Differential proteomic analysis to identify potential biomarkers associated with quality traits of Dezhou donkey meat using a data-independent acquisition (DIA) strategy

Chai, Wenqiong; Xu, Jing; Qu, Honglei; Ma, Qiugang; Zhu, Mingxia; Li, Mengmeng; Zhan, Yandong; Wang, Tianqi; Gao, Jingrong; Yao, Huanfen; Li, Zeyu; Wang, Changfa

doi:10.1016/j.lwt.2022.113792

Cited by 9 publications

(7 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The identified proteins were found to be associated with various biological processes, including metabolic processes, cellular processes, and biological regulation. Additionally, several proteins were identified that were associated with meat quality traits such as tenderness, water-holding capacity, and juiciness [33]. Similarly, another study investigated the heterogeneity of intramuscular fat (IMF) and visceral adipose tissue (VAT) in Dezhou donkeys using lipidomics and transcriptomics profiling [67].…”

Section: Transcriptomic and Whole-genome Sequencing Analysis For The ...mentioning

confidence: 99%

“…For instance, donkey milk is believed to possess properties such as antioxidants, antimicrobials, antiproliferatives, and antidiabetic agents, which may have implications for treating a range of diseases [27][28][29][30][31]. Thus, a growing interest has been documented in studying genetic markers associated with production traits in donkeys in order to provide valuable insights into how to improve their productivity and usefulness for various purposes [32][33][34][35][36][37]. For the screening of genetic markers associated with production traits, various approaches, including genome-wide association studies for the identification of QTLs, SNPs, InDel, direct marker selection, and transcriptomic analysis, have been widely utilized in animals.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Exploring Genetic Markers: Mitochondrial DNA and Genomic Screening for Biodiversity and Production Traits in Donkeys

Huang,

Khan,

Chai

et al. 2023

Animals

View full text Add to dashboard Cite

Donkeys (Equus asinus) play a pivotal role as essential livestock in arid and semi-arid regions, serving various purposes such as transportation, agriculture, and milk production. Despite their significance, donkey breeding has often been overlooked in comparison to other livestock species, resulting in limited genetic improvement programs. Preserving donkey genetic resources within each country necessitates the establishment of breed conservation programs, focusing on managing genetic diversity among populations. In recent years, significant strides have been made in sequencing and analyzing complete mitochondrial DNA (mtDNA) molecules in donkeys. Notably, numerous studies have honed in on the mitochondrial D-loop region, renowned for its remarkable variability and higher substitution rate within the mtDNA genome, rendering it an effective genetic marker for assessing genetic diversity in donkeys. Furthermore, genetic markers at the RNA/DNA level have emerged as indispensable tools for enhancing production and reproduction traits in donkeys. Traditional animal breeding approaches based solely on phenotypic traits, such as milk yields, weight, and height, are influenced by both genetic and environmental factors. To overcome these challenges, genetic markers, such as polymorphisms, InDel, or entire gene sequences associated with desirable traits in animals, have achieved widespread usage in animal breeding practices. These markers have proven increasingly valuable for facilitating the selection of productive and reproductive traits in donkeys. This comprehensive review examines the cutting-edge research on mitochondrial DNA as a tool for assessing donkey biodiversity. Additionally, it highlights the role of genetic markers at the DNA/RNA level, enabling the informed selection of optimal production and reproductive traits in donkeys, thereby driving advancements in donkey genetic conservation and breeding programs.

show abstract

Section: Transcriptomic and Whole-genome Sequencing Analysis For The ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Exploring Genetic Markers: Mitochondrial DNA and Genomic Screening for Biodiversity and Production Traits in Donkeys

Huang,

Khan,

Chai

et al. 2023

Animals

View full text Add to dashboard Cite

show abstract

“…Donkeys have primarily been utilized as agricultural labor for thousands of years. In recent years, with the modernization of agriculture, donkeys have emerged as multi-purpose farm creatures in some countries, such as companion animals, and even dairy or meat-producing animals [ 30 ]. Our previous toxicokinetics research of ochratoxin and deoxynivalenol in donkeys showed the absorption, elimination, and excretion parameters of both toxins in donkeys were different from other animals [ 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

Toxicokinetics of Zearalenone following Oral Administration in Female Dezhou Donkeys

