Differential Requirement for Translation Initiation Factor Pathways during Ecdysone-Dependent Neuronal Remodeling in Drosophila

Rode, Sandra; Ohm, Henrike; Anhäuser, Lea; Wagner, Marina; Rosing, Mechthild; Deng, Xiaobing; Sin, Olga; Leidel, Sebastian A.; Storkebaum, Erik; Rentmeister, Andrea; Zhu, Sijun; Rumpf, Sebastian

doi:10.1016/j.celrep.2018.07.074

Cited by 40 publications

(46 citation statements)

References 55 publications

(97 reference statements)

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“…Indeed, if eIF3d interaction with the cap was involved, overexpression of the point mutant eIF3d helix11 that is unable to bind the cap, 41 would be expected to induce negative dominant defects, due to the lack of translation mediated by this interaction. 49 However, overexpression of eIF3d helix11 with the nos-Gal4 driver did not induce any defects in embryonic development or Nos protein synthesis (Supplementary information, Fig. S6).…”

Section: Discussionmentioning

confidence: 94%

“…41 (3) In the same line, eIF3d was involved in cap-dependent translational activation of specific mRNAs for neuronal remodeling in Drosophila larvae, in a context where eIF4E is blocked by 4E-binding protein (4E-BP). 49 Other studies have reported the role of eIF3 in promoting cap-independent translation, thus highlighting eIF3 functional versatility in the control of translation. eIF3 was shown to directly bind methylated adenosine m 6 A, in mRNA 5′UTRs to induce cap-independent translation under stress conditions.…”

Section: Discussionmentioning

confidence: 97%

“…71 Cloning and recombineering To produce the UASp-HA-eIF3d and UASp-HA-eIF3d helix11 transgenes, eIF3d and eIF3d helix11 coding sequences were amplified by PCR from clones provided by S. Rumpf. 49 PCR fragments were cloned into pENTR by directional Topo cloning (Invitrogen). The generated plasmids were used in gateway cloning to insert the sequences into pPHW (UASp-HA-attR1-ccdB-attR2-SV40 3′UTR) in which an attB (pPHW-attB) site has been inserted.…”

Section: Polysome Profilingmentioning

confidence: 99%

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The PIWI protein Aubergine recruits eIF3 to activate translation in the germ plasm

et al. 2020

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Piwi-interacting RNAs (piRNAs) and PIWI proteins are essential in germ cells to repress transposons and regulate mRNAs. In Drosophila, piRNAs bound to the PIWI protein Aubergine (Aub) are transferred maternally to the embryo and regulate maternal mRNA stability through two opposite roles. They target mRNAs by incomplete base pairing, leading to their destabilization in the soma and stabilization in the germ plasm. Here, we report a function of Aub in translation. Aub is required for translational activation of nanos mRNA, a key determinant of the germ plasm. Aub physically interacts with the poly(A)-binding protein (PABP) and the translation initiation factor eIF3. Polysome gradient profiling reveals the role of Aub at the initiation step of translation. In the germ plasm, PABP and eIF3d assemble in foci that surround Aub-containing germ granules, and Aub acts with eIF3d to promote nanos translation. These results identify translational activation as a new mode of mRNA regulation by Aub, highlighting the versatility of PIWI proteins in mRNA regulation.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 97%

Section: Polysome Profilingmentioning

confidence: 99%

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The PIWI protein Aubergine recruits eIF3 to activate translation in the germ plasm

et al. 2020

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“…Thus, our data suggest that eIF6 is upstream of ecdysone regulation. It has been recently suggested how translation regulation and hormonal signaling are tightly interconnected in Drosophila 40 and, more generally, that translation is a controller of metabolism 41 . Our experiments unveil an inverse correlation between translational capability and ecdysone production.…”

Section: Discussionmentioning

confidence: 99%

The eukaryotic Initiation Factor 6 (eIF6) regulates ecdysone biosynthesis by modulating translation inDrosophila

