Differentiation capacity of dental pulp stem cell into inner ear hair cell using an in vitro assay: a preliminary step toward treating sensorineural hearing loss

Adriztina, Indri; Munir, Delfitri; Sandra, Ferry; Ichwan, Muhamad; Bashiruddin, Jenny; Putra, Imam Budi; Farhat, Farhat; Sembiring, Rosita Juwita; Sartika, C.R.; Chouw, Angliana; Pratiwi, Endah Dianty

doi:10.1007/s00405-021-06864-9

Cited by 4 publications

(4 citation statements)

References 25 publications

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“…This result demonstrates that auditory brainstem slices offer a microenvironment that promotes survival, expansion and neurogenesis of transplanted stem cells. A similar differentiation approach was adopted in another study, where DPSCs were induced to differentiate into cochlear hair cells [ 64 ]. Researchers first induced the neuronal lineage by the addition of b-FGF and EGF for a week.…”

Section: Relevance Of Dpscs and Sheds In Neurodegenerative Diseasesmentioning

confidence: 99%

The Importance of Stem Cells Isolated from Human Dental Pulp and Exfoliated Deciduous Teeth as Therapeutic Approach in Nervous System Pathologies

Candelise,

Santilli,

Fabrizi

et al. 2023

Cells

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Despite decades of research, no therapies are available to halt or slow down the course of neuro-degenerative disorders. Most of the drugs developed to fight neurodegeneration are aimed to alleviate symptoms, but none has proven adequate in altering the course of the pathologies. Cell therapy has emerged as an intriguing alternative to the classical pharmacological approach. Cell therapy consists of the transplantation of stem cells that can be obtained from various embryonal and adult tissues. Whereas the former holds notable ethical issue, adult somatic stem cells can be obtained without major concerns. However, most adult stem cells, such as those derived from the bone marrow, are committed toward the mesodermal lineage, and hence need to be reprogrammed to induce the differentiation into the neurons. The discovery of neural crest stem cells in the dental pulp, both in adults’ molar and in baby teeth (dental pulp stem cells and stem cells from human exfoliated deciduous teeth, respectively) prompted researchers to investigate their utility as therapy in nervous system disorders. In this review, we recapitulate the advancements on the application of these stem cells in preclinical models of neurodegenerative diseases, highlighting differences and analogies in their maintenance, differentiation, and potential clinical application.

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Section: Relevance Of Dpscs and Sheds In Neurodegenerative Diseasesmentioning

confidence: 99%

The Importance of Stem Cells Isolated from Human Dental Pulp and Exfoliated Deciduous Teeth as Therapeutic Approach in Nervous System Pathologies

Candelise,

Santilli,

Fabrizi

et al. 2023

Cells

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“…In a recent study, differentiation of DPSCs into cochlear hair cells was demonstrated, suggesting their potential as a regenerative therapy candidate for neural diseases such as sensorineural hearing loss (SNHL), which is primarily caused by dysfunction or death of cochlear cell types, due to their lack of regenerative capacity [ 165 ]. In another study, a rat model with stress urinary incontinence had human dental pulp stem cells (pre-differentiated) engrafted in the external urethral sphincter, which recovered its thickness, promoted vascularization and, exhibited significant recovery of urinary incontinence [ 93 ].…”

Section: Oral Stem Cell-mediated Regeneration Of Other Tissuesmentioning

confidence: 99%

Botanicals and Oral Stem Cell Mediated Regeneration: A Paradigm Shift from Artificial to Biological Replacement

Ahuja

Tyagi

Kumar

et al. 2022

Cells

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Stem cells are a well-known autologous pluripotent cell source, having excellent potential to develop into specialized cells, such as brain, skin, and bone marrow cells. The oral cavity is reported to be a rich source of multiple types of oral stem cells, including the dental pulp, mucosal soft tissues, periodontal ligament, and apical papilla. Oral stem cells were useful for both the regeneration of soft tissue components in the dental pulp and mineralized structure regeneration, such as bone or dentin, and can be a viable substitute for traditionally used bone marrow stem cells. In recent years, several studies have reported that plant extracts or compounds promoted the proliferation, differentiation, and survival of different oral stem cells. This review is carried out by following the PRISMA guidelines and focusing mainly on the effects of bioactive compounds on oral stem cell-mediated dental, bone, and neural regeneration. It is observed that in recent years studies were mainly focused on the utilization of oral stem cell-mediated regeneration of bone or dental mesenchymal cells, however, the utility of bioactive compounds on oral stem cell-mediated regeneration requires additional assessment beyond in vitro and in vivo studies, and requires more randomized clinical trials and case studies.

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“…The high rate of hearing loss in Southeast Asia has affected people's quality of life ( 1 , 2 ). Sensorineural hearing loss is the most frequent type and can be caused by death or dysfunction of cochlear cell types, including hair cells ( 3 ), which is often attributed to oxidative stress ( 4 ). Previous research suggested that decreased vascularity and endothelial dysfunction are pathogenic mechanisms for many otologic disorders, including sensorineural hearing loss ( 5 ).…”

Section: Introductionmentioning

confidence: 99%

The Effect of the Intra-Arterial Heparin Flushing (IAHF) Procedure on Hearing Threshold in Sensorineural Hearing Loss Patients

Daulay,

Munir,

Lelo

et al. 2023

Med Arch

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Background: Sensorineural hearing loss (SNHL) is a frequent problem in Indonesia but its treatment is still limited. This type of hearing loss is related to oxidative stress and decreased vascularization, which can damage the hair cell. The intra-arterial heparin flushing (IAHF) is a procedure that can recover circulation and its agent, namely heparin, also has antioxidant activity. Therefore, the IAHF procedure has the potential to improve hearing function and can be considered an alternative therapy for SNHL.Objective: The study evaluates the effect of the IAHF on hearing improvement based on the difference in hearing threshold values before and after the procedure. Methods: This experimental study used a Pretest-Posttest One-Group Only design. A total of 17 patients with sensorineural hearing loss who met inclusion and exclusion criteria were subjected to pure tone audiometry tests before and 4 hours after the IAHF procedure. The mean difference in hearing threshold was analyzed using paired Students t-test for normally distributed data and Wilcoxon for non-normally distributed data. Results: There was a decrease in the means of hearing threshold in the right and left ear 4 hours after the IAHF procedure. However, based on the paired Students t-test, there was not a significant difference in hearing threshold before and after the procedure (p-value > 0.05). Conclusion: There was hearing threshold improvement 4 hours after the IAHF procedure. This study showed that the IAHF procedure can have a therapeutic effect on sensorineural hearing loss patients.

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Differentiation capacity of dental pulp stem cell into inner ear hair cell using an in vitro assay: a preliminary step toward treating sensorineural hearing loss

Cited by 4 publications

References 25 publications

The Importance of Stem Cells Isolated from Human Dental Pulp and Exfoliated Deciduous Teeth as Therapeutic Approach in Nervous System Pathologies

The Importance of Stem Cells Isolated from Human Dental Pulp and Exfoliated Deciduous Teeth as Therapeutic Approach in Nervous System Pathologies

Botanicals and Oral Stem Cell Mediated Regeneration: A Paradigm Shift from Artificial to Biological Replacement

The Effect of the Intra-Arterial Heparin Flushing (IAHF) Procedure on Hearing Threshold in Sensorineural Hearing Loss Patients

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