Differential proteomic expression in indolent vulvar lichen sclerosus, transforming vulvar lichen sclerosus and normal vulvar tissue

Gleue, Casey A.; Xie, Fangyi; Deschaine, Maria; Dasari, Surendra; Sartori‐Valinotti, Julio C.; Torgerson, Rochelle R.; Davis, Mark D. P.; Charlesworth, M. Cristine; Meves, Alexander; Lehman, Julia S.

doi:10.1111/exd.14660

Cited by 4 publications

(9 citation statements)

References 23 publications

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“…We used formalin‐fixed paraffin‐embedded (FFPE) oral mucosal biopsies from the aforementioned patients for mass spectrometry. We utilized a previously published method for extracting the proteins and subjecting them to proteomics analysis 11,12 . In brief, 10‐μm‐thick sections were obtained for each patient and stained with haematoxylin‐and‐eosin (H&E).…”

Section: Methodsmentioning

confidence: 99%

“…We utilized a previously published method for extracting the proteins and subjecting them to proteomics analysis. 11,12 In brief, 10μm-thick sections were obtained for each patient and stained with haematoxylin-and-eosin (H&E). Unstained sections of the same thickness were mounted on polyethylene naphthalate (PEN) membrane slides for laser pressure catapulting to collect tissue (Zeiss Microbeam).…”

Section: F I G U R Ementioning

confidence: 99%

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Differential proteomic expression in indolent versus transforming oral lichen planus

Xie

Gleue

Deschaine

et al. 2023

Experimental Dermatology

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Oral lichen planus (OLP) confers an approximately 1% risk of transformation to oral squamous cell carcinoma (OSCC). Early identification of high‐risk OLP would be very helpful for optimal patient management. We aimed to discover specific tissue‐based protein biomarkers in patients with OLP who developed OSCC compared to those who did not. We used laser capture microdissection– and nanoLC–tandem mass spectrometry to assess protein expression in fixed lesional mucosal specimens in patients with indolent OLP (no OSCC after at least 5‐year follow‐up, n = 6), transforming OLP (non‐dysplastic epithelium with lichenoid inflammation marginal to OSCC, n = 6) or normal oral mucosa (NOM, n = 5). Transforming OLP protein profile was enriched for actin cytoskeleton, mitochondrial dysfunction and oxidative phosphorylation pathways. CA1, TNNT3, SYNM and MB were overexpressed, and FBLN1 was underexpressed in transforming OLP compared with indolent OLP. Integrin signalling and antigen presentation pathways were enriched in both indolent and transforming OLP compared with NOM. This proteomic study provides potential biomarkers, such as CA1 overexpression, for higher‐risk OLP. While further validation studies are needed, we propose that epithelial–mesenchymal transition may be involved in OLP carcinogenesis.

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Section: Methodsmentioning

confidence: 99%

Section: F I G U R Ementioning

confidence: 99%

Differential proteomic expression in indolent versus transforming oral lichen planus

Xie

Gleue

Deschaine

et al. 2023

Experimental Dermatology

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“…The blood supply can be improved and nutrition of the skin can be increased in the local nonneoplastic lesions of vulvar epithelium ( 17 , 18 ). Local scratching-damaged tissue can be repaired to maintain the integrity and elasticity of vulvar skin ( 19 ). In our study, we found that both treatments were clinically more effective for both types of lesions; the overall efficacy at 1, 3, and 6 months after treatment was above 50%, with the laser treatment group experiencing the most pronounced effect.…”

Section: Discussionmentioning

confidence: 99%

A retrospective comparative cohort study of ultra-pulse CO2 lattice laser and glucocorticoids in the treatment of vulvar epithelial nonneoplastic lesions