Russo

Gatti

Alfieri

et al. 2017

Preprint

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22Increases in ribosomal proteins and initiation factors are often observed in tumours 23 and required during development. Haploinsufficient models have shown that such 24 elevation is essential for tumour growth. Models with increased gene dosage of 25 initiation factors, addressing the effects of their forced overexpression are lacking. 26The eukaryotic Initiation Factor 6 (eIF6) gene is amplified in some cancers and

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“…mRNA translated by ribosomes is capped at the 5 end, and the cap requires recruitment of eukaryotic initiation factors (eIFs) such as eIF4E and eIF4G before they, in turn, recruit the ribosome (Ostroff et al, 2017;Choi et al, 2018;Das Sharma et al, 2019). Some eIFs are even preferentially involved in different stages of dendritic growth and maintenance, such as eIF4A and eIF3, which have been found to be required for dendritic pruning in Drosophila pupae (Rode et al, 2018). Additionally, some ribosomes can directly bind with mRNA using Internal Ribosome Entry Sites (IRES); however, even while skipping the "middle man", they still depend on a whole host of other proteins that assist with initiation, elongation, and termination of the mRNA (Sutton and Schuman, 2005;Genuth and Barna, 2018).…”

Section: Protein Synthesis and Plasticitymentioning

confidence: 99%

Homeostatic Roles of the Proteostasis Network in Dendrites

Lottes

Cox

2020

Front. Cell. Neurosci.

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Cellular protein homeostasis, or proteostasis, is indispensable to the survival and function of all cells. Distinct from other cell types, neurons are long-lived, exhibiting architecturally complex and diverse multipolar projection morphologies that can span great distances. These properties present unique demands on proteostatic machinery to dynamically regulate the neuronal proteome in both space and time. Proteostasis is regulated by a distributed network of cellular processes, the proteostasis network (PN), which ensures precise control of protein synthesis, native conformational folding and maintenance, and protein turnover and degradation, collectively safeguarding proteome integrity both under homeostatic conditions and in the contexts of cellular stress, aging, and disease. Dendrites are equipped with distributed cellular machinery for protein synthesis and turnover, including dendritically trafficked ribosomes, chaperones, and autophagosomes. The PN can be subdivided into an adaptive network of three major functional pathways that synergistically govern protein quality control through the action of (1) protein synthesis machinery; (2) maintenance mechanisms including molecular chaperones involved in protein folding; and (3) degradative pathways (e.g., Ubiquitin-Proteasome System (UPS), endolysosomal pathway, and autophagy. Perturbations in any of the three arms of proteostasis can have dramatic effects on neurons, especially on their dendrites, which require tightly controlled homeostasis for proper development and maintenance. Moreover, the critical importance of the PN as a cell surveillance system against protein dyshomeostasis has been highlighted by extensive work demonstrating that the aggregation and/or failure to clear aggregated proteins figures centrally in many neurological disorders. While these studies demonstrate the relevance of derangements in proteostasis to human neurological disease, here we mainly review recent literature on homeostatic developmental roles the PN machinery plays in the establishment, maintenance, and plasticity of stable and dynamic dendritic arbors. Beyond basic housekeeping functions, we consider roles of PN machinery in protein quality control mechanisms linked to dendritic plasticity (e.g., dendritic spine remodeling during LTP); cell-type specificity; dendritic morphogenesis; and dendritic pruning.

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Differential Requirement for Translation Initiation Factor Pathways during Ecdysone-Dependent Neuronal Remodeling in Drosophila

Cited by 40 publications

References 55 publications

The PIWI protein Aubergine recruits eIF3 to activate translation in the germ plasm

The PIWI protein Aubergine recruits eIF3 to activate translation in the germ plasm

The eukaryotic Initiation Factor 6 (eIF6) regulates ecdysone biosynthesis by modulating translation inDrosophila

Homeostatic Roles of the Proteostasis Network in Dendrites

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