the other hand, APEC isolates can bind to avian tracheal cells and Type I fimbriae confer this ability. A study on APEC showed a 94% prevalence of fimH gene [ 33 ]. The fimH is claimed to be responsible for the first step in the colonization of the lungs and air sacs of birds.…”

Bacteriophage activity against and characterisation of avian pathogenicEscherichia coliisolated from colibacillosis cases in Uganda

“…Escherichia coli strains possess various virulence factors for extra-intestinal survival [4][5][6][7][8]. However, capacity to cause disease in a specific host species, depends on acquisition of appropriate virulence gene combination by a given E. coli strain [5].…”

“…However, capacity to cause disease in a specific host species, depends on acquisition of appropriate virulence gene combination by a given E. coli strain [5]. The number of detected genes can be used as a reliable index of their virulence; and strains typed as APEC possess five to eight genes, while the non-APEC ones harbor less than four genes [5,8]. Virulence-associated genes, namely, iutA, hlyF, iss, iroN, and ompT were suggested as the minimum that can be used to identify an APEC strain with the highest pathogenicity [9].…”