Differentially expressed lncRNAs and miRNAs with associated ceRNA networks in aged mice with postoperative cognitive dysfunction

Wei, Changwei; Luo, Ting; Zou, Sheng; Zhou, Xiujuan; Shen, Wenzhen; Ji, Xiaolin; Li, Qi; Wu, Anshi

doi:10.18632/oncotarget.18362

Cited by 51 publications

(53 citation statements)

References 42 publications

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“…Previous studies have reported that several miRNAs were significantly associated with POCD [6,[18][19][20], which provide supportive evidence of the important role of miRNAs in POCD. Consistently, some of those reported miRNAs were replicated in our current study.…”

Section: Discussionsupporting

confidence: 72%

“…The miRNA expression profile data of replication cohort were downloaded from the Gene Expression Omnibus (GEO) database (Accession number: GSE95070; https:// www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE95070), which were deposited by Wei et al [19] from the Chaoyang Hospital. With regard to this dataset, hippocampus tissues of 10 mice (5 per group) were dissected, and the different miRNA expression levels between two groups were detected by Affymetrix miRNA 4.0 as well.…”

Section: Microarray and Statistical Analysismentioning

confidence: 99%

“…Furthermore, multiple aberrantly expressed miR-NAs were implicated in the development of Huntington's disease [13], neural tube defects [16], and Alzheimer's disease [17]. As for POCD, several miRNAs have been reported to be associated with it [6,[18][19][20], such as, Yu et al [6] reported that miR-572 was implicated in the development and restoration of POCD and might serve as a biological marker in early diagnosis of POCD. However, the specific roles and underlying mechanism of miRNAs in POCD are still not yet clear.…”

Section: Introductionmentioning

confidence: 99%

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MiR-190a potentially ameliorates postoperative cognitive dysfunction by regulating Tiam1

et al. 2019

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Background: Postoperative cognitive dysfunction (POCD) affects a large number of post-surgery patients, especially for the elderly. However, the etiology of this neurocognitive disorder is largely unknown. Even if several studies have reported a small number of miRNAs as the essential modulatory factors in POCD, these findings are still rather limited. The aim of current study was to screen the POCD-related miRNAs in the hippocampus tissues and investigate the target genes of differentially expressed miRNAs and their biological functions underlying POCD pathophysiology. Methods: The miRNA microarray was used to find the abnormal expression of miRNAs in the hippocampus tissues from the POCD model mice to normal mice (Discovery cohort, 3 vs 3). The nominal significant results were validated in an independent sample of hippocampus tissues of 10 mice based on same miRNA microarray (Replication cohort, 5 vs 5). Expression level of the most significantly abnormal miRNA was further validated by real-time quantitative polymerase chain reaction (PCR). To determine the expression pattern among miRNAs and genes and detect the interactions, we conducted a weighted gene co-expression network analysis (WGCNA) in the miRNAs and genes expression data from hippocampus tissue of wild type mice (n = 24). The target genes of miRNAs were predicted using the miRWalk3.0 software. Furthermore, we used the ClueGO software to decipher the pathways network and reveal the biological functions of target genes of miRNAs. Results: We found that nine miRNAs showed significant associations with POCD in both datasets. Among these miRNAs, mmu-miR-190a-3p was the most significant one. By performing WGCNA analysis, we found 25 coexpression modules, of which mmu-miR-190a-3p was significantly anti-correlated with red module. Moreover, in the red module, 314 genes were significantly enriched in four pathways such as axon guidance and calcium signaling pathway, which are well-documented to be associated with psychiatric disorders and brain development. Also, 169 of the 314 genes were highly correlated with mmu-miR-190a-3p, and four genes (Sphkap, Arhgef25, Tiam1, and Ntrk3) had putative binding sites at 3′-UTR of mmu-miR-190a-3p. Based on protein-protein network analysis, we detected that Tiam1 was a central gene regulated by the mmu-miR-190a-3p.