Qu,

Zheng,

Kang

et al. 2024

Toxins

Self Cite

View full text Add to dashboard Cite

Zearalenone (ZEN) is a mycotoxin produced by various Fusarium strains, that is present in food and feed raw materials worldwide, causing toxicity effects in animals and humans. This research aimed to explore the toxicokinetics of ZEN on female Dezhou donkeys following a single oral exposure dosage of 2 mg/kg BW (body weight). The sample collection of donkeys plasma was carried out at 0, 5, 10, 15, 20, 30, 45, 60, 90 min, 2 h, 2.5 h, 3 h, 3.5 h, 4 h, 4.5 h, 6 h, 9 h, 12 h, 24 h, 48 h, 72 h, 96 h and 120 h via intravenous catheter, and fecal and urinary samples were severally collected at 0 h and every 6 h until 120 h. The concentrations of ZEN, α-zearalenol (α-ZOL), β-zearalenol (β-ZOL), α-zearalanol (α-ZAL), β-zearalanol (β-ZAL), zearalanone (ZAN) in plasma, urine, and feces were detected by UPLC-MS/MS. Only ZEN was detected in plasma, and the maximum was 15.34 ± 5.12 µg/L occurred at 0.48 h after gavage. The total plasma clearance (Cl) of ZEN was 95.20 ± 8.01 L·kg·BW−1·h−1. In addition, the volume of distribution (Vd) was up to 216.17 ± 58.71 L/kg. The percentage of total ZEN (ZEN plus the main metabolites) excretion in feces and urine was 2.49% and 2.10%, respectively. In summary, ZEN was fast absorbed and relatively slowly excreted in female donkeys during 120 h after a single gavage, indicating a trend of wider tissue distribution and longer tissue persistence.

show abstract

“…Dezhou donkey (Fig. 1 ) is one of the five best donkey breeds in China, with a distinctive feature of tall size, fast growth rate, good production characteristics and stable genetic performance 1 , 2 . In 2011, Dezhou donkeys were included in the national livestock and poultry genetic resources protection list.…”

Section: Introductionmentioning

confidence: 99%

“…After careful cultivation, it has gradually formed a unique local breed. With the development of agricultural mechanization, donkeys had gradually lost their traditional role as working animals 3 and become dual-purpose farm animals 1 . Statistics from China’s Statistical Yearbook showed that the number of donkeys dropped from 9.444 million in 1994 to 2.324 million in 2020.…”

Section: Introductionmentioning

confidence: 99%

An investigation of genetic diversity in three Dezhou donkey original breeding farms

Wang

Liu

Shi

et al. 2023

Sci Rep

Self Cite

View full text Add to dashboard Cite

Dezhou donkey is one of the excellent large donkey breeds in China. In our study, eight microsatellite markers were used to genotype from each of three populations of Chinese Dezhou donkeys: 67 individuals in Liaocheng (pop1), 103 individuals in Binzhou 1 (pop2), and 102 individuals in Binzhou 2 (pop3), in order to study the genetic diversity of these populations. A total of 213 alleles were detected, and the PIC results showed that eight loci were all highly polymorphic. The means of Ho and He of pop2 were the highest, which were 0.646 and 0.717, respectively. The PCoA analysis demonstrated that the samples from three conservation farms were mixed together. The phylogenetic tree showed that pop 2 and pop 3 were closely related. The phylogenetic tree results clustered that 272 donkeys were divided into six groups. AMOVA analysis showed that the genetic variation was mainly concentrated within the population and the genetic differentiation among the populations was low. Fst values between populations also indicated that genetic differentiation between populations was too small to be considered. This indicated a low probability of inbreeding within the population. And this also showed that the conservation and breeding of Dezhou donkeys in recent years have achieved excellent results. The investigation of genetic diversity in three Dezhou donkey original breeding farms can provide reference data for the selection and breeding of excellent breeds of Dezhou donkey.

show abstract

Differential proteomic analysis to identify potential biomarkers associated with quality traits of Dezhou donkey meat using a data-independent acquisition (DIA) strategy

Cited by 9 publications

References 39 publications

Exploring Genetic Markers: Mitochondrial DNA and Genomic Screening for Biodiversity and Production Traits in Donkeys

Exploring Genetic Markers: Mitochondrial DNA and Genomic Screening for Biodiversity and Production Traits in Donkeys

Toxicokinetics of Zearalenone following Oral Administration in Female Dezhou Donkeys

An investigation of genetic diversity in three Dezhou donkey original breeding farms

Contact Info

Product

Resources

About