Differential localization of high‐ and low‐molecular‐weight variants of microtubule‐associated protein 2 in the developing rat telencephalon

Fujimori, Kazuhiro E.; Takauji, Rumiko; Tamamaki, Nobuaki

doi:10.1002/cne.10286

Cited by 13 publications

(12 citation statements)

References 41 publications

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“…The monopolar or bipolar morphology of pioneering cortical MAP2-positive neurons is typical of tangentially migrating cells (Tamamaki et al, 1997;Fujimori et al, 2002). The fact that we found similar cells at the level of the ventricular angle and in the intermediate zone of the basal telencephalon (Fig.…”

Section: Pioneer Neurons Of the Human Cortexmentioning

confidence: 49%

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Tangential Networks of Precocious Neurons and Early Axonal Outgrowth in the Embryonic Human Forebrain

Bystron¹,

Molnár²,

Otellin³

et al. 2005

J. Neurosci.

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We used a combination of immunohistochemistry and carbocyanine dye tracing to study neurons and their processes in the human embryonic forebrain, 4 -7 weeks after conception, before the onset of synaptogenesis. We discovered a widespread network of precocious MAP2 (microtubule-associated protein 2)-immunoreactive cells, with long, nonaxonal processes, before the appearance of the cortical plate and the establishment of thalamocortical connectivity. Dye tracing revealed that the processes of these precocious cells form tangential links between intermediate zones of the thalamus, ganglionic eminence, hypothalamus, and cortical preplate. The spatiotemporal distribution and morphology of the precocious neurons in the cortical preplate suggest that they are generated outside the cerebral wall rather than in the local ventricular zone. The first thalamocortical axons and axons of preplate cells extend across diencephalotelencephalic and striatocortical boundaries before the arrival of the first cortical plate neurons. Precocious cells may provide initial communication between subdivisions of the embryonic brain as well as guidance cues for navigation of growing axons and/or transverse neuronal migration.

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Section: Pioneer Neurons Of the Human Cortexmentioning

confidence: 49%

“…MAP2 occurs in developing mammalian neuronal tissue as both high-and low-molecular weight forms, with temporally regulated expression (Fujimori et al, 2002). It is much more abundant in cell bodies and dendrites than in axons.…”

Section: Methodsmentioning

confidence: 99%

Tangential Networks of Precocious Neurons and Early Axonal Outgrowth in the Embryonic Human Forebrain

Bystron¹,

Molnár²,

Otellin³

et al. 2005

J. Neurosci.

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“…To test this, we performed double-labeling studies of Dlx2 and GABA immunohistochemistry (Fig. 1R) and Dlx2 and MAP2 immunohistochemistry (Fujimori et al, 2002) extensively on the frontal sections of E16 embryo brains and found that more than 88% of the Dlx2-positive cells were neurons. Dlx2-positive oligodendrocyte precursors thus appear to make up less than 12% of Dlx2-positive cells migrating from the GE to the neocortex in the IZ of embryos at E16.…”

Section: Resultsmentioning

confidence: 98%

Evidence that Sema3A and Sema3F regulate the migration of GABAergic neurons in the developing neocortex

Tamamaki

Fujimori

Nojyo

et al. 2002

J of Comparative Neurology

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103

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The ganglionic eminence (GE) supplies neurons containing gamma-aminobutyric acid (GABA) to the pallium of the telencephalon. We investigated the molecular guidance mechanisms of GE cell migration in the neocortex and found neuropilin-1 (Npn-1) or neuropilin-2 (Npn-2) on the GE cells. Ectopic Sema3A or -3F expression by COS1 cell clusters placed on embryo neocortical slices reduced the cell migration but did not block it completely. However, the cell migration was almost completely blocked by COS1 cell clusters expressing both Sema3A and -3F. The direction of cell migration could be reversed by placing Sema3A- and -3F-coexpressing COS1 cell clusters at the distal cut end of the neocortical slices. Further slice experiments revealed that migration of half of the GE cells in the neocortex was regulated by Sema3A and that migration of the other half of the GE cells in the neocortex was regulated by Sema3F. When the cells responding to Sema3A were diverted by ectopic Sema3A expression in vivo, Dlx2-positive cells were found predominantly in the lower intermediate zone (IZ). When the cells responding to Sema3F were diverted by ectopic Sema3F expression in vivo, Dlx2-positive cells were found predominantly in the upper IZ. It was speculated that the semaphorin-neuropilin interactions distribute the GABAergic GE cells evenly in the neocortex as well as guide the GE cells from the GE to the neocortex. The Sema3A expression site under the subplate extended dorsally as the embryo developed. The Sema3A expression seemed to block the Npn-1-positive GE cells in the neocortex from entering the cortical plate (CP) and guide them to the dorsal cortex and the hippocampus. Sema3F expression in the CP continued through the embryonic stages. The expression seemed to block Npn-2-positive GE cells in the neocortex from entering the CP and make them migrate into the lower IZ. Finally, the semaphorin-neuropilin interactions sorted GABAergic inteneurons into the CP and white matter neurons into the IZ.

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“…To test this, we performed double‐labeling studies of Dlx2 and GABA immunohistochemistry (Fig. 1R) and Dlx2 and MAP2 immunohistochemistry (Fujimori et al, 2002) extensively on the frontal sections of E16 embryo brains and found that more than 88% of the Dlx2‐positive cells were neurons. Dlx2‐positive oligodendrocyte precursors thus appear to make up less than 12% of Dlx2‐positive cells migrating from the GE to the neocortex in the IZ of embryos at E16.…”

Section: Resultsmentioning

confidence: 99%

Hormonal effects of Depo-Provera in cervical smears

Kaptain

Bloom

2002

Cancer

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Differential localization of high‐ and low‐molecular‐weight variants of microtubule‐associated protein 2 in the developing rat telencephalon

Cited by 13 publications

References 41 publications

Tangential Networks of Precocious Neurons and Early Axonal Outgrowth in the Embryonic Human Forebrain

Tangential Networks of Precocious Neurons and Early Axonal Outgrowth in the Embryonic Human Forebrain

Evidence that Sema3A and Sema3F regulate the migration of GABAergic neurons in the developing neocortex

Hormonal effects of Depo-Provera in cervical smears

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