Differential Thermal Analysis of Phospholipids

Chapman, D.; Collin, Delphine

doi:10.1038/206189a0

Cited by 52 publications

(17 citation statements)

References 4 publications

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“…at the gel-fluid transition temperature of DPPC. 42 When DPPC:Cer bilayers are examined ( Fig. 1) Cer is seen to enhance the mean free volume void size for most temperatures.…”

Section: Resultsmentioning

confidence: 95%

Ceramide increases free volume voids in DPPC membranes

et al. 2015

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Positron annihilation lifetime spectroscopy (PALS) can measure changes in local free volume voids in lipid bilayers. PALS has been applied, together with differential scanning calorimetry (DSC) and molecular dynamics (MD) simulations, to study free volume voids in DPPC and DPPC:ceramide (85:15 mol:mol) model membranes in the 20-60 ºC range. The free volume void average size clearly increases with the gel-fluid phase transition of the lipid, or lipid mixture. Ceramide increases void size at all temperatures, particularly in the range causing the gel-fluid transition of the mixture. A parallel study of PALS and calorimetric data indicates that, for the complex thermotropic transition of the DPPC:ceramide mixture, PALS is detecting the transition of the DPPC component, while calorimetry changes indicate mainly the melting of the ceramide-enriched domains. Molecular dynamics calculations provide a clear distinction between ceramide-rich and -poor domains, and show that the voids are predominantly located near the membrane nodal plane. The ceramide-induced increase in void volume size occurs as well at temperatures when both phospholipid and ceramide are in the fluid state, indicating that the effect is not the result of phospholipid-ceramide domain coexistence. The above observations may be related to hitherto unexplained properties of ceramide, such as the increase in membrane permeability, and the induction of transmembrane (flip-flop) lipid motion.

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“…at the gel-fluid transition temperature of DPPC. 42 When DPPC:Cer bilayers are examined ( Fig. 1) Cer is seen to enhance the mean free volume void size for most temperatures.…”

Section: Resultsmentioning

confidence: 95%

Ceramide increases free volume voids in DPPC membranes

et al. 2015

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“…The increased resistance to osmotic lysis of spheroplasts from cells grown at 15 C rather than 30 C, as the test temperature was lowered below 30 C, could be explained by the small increase in the proportion of unsaturated fatty acids in the spheroplast membranes from cells grown at the lower temperature. The presence of an increased proportion of unsaturation in lipids lowers the temperature at which the endothermic phase transition takes place (4,5,6). This phase transition is primarily associated with a "melting" of the hydrocarbon chains in the phospholipid, and the greater mobility in these chains in membranes from cells grown at the lower temperature could explain the greater resistance to osmotic lysis at low test temperatures.…”

Section: Discussionmentioning

confidence: 99%

Osmotic Properties of Spheroplasts fromSaccharomyces cerevisiaeGrown at Different Temperatures

Diamond

Rose

1970

J Bacteriol

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Spheroplasts were prepared from cells of Saccharomyces cerevisiae NCYC 366, grown at 30 or 15 C, by incubating cells with snail-gut juice after pretreatment with 2-mercaptoethanol. Walls of cells grown batchwise or in continuous culture at 15 C were more resistant to digestion with snail juice than walls on cells grown under the same conditions as 30 C. Spheroplasts lysed when suspended in hypotonic solutions of mannitol. The resistance of spheroplasts to osmotic lysis tended to increase when the test temperature was lowered below 30 C. The increased resistance was greater with spheroplasts from cells grown at 15 C. Cations, especially Ca 2+ , protected spheroplasts against osmotic lysis. In general, the protective effects, measured at 30 C, were smaller with spheroplasts from cells grown at 15 C compared with 30 C. Citrate and ethylenediaminetetraacetate (EDTA) decreased the resistance of spheroplasts to osmotic lysis. On the whole, the decrease was greater with spheroplasts from cells grown at 30 C rather than 15 C. In the presence of EDTA, spheroplasts from cells grown at 30 C were less resistant to osmotic lysis at 5 C than at 30 C; when spheroplasts from cells grown at 15 C were similarly examined, they were more resistant to lysis at 5 C than at 30 C. Spheroplast membranes from cells grown at 15 C had slightly but significantly greater contents of Mg 2+ , Ca 2+ , K + , and Na + compared with spheroplast membranes from cells grown at 15 C. Mg 2+ and Ca 2+ were more easily extracted with EDTA from membranes of 30 C-grown cells than from 15 C-grown cells.

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“…1) contained the highest phosphorus content of 4.72 ± 0.16 mg/kg (data not shown in Table 3). It is also possible that phosphorus adhered to the inner walls of the evaporator during the higher temperature processes [35], and solidified to the surfaces of the condenser, owing to the extremely high melting points of phospholipids (230-231 °C for certain phosphatidylcholines, which are one of the main phospholipid species in rice bran lipids) [7,36], while the temperature of the condenser was only 35-42 °C.…”

Section: Trace Substance Contentmentioning

confidence: 99%

Production of Rice Bran Oil with Light Color and High Oryzanol Content by Multi‐stage Molecular Distillation

Jin

Xie

Chen³

et al. 2015

J Americ Oil Chem Soc

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SRO-II and SRO-III had a clear yellow appearance and a non-triacylglycerol content <1.5 %. These semi-refined oils were mixed directly after MMD and further refined by mild deacidification and winterization to obtain fully refined oil, which met the requirements of commonly used standards. Notably, the final oil exhibited light color and retained nearly 80 % of the oryzanol of raw oil. The yield of final oil reached 80-85 % through the entire refining process.

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Differential Thermal Analysis of Phospholipids

Cited by 52 publications

References 4 publications

Ceramide increases free volume voids in DPPC membranes

Ceramide increases free volume voids in DPPC membranes

Osmotic Properties of Spheroplasts fromSaccharomyces cerevisiaeGrown at Different Temperatures

Production of Rice Bran Oil with Light Color and High Oryzanol Content by Multi‐stage Molecular Distillation

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