Wei¹,

Li²,

Zhang³

et al. 2023

Ann Transl Med

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Background: A comparison of topical glucocorticoids with CO 2 fractional laser treatment was conducted to investigate the differences in the efficacy of non-neoplastic vulvar epithelial lesion treatments in different pathological types and to provide a scientific basis for the management of these disorders. This paper was to study the difference of curative effect of different pathological types of non-tumor vulvar epithelial lesions and provide scientific basis for the treatment of these diseases. Methods:From November 2016 to July 2018, 178 cases of vulvar lichen simplex chronicus (LSC) or lichen sclerosus were confirmed with vulvar biopsy at our institute. Finally, 160 patients were enrolled in this trial. The patients were divided into 2 groups: a group treated with topical hormone and a group treated with CO 2 lattice laser therapies. There were 80 cases in each group, including 40 with LSC and 40 with lichen sclerosus. Patients applied 1 gram of progesterone cream and betamethasone cream to the affected area in the morning and evening, respectively, once a day for 3 months. The efficacy was evaluated with the Patient Global Impression of Change (PGI-C) subjective symptom improvement scale and clinical efficacy evaluation scale. The formula was applied to calculate the curative effect index. Results:The PGI-C scores at 1, 3, and 6 months of treatment showed that the laser treatment group had remarkably superior outcomes to the glucocorticoid treatment group. The clinical efficacy score scale at 3and 6-month treatments indicated a significantly greater curative effect in the laser than in the glucocorticoid treatment (P=0.006 and P=0.002 respectively). In the glucocorticoid group, the clinical effects of different pathological subtypes were significantly different following the 1-and 3-month treatments. The efficacy of treatment for LSC was better than that for lichen sclerosus. Following the 3-and 6-month treatments, the clinical effect for LSC was better than that of lichen sclerosus (3 months: 95% vs. 75%; 6 months: and 95% vs. 70%).Conclusions: Ultrapulse CO 2 lattice laser was more effective than was glucocorticoid therapy in the treatment of vulvar epithelial non-tumor-like lesions.

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“…Proteomic profiles of these NVT and VLS samples were previously published by our team in a study examining malignant transformation in VLS. 12 OLP and NOM samples were also previously published in a study of carcinogenesis in OLP. 13 There were insufficient VLP samples associated with development of vulvar SCC to address the question of malignant transformation for VLP.…”

Section: Patient Selectionmentioning

confidence: 99%

“…We utilized a previously published method for extracting the proteins and subjecting them to proteomics analysis. 12,13,17 In brief, 10 μm-thick sections were obtained for each patient and stained with haematoxylin-and-eosin (H&E). Unstained sections of the same thickness were mounted on polyethylene naphthalate (PEN) membrane slides for laser pressure catapulting to collect tissue (Zeiss Microbeam).…”

Section: Tissue Collectionmentioning

confidence: 99%

Differential proteomic expression profiles in vulvar lichen planus as compared to normal vulvar tissue, vulvar lichen sclerosus, or oral lichen planus: An exploratory study

Xie

Dasari

Deschaine

et al. 2023

Experimental Dermatology

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Vulvar lichen planus (VLP) is a chronic inflammatory disease which adversely affects patients' quality of life. The pathogenesis of VLP is unknown although Th1 immune response has been implicated. We aimed to discover specific tissue‐based protein biomarkers in VLP compared to normal vulvar tissue (NVT), vulvar lichen sclerosus (VLS) and oral lichen planus (OLP). We used laser capture microdissection‐liquid chromatography‐ tandem mass spectrometry to assess protein expression in fixed lesional mucosal specimens from patients with VLP (n = 5). We then compared proteomic profiles against those of NVT (n = 4), VLS (n = 5), OLP (n = 6) and normal oral mucosa (n = 5), previously published by our group. IL16, PTPRC, PTPRCAP, TAP1 and ITGB2 and were significantly overexpressed in VLP compared to NVT. Ingenuity pathway analysis identified antigen presentation and integrin signalling pathways. Proteins overexpressed in both VLP versus NVT and OLP versus NOM included IL16, PTPRC, PTPRCAP, TAP1, HLA‐DPB1, HLA‐B and HLA‐DRA. This proteomic analysis revealed several overexpressed proteins in VLP that relate to Th1 autoimmunity, including IL16. Overlapping pathways, including those involving IFNγ and Th1 signalling, were observed between VLP, VLS, and OLP.

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Differential proteomic expression in indolent vulvar lichen sclerosus, transforming vulvar lichen sclerosus and normal vulvar tissue

Cited by 4 publications

References 23 publications

Differential proteomic expression in indolent versus transforming oral lichen planus

Differential proteomic expression in indolent versus transforming oral lichen planus

A retrospective comparative cohort study of ultra-pulse CO2 lattice laser and glucocorticoids in the treatment of vulvar epithelial nonneoplastic lesions

Differential proteomic expression profiles in vulvar lichen planus as compared to normal vulvar tissue, vulvar lichen sclerosus, or oral lichen planus: An exploratory study

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