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Section: Discussionsupporting

confidence: 72%

Section: Microarray and Statistical Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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MiR-190a potentially ameliorates postoperative cognitive dysfunction by regulating Tiam1

et al. 2019

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“…Competing endogenous RNAs (ceRNA) network attracted more and more attention due to its significant effects on regulating the progression of cancers and nontumor diseases [10,11]. For example, HOTAIR served as a ceRNA to modulate HER2 expression via sponging miR-331-3p in GC [12].…”

Section: Introductionmentioning

confidence: 99%

LBX2-AS1 up-regulated by NFIC boosts cell proliferation, migration and invasion in gastric cancer through targeting miR-491-5p/ZNF703

Zhang

et al. 2020

Cancer Cell Int

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Background: The crucial role of long non-coding RNAs (lncRNAs) has been certified in human cancers. The lncRNAs with abnormal expressions could act as tumor inhibitors or oncogenes in the advancement of tumors. LBX2-AS1 was once reported to accelerate esophageal squamous cell carcinoma. Nonetheless, its function in gastric cancer (GC) remained a riddle. Methods: RT-qPCR was used to examine the expression of NFIC/LBX2-AS1/miR-491-5p/ZNF703 in GC cell lines. The functions of LBX2-AS1 in GC were appraised by colony formation, EdU, flow cytometry analysis, transwell and wound healing assays. Luciferase reporter, ChIP and RNA pull down assays were utilized to evaluate the interactions among genes. Results: LBX2-AS1 was up-regulated in GC cell lines. Knockdown of LBX2-AS1 repressed the proliferative, migratory, and invasive abilities of GC cells. Moreover, LBX2-AS1 was transcriptionally activated by NFIC. And LBX2-AS1 could bind with miR-491-5p. Besides, miR-491-5p depletion or ZNF703 upregulation could counteract the repressing effects of LBX2-AS1 silence on GC progression. Conclusion: In a word, LBX2-AS1 up-regulated by NFIC promoted GC progression via targeting miR-491-5p/ZNF703, implying LBX2-AS1 was an underlying treatment target for GC patients.

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“…KEGG pathway enrichment analyses revealed possible molecular pathways that might be altered in glaucoma patients. 59 However, none of these pathways is clearly known to be involved in glaucoma, and the mechanism for alleviated TM cell calcification in the ENST00000607393 knockdown cell model is unclear. Of interest, however, the mRNA expression levels of upstream components of the Wnt signaling pathway, Wnt, Frizzled, and Go/Gq, were significantly up-regulated in AH of glaucoma patients.…”

Section: Discussionmentioning

confidence: 99%

Potential Biomarkers for Primary Open-Angle Glaucoma Identified by Long Noncoding RNA Profiling in the Aqueous Humor

Xie

Mao

Wang

et al. 2019

The American Journal of Pathology

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This study aimed to identify potential biomarkers for primary open-angle glaucoma (POAG) diagnosis. First, long noncoding RNA (lncRNA) and mRNA expression profiles in the aqueous humor (AH) from 10 POAG and 10 control patients were accessed by microarray analyses. Coding-noncoding gene coexpression networks were drawn to predict potential lncRNA functions. lncRNA T267384, ENST00000607393, and T342877 expression levels were further tested by real-time quantitative PCR in AH from 29 POAG and 30 cataract patients, in iris tissues from 16 POAG patients and 10 controls, and in plasma from 49 POAG patients and 55 healthy controls. Finally, ENST00000607393 function was characterized in an in vitro model of cell calcification. A total of 3627 lncRNAs and 2228 mRNAs in the AH of POAG patients were significantly up-regulated, and 1520 lncRNAs and 820 mRNAs were significantly down-regulated. Seven lncRNAs showed positive correlation with glaucoma-associated gene, bone morphogenetic protein 2. Moreover, real-time quantitative RT-PCR confirmed that T267384, ENST00000607393, and T342877 expression levels were significantly higher in the AH from a different cohort of POAG patients. ENST00000607393 was also significantly higher in the iris and plasma of POAG patients. Last, ENST00000607393 knockdown alleviated calcification of primary human trabecular meshwork cells in vitro. Therefore, lncRNAs T267384, ENST00000607393, and T342877 may be potential biomarkers for POAG diagnosis. ENST00000607393 might be a new therapeutic target for trabecular meshwork calcification.

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Differentially expressed lncRNAs and miRNAs with associated ceRNA networks in aged mice with postoperative cognitive dysfunction

Cited by 51 publications

References 42 publications

MiR-190a potentially ameliorates postoperative cognitive dysfunction by regulating Tiam1

MiR-190a potentially ameliorates postoperative cognitive dysfunction by regulating Tiam1

LBX2-AS1 up-regulated by NFIC boosts cell proliferation, migration and invasion in gastric cancer through targeting miR-491-5p/ZNF703

Potential Biomarkers for Primary Open-Angle Glaucoma Identified by Long Noncoding RNA Profiling in the Aqueous Humor

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