Differential induction of ornithine decarboxylase (ODC) gene family members in transgenic tobacco (Nicotiana tabacum L. cv. Bright Yellow 2) cell suspensions by methyl-jasmonate treatment

Xu, Bingfang; Sheehan, Moira; Timko, Michael P.

doi:10.1023/b:grow.0000049419.22779.f5

Cited by 37 publications

(20 citation statements)

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“…This gene was induced in a 5‐day culture of cell lines overexpressing NtMYBJS1 (Table S6), which overproduce phenylpropanoid substrates in the cells. In contrast, the alternative ODC gene on the array ( BP526041 ) encodes a novel tobacco ODC protein, dissimilar to the proteins summarized by Xu et al. (2004), yet similar to the putative ODC from Solanum demissum .…”

Section: Discussionmentioning

confidence: 94%

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A novel R2R3 MYB transcription factor NtMYBJS1 is a methyl jasmonate‐dependent regulator of phenylpropanoid‐conjugate biosynthesis in tobacco

Šimek²,

et al. 2006

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SummaryTarget metabolic and large-scale transcriptomic analyses of tobacco (Nicotiana tabacum L.) Bright Yellow-2 (BY-2) cells were employed to identify novel gene(s) involved in methyl jasmonate (MJ)-dependent function in plants. At the metabolic level, we describe the specific accumulation of several phenylpropanoid-polyamine conjugates in MJ-treated BY-2 cells. Furthermore, global gene expression analysis of MJ-treated cells using a 16K cDNA microarray containing expressed sequence tags (ESTs) from BY-2 cells revealed 828 genes that were upregulated by MJ treatment within 48 h. Using time-course expression data we identified a novel MJinducible R2R3 MYB-type transcription factor (NtMYBJS1) that was co-expressed in a close temporal pattern with the core phenylpropanoid genes phenylalanine ammonia-lyase (PAL) and 4-coumarate:CoA ligase (4CL). Overexpression of NtMYBJS1 in tobacco BY-2 cells caused accumulation of specific phenylpropanoid conjugates in the cells. Subsequent microarray analysis of NtMYBJS1 transgenic lines revealed that a limited number of genes, including PAL and 4CL, were specifically induced in the presence of the NtMYBJS1 transgene. These results, together with results of both antisense expression analysis and of gel mobility shift assays, strongly indicate that the NtMYBJS1 protein functions in tobacco MJ signal transduction, inducing phenylpropanoid biosynthetic genes and the accumulation of phenylpropanoid-polyamine conjugates during stress.

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Section: Discussionmentioning

confidence: 94%

“…Interestingly, the two rate‐limiting ODC genes on the BY‐2 microarray showed differential induction during MJ treatments. One ODC gene, D89984 , which is identical to the NtODC‐2 gene (Xu et al. , 2004), was classified in group 1 together with several key phenylpropanoid genes.…”

Section: Discussionmentioning

confidence: 99%

A novel R2R3 MYB transcription factor NtMYBJS1 is a methyl jasmonate‐dependent regulator of phenylpropanoid‐conjugate biosynthesis in tobacco

Šimek²,

et al. 2006

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“…For example, it was shown that MeJA treatment led to significant increases in ODC activity in the leaves of barley seedlings . Similarly, MeJA differentially induced the expression of ODC gene family members in tobacco cell suspensions . Our previous work also showed that MeJA treatment stimulated the increase of ODC activity in cold‐stored tomato fruit .…”

Section: Introductionmentioning

confidence: 85%

Ornithine decarboxylase is involved in methyl jasmonate‐regulated postharvest quality retention in button mushrooms (Agaricus