Differentiation between some Ulva spp. by morphological, genetic and biochemical analyses

Ismail, Mona M.; Mohamed, Ehab

doi:10.18699/vj17.253

Cited by 11 publications

(8 citation statements)

References 23 publications

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“…The present investigation paid great attention to the biochemical characterization of collecting seaweeds; a high diversity of the biochemical nature of seaweeds paves the path to explore a variety of compounds in their body composition. Generally, carbohydrates represent the major biochemical component in the three seaweeds, this may be due to higher pyrocollodion content in their cell walls (Ismail and Mohamed, 2017;Mendis and Kim, 2011). The changes in the biochemical content may be spatial or temporal but dominantly attributed to water quality (Dave and Parekh, 1975).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial activities of three seaweeds extract against some human viral and bacterial pathogens

Mofeed¹,

Deyab²,

Mohamed³

et al. 2022

Biocell

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Microbial infections cause complicated health influences along with bad economic impacts. In the present investigation, three dominant seaweeds namely, Amphiroa anceps, Corallina officinalis and Sargassum filipendula were collected from different Egyptian sites at the Red Sea and Mediterranean Sea during autumn 2019. Organic extracts of the three algae were screened for their antibacterial activity against three pathogenic bacteria Salmonella typhiimurium, Staphylococcus aureus and Escherichia coli, in addition to in vitro antiviral activity against Rotavirus (RV), and Coxsackie virus B3 (CVB3) that cause severe diseases in human. Organic extract of A. anceps, C. officinalis and S. filipendula inhibit E. coli cells by 57.1%, 85.7%, and 91.4%, respectively. The highest level of concentration of the C. officinalis extract (100 µg/mL) inhibits 100% of Staphylococcus aureus cells followed by S. filipendula and A. anceps extract which inhibit 82.5% and 75% of S. aureus. Similarly, the highest concentration of C. officinalis extract inhibits S. typhiimurium by 80%. The extract of A. anceps exhibited a high antiviral effect against RV infection with TI = 22 and virus titers lessened by 2.75 log TCID 50 followed by extractions of C. officinalis with TI = 18.3 and virus titers reduced by 2.5 log TCID 50 . Against CVB3 infection, the extract of A. anceps causes the highest antiviral activity with TI = 15 and reduce the viral titers by 2.5 log TCID 50 , followed by extractions of C. officinalis with TI = 8.8 and inhibition of virus titers by 1.75 log TCID 50 . Extract of S. filipendula displayed the lowest antiviral effects against RV and CVB3 infection with TI = 2.4 and 1.4, respectively. The obtained results clarified that the extract of three marine seaweeds maintains a potent antimicrobial activity, making them a future promising source of new antimicrobial drugs.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial activities of three seaweeds extract against some human viral and bacterial pathogens

Mofeed¹,

Deyab²,

Mohamed³

et al. 2022

Biocell

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“…The combination of molecular and morphological analyses provides a strong and more accurate way to differentiate between organisms. In addition, biochemical analyses have been proved to be effective in discrimination of different algal species (Ismail and Mohamed, 2017).…”

Section: Morphologicalmentioning

confidence: 99%

Impact of Different Geographical Locations on Genetic Variation and Phytochemical Constituents of Two Medicinal Marine Algae.

Saleh

El-Atroush

2020

Taeckholmia

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Genetic variations and phytochemical constituents of Dictyota dichotoma and Sargassum dentifolium, collected from Hurghada and Abu-Qir bay, were investigated. Results revealed an increase in the concentrations of carotenoids, sugars and DPPH% in the samples of both algal species collected from Hurghada as compared with those collected from Abu-Qir. Regarding the samples collected from Hurghada, an increase in the concentrations of chlorophyll a, b and phenols was recorded in S. dentifolium; while a decrease in chlorophyll a, b only was observed in D. dichotoma. No significant difference was observed in phenol concentrations between D. dichotoma samples collected from the two studied localities. DNA was isolated and thirteen SCoT molecular markers were used to detect the genetic variations between the samples of the two localities. In D. dichotoma, the total average of polymorphism is 56.68%. SCoT-1 exhibited the highest polymorphism (80%), while SCoT-28 recorded the lowest polymorphism (35.71%). In S. dentifolium, the total average of polymorphism is 61.63%. SCoT-3 showed the highest polymorphism (90%), while SCoT-13 exhibited the lowest polymorphism (0.09%). In addition, BSI factor showed a marked difference between the two studied algae that it was 0.87 % in D. dichotoma and 0.77 % in S. dentifolium. Variation in geographical location affects genetic variation and phytochemical composition of D. dichotoma and S. dentifolium. The difference in the responses of the two studied algae to the change of locality and environmental conditions may reflect differences in their gene expression. Moreover, the efficiency of SCoT markers to discriminate between algal species collected from different localities has been confirmed.

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“…U. rigida can be found in Eastern Atlantic, Caribbean, India and Pacific Oceans [3]. Melton and Lopez-Bautista [4], found that U. rigida was distributed in the Gulf of Mexico and Atlantic side of the U.S.A. Ismail and Mohamed [5], studied the cell surface of Ulva spp. under a light microscope and reported that the cells were varied in sizes and shapes.…”

Section: Introductionmentioning

confidence: 99%

“…The identification of alga species requires a combination of molecular markers and morphological structures [11]. In addition, the morphological characters of Ulva can change with impacting environmental factors and developmental stages of the alga [5]. Ulva can grow in freshwater, brackish and saline environments [12].…”

Section: Introductionmentioning

confidence: 99%

Structural Development of Marine Green Alga (Ulva rigida C. Agardh, 1823) during Cultivation

et al. 2023

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Ulva rigida C. Agardh (sea lettuce) is a marine green alga. It grows naturally near shorelines in areas with freshwater influence. It can also be cultivated easily on farms and can be developed into many healthy food products, which bring in revenue for Thailand. It is important to develop an effective way to cultivate the alga. In this research, the molecular biology of sea lettuce using the tufA gene for the identification of Ulva genus and morphological characters at different culturing periods of U. rigida thallus were investigated using a light microscope (LM), a scanning electron microscope (SEM) and a transmission electron microscope (TEM). The results revealed that the U. rigida alga consisted of various cell shapes including elliptic, irregular triangle, irregular quadrilateral, pentagonal and polygonal. The cell size increased with increasing periods of culture. However, when the alga was cultured for 28 days, the cell sizes were smaller than the original cells due to cell division during aging, resulting in an increase in cell numbers. U. rigida had grown rapidly in 14 days which was a relatively short period of cultivation. The results of this study indicate that U. rigida has a potential to be scaled up in pilot and commercial scales. HIGHLIGHTS The molecular analysis of sea lettuce from Phetchaburi Coastal Aquaculture Research and Development Center, Sub District: Bang Kaeo, District: Ban Laem at Phetchaburi Province, Thailand was found to be rigida The morphological and anatomical characters of rigida thalli were studied with light microscope, transmission electron microscope and scanning electron microscope techniques The cell sizes of rigida thalli changed with culturing days. They were the largest at 14 days in the surface view, and at 14 and 21 days in the transverse section GRAPHICAL ABSTRACT

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Differentiation between some Ulva spp. by morphological, genetic and biochemical analyses

Cited by 11 publications

References 23 publications

Antimicrobial activities of three seaweeds extract against some human viral and bacterial pathogens

Antimicrobial activities of three seaweeds extract against some human viral and bacterial pathogens

Impact of Different Geographical Locations on Genetic Variation and Phytochemical Constituents of Two Medicinal Marine Algae.

Structural Development of Marine Green Alga (Ulva rigida C. Agardh, 1823) during Cultivation

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