Differentiation and expansion of endothelial cells from human bone marrow CD133⁺ cells

Abstract

Summary. We report a method of purifying, characterizing and expanding endothelial cells (ECs) derived from CD133

Formação in vitro de túbulos capilares a partir de células de sangue de cordão umbilical humano com perspectivas para aplicação terapêutica

“…EPCs can be obtained and expanded from HUCBs, and also used for several functions, such as ex vivo expansion of cells similar to ECs for cellular and gene therapy, or, according to Quirici et al [26], in in vitro co-cultures that can provide new perspectives for the treatment of ischemic heart disease. The results obtained by Melero-Martin et al [27] reaffirm SENEGAGLIA, AC ET AL -In vitro formation of capillary tubules from human umbilical cord blood cells with perspectives for therapeutic application the in vivo therapeutic potential of EPCs to form vascular networks that allow for the vascularization of ischemic tissues and organs.…”

Rapid Anastomosis of Endothelial Progenitor Cell–Derived Vessels with Host Vasculature Is Promoted by a High Density of Cotransplanted Fibroblasts

“…31 The circulating EPCs migrate to the sites of hypoxia or injured vessels, and incorporate into the sprouting neovessels or participate in the re-endothelialization of damaged vessels. Since the initial reports of separating EPCs from peripheral blood, 32 circulating EPCs have been isolated with antibodies specific to a variety of cell surface markers, including the progenitor=stem cell marker CD133, 33 the endothelial marker CD34, 32,34,35 CD31, 18,24 or a combination of endothelial and progenitor cell markers. 36 In this study, we purified the endothelial outgrowth of CD31-positive cells from cord blood, but EPCs derived from adult blood have also been shown to have equivalent angiogenic potential when implanted with bone marrow mesenchymal cells.…”

Flow cytometric evaluation of circulating endothelial cells: A new protocol for identifying endothelial cells at several stages of differentiation

“…At present, there is no standardized method for determining the number of circulating ECs, and there are no data on their in vivo lifespan or maturation process [10][11][12][13]. To investigate ECs, some authors have used adherence cultures of total mononuclear cells or mononuclear cells preselected by antibodies (inserted in magnetic microbeads) that bind to surface markers such as CD133, CD34, or CD31 [1,3,11,[14][15][16][17][18]. Most authors have used the monoclonal antibodies, CD146 or CD144, for identification of ECs, but not their combination.…”