Differential roles for two gelatinolytic enzymes of the matrix metalloproteinase family in the remodelling cornea

Matsubara, Masahiko; Girard, M T; Kublin, Claire L.; Cintron, Charles; Fini, M. Elizabeth

doi:10.1016/0012-1606(91)90300-r

Cited by 214 publications

(163 citation statements)

References 39 publications

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“…4,5,7,9,11,12 Of relevance to this study, Azar et al 33 used zymography to compare MMP expression after PRK or LASIK in the rabbit cornea and found that stromal levels of MMP-2 and -9 increased after both procedures, with high MMP-9 expression also localised in the epithelium during re-epithelialisation. The role of MMP-1 was not examined.…”

Section: Discussionmentioning

confidence: 91%

“…[1][2][3] In the normal cornea, only very low levels of MMP-2 are found as proenzyme. [4][5][6] After injury, and in response to the release of cytokines, several MMPs in the cornea are upregulated by transcription or activation. 7 Preformed MMP-9 (Gelatinase B) may also be released from the secretory granules of neutrophils recruited by any associated inflammation.…”

Section: Introductionmentioning

confidence: 99%

“…8 It has been demonstrated that MMP-1 (interstitial collagenase), -2 (gelatinase A), -3 (stromelysin-1), and -9 participate in epithelial repair and stromal remodelling. 5,9 Their mechanism of action is uncertain, but MMP-1 is essential in vitro for the migration of corneal epithelial cells over a type I collagen matrix. 10 It has also been noted that the subepithelial expression of MMP-9 parallels basement membrane degredation, 11,12 while MMP-2 and -3 produced by stromal fibroblasts may mediate long-term stromal remodelling and basement membrane synthesis.…”

Section: Introductionmentioning

confidence: 99%

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Matrix metalloproteinase distribution during early corneal wound healing

Mulholland¹,

Tuft²,

Khaw³

2004

Eye

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Aim To compare matrix metalloproteinase (MMP) localisation in anterior keratectomy (AK) and lamellar keratectomy (LK) wounds. Methods Wounds were produced in one eye of 24 rabbits. The AK wounds were made to approximately 120 mm in depth and then allowed to re-epithelialise. The LK wounds were of similar depth, but the anterior stroma and epithelium were replaced after a second deeper keratectomy had been performed. Immunohistochemistry was used to localise the MMP-1, -2, -3, and -9 at intervals from 4 h to 14 days following surgery. The contralateral eyes acted as controls.Results After an AK wound MMP-1 was present at the leading edge of migrating epithelium after 18 h, while MMP-2 and -9 were localised behind the advancing epithelial edge. The presence of these enzymes rapidly fell to low levels after epithelial closure. There was only faint MMP-3 localisation between days 3 and 7. After an LK wound, MMP-1, -3, and -9 were not detected in the stromal interface, but MMP-2 was present at all time points. Conclusions This study suggests that after an AK wound, MMP-1 is a key mediator of epithelial migration, while MMP-2 and -9, and to a lesser extent MMP-3, may participate in the remodelling of corneal stroma and the reformation of epithelial basement membrane. In contrast, an LK wound results in a much lower stimulus for MMP activation. The action of MMP-2 in stromal repair is thus partly independent of epithelial injury.

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Matrix metalloproteinase distribution during early corneal wound healing

Mulholland¹,

Tuft²,

Khaw³

2004

Eye

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“…3). A expressão de MMP9 é associada à degradação da membrana basal e o seu desaparecimento coincide com a reabsorção da matriz provisória e com a restauração dos complexos de adesão (Matsubara et al, 1991). As metaloproteinases de matriz tipo membrana (MT-MMPs) estão associadas às membranas celulares e têm funções importantes na sinalização celular e proteólises pericelulares.…”

Section: Resultsunclassified

Expressão de metaloproteinases de matriz e PCNA em úlceras de córnea profundas, induzidas em coelhos, tratadas com plasma rico em plaquetas

Perches

Pellizzon

Ranzani

et al. 2015

Arq. Bras. Med. Vet. Zootec.

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“…Gel Zymography-The presence of specific MMP protein species in retinal homogenates was determined by zymography (74) according to our standard lab protocol (46). This technique detects both latent proenzymes and active enzyme forms based on the capacity for activation of proenzymes by SDS.…”

Section: Methodsmentioning

confidence: 99%

Deficiency in Matrix Metalloproteinase Gelatinase B (MMP-9) Protects against Retinal Ganglion Cell Death after Optic Nerve Ligation

Chintala

Zhang

Austin

et al. 2002

Journal of Biological Chemistry

129

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Loss of retinal ganglion cells is the final end point in blinding diseases of the optic nerve such as glaucoma.To enable the use of mouse genetics to investigate mechanisms underlying ganglion cell loss, we adapted an experimental model of optic nerve ligation to the mouse and further characterized post-surgical outcome. We made the novel finding that apoptosis of retinal ganglion cells correlates with specific degradation of laminin from the underlying inner limiting membrane and an increase in gelatinolytic metalloproteinase activity. These changes co-localize with a specific increase in levels of the matrix metalloproteinase, gelatinase B (GelB; MMP-9). Using a transgenic mouse line harboring a reporter gene driven by the GelB promoter, we further show that increased GelB is controlled by activation of the GelB promoter. These findings led us to hypothesize that GelB activity plays a role in ganglion cell death and degradation of laminin. Applying the genetic approach, we demonstrate that GelB-deficient mice are protected against these pathological changes. This is the first report demonstrating a causal connection between GelB activity and pathological changes to the inner retina after optic nerve ligation.

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Differential roles for two gelatinolytic enzymes of the matrix metalloproteinase family in the remodelling cornea

Cited by 214 publications

References 39 publications

Matrix metalloproteinase distribution during early corneal wound healing

Matrix metalloproteinase distribution during early corneal wound healing

Expressão de metaloproteinases de matriz e PCNA em úlceras de córnea profundas, induzidas em coelhos, tratadas com plasma rico em plaquetas

Deficiency in Matrix Metalloproteinase Gelatinase B (MMP-9) Protects against Retinal Ganglion Cell Death after Optic Nerve Ligation

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