Differentiation and plasticity of human vascular wall mesenchymal stem cells, dermal fibroblasts and myofibroblasts: a critical comparison including ultrastructural evaluation of osteogenic potential

Pasanisi, Emanuela; Ciavarella, Carmen; Valente, Sabrina; Ricci, Francesca; Pasquinelli, Gianandrea

doi:10.1080/01913123.2019.1673863

Cited by 9 publications

(13 citation statements)

References 34 publications

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“…hRIFs are one of the main cells in kidney interstitial tissue. Studies have shown that the fibroblasts in multiorganization have osteogenic differentiation potential (34,35). However, there are no reports on the osteogeniclike differentiation of hRIFs.…”

Section: Discussionmentioning

confidence: 99%

Suppression of osteogenic-like differentiation in human renal interstitial fibroblasts by miRNA-410-3p through MSX2

Zhu¹,

Huang²,

Chen³

et al. 2020

Transl Androl Urol

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Background: The aim of this stay was to determine the effect of calcium ions in promoting osteogenic-like differentiation in human renal interstitial fibroblasts (hRIFs). The role of miRNA-410-3p in upregulating Msh homeobox 2 (MSX2) level in hRIFs was also investigated.Methods: Quantitative polymerase chain reaction (qPCR) analysis was used to assess the expression levels of miRNA-410-3p in Randall's plaque (RP) and normal renal papillary (nRP) tissues. Furthermore, the expression levels of osteogenesis-related protein in the RP and nRP tissues were assessed with qPCR and immunohistochemistry (IHC). hRIFs were cultured from isolated human kidney papilla before treatment with calcium chloride or osteogenic medium, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed at 1, 5, 9, and 14 days post-treatment. Alizarin red staining was used to estimate the deposits of calcium aggregates. After the overexpression or knockdown of miRNA-410-3p, we evaluated the changes in the osteogenic-like differentiation and osteogenesis-related protein by alizarin red staining and qPCR, respectively. A binding relationship between miRNA-410-3p and MSX2 was established through a dual-luciferase reporter gene assay. Rescue experiments demonstrated that miRNA-410-3p regulated the osteogenic-like differentiation by targeting MSX2.Results: miRNA-410-3p levels were lower in RP tissue than in control nRP tissues. qPCR and IHC showed that the level of runt-related transcription factor 2 (Runx2), osteocalcin (OCN), and osteopontin (OPN) were higher in RP tissues. The calcium deposition of hRIFs showed a time-dependent trend when treated with osteogenic medium or calcium chloride. The overexpression of miRNA-410-3p downregulated the levels of osteogenesis-related expression and attenuated mineralization. The knockdown of miRNA-410-3p yielded the opposite trend. Dual-luciferase reporter gene assay and rescue experiments indicated that miRNA-410-3p could target MSX2, while the overexpression of MSX2 reversed the effects of miRNA-410-3p on osteogenic-like differentiation. Conclusions:The current findings suggest that calcium ions could promote the osteogenic-like differentiation of hRIFs and miRNA-410-3p regulates hRIFs osteogenic-like differentiation by inhibiting MSX2.

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Section: Discussionmentioning

confidence: 99%

Suppression of osteogenic-like differentiation in human renal interstitial fibroblasts by miRNA-410-3p through MSX2

Zhu¹,

Huang²,

Chen³

et al. 2020

Transl Androl Urol

View full text Add to dashboard Cite

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Section: Common Properties: Tissue Remodeling and Immunomodulationmentioning

confidence: 99%

“…In contrast, there are also studies that have provided evidence of greater antiinflammatory and wound-healing features of MSCs in comparison to other stromal cells [25]. In an array of in-vitro tests to compare human artery-wall-derived MSCs with dermal fibroblasts and myofibroblasts, Pasanisi et al showed some profound differences in the immunomodulatory properties between these cell types [25]. Both the dermal fibroblasts and myofibroblasts expressed very low levels of immunomodulatory and inflammation-related genes, and had lower immunosuppressive potential for proliferation of peripheral blood mononuclear cells in comparison to the femoral artery MSCs.…”

Section: Common Properties: Tissue Remodeling and Immunomodulationmentioning

confidence: 99%

“…Both the dermal fibroblasts and myofibroblasts expressed very low levels of immunomodulatory and inflammation-related genes, and had lower immunosuppressive potential for proliferation of peripheral blood mononuclear cells in comparison to the femoral artery MSCs. They also suggested that the two highly sought after translational abilities, as anti-inflammatory and wound healing activities, are unique features of MSCs [25].…”

Section: Common Properties: Tissue Remodeling and Immunomodulationmentioning

confidence: 99%

“…Hence, the minimal criteria set by the International Society for Cellular Therapy to define MSCs [6] can also define fibroblasts [20]. Despite great effort, the lack of a specific marker to distinguish between MSCs and fibroblasts represents a major limit in the study of these cells [25].…”

Section: Common Properties: Tissue Remodeling and Immunomodulationmentioning

confidence: 99%

See 2 more Smart Citations

Mesenchymal Stem/Stromal Cells and Fibroblasts: Their Roles in Tissue Injury and Regeneration, and Age-Related Degeneration

Zupan¹

2021

Fibroblasts - Advances in Inflammation, Autoimmunity and Cancer

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Mesenchymal stem/stromal cells (MSCs) and fibroblasts are present in normal tissues to support tissue homeostasis. Both share common pathways and have a number of common features, such as a spindle-shaped morphology, connective tissue localization, and multipotency. In inflammation, a nonspecific response to injury, fibroblasts and MSC are the main players.Two mechanisms of their mode of action have been defined: immunomodulation and regeneration. Following tissue injury, MSCs are activated, and they multiply and differentiate, to mitigate the damage. With aging and, in particular, in degenerative disorders of the musculoskeletal system (i.e., joint and bone disorders), the regenerative capacity of MSCs appears to be lost or diverted into the production of other nonfunctional cell types, such as adipocytes and fibroblasts. Fibroblasts are stromal cells that provide the majority of the structural framework of almost all types of tissues; i.e., the stroma. As such, fibroblasts also have significant roles in tissue development, maintenance, and repair. In their immunosuppressive role, MSCs and fibroblasts contribute to the normal resolution of inflammation that is a prerequisite for successful tissue repair. In this chapter, we review the common and opposing properties of different tissue-derived MSCs and fibroblasts under physiological and pathophysiological conditions. We consider injury and age-related degeneration of various tissues, and also some immunological disorders. Specifically, we address the distinct and common features of both cell types in health and disease, with a focus on human synovial joints. Finally, we also discuss the possible approaches to boost the complementary roles of MSCs and fibroblasts, to promote successful tissue regeneration.

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Human Adipose-Derived Microvessel Fragments: A Natural Vascularization Units for Ischemic Diseases

You,

Chen,

et al. 2024

Aesth Plast Surg

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Differentiation and plasticity of human vascular wall mesenchymal stem cells, dermal fibroblasts and myofibroblasts: a critical comparison including ultrastructural evaluation of osteogenic potential

Cited by 9 publications

References 34 publications

Suppression of osteogenic-like differentiation in human renal interstitial fibroblasts by miRNA-410-3p through MSX2

Suppression of osteogenic-like differentiation in human renal interstitial fibroblasts by miRNA-410-3p through MSX2

Mesenchymal Stem/Stromal Cells and Fibroblasts: Their Roles in Tissue Injury and Regeneration, and Age-Related Degeneration

Human Adipose-Derived Microvessel Fragments: A Natural Vascularization Units for Ischemic Diseases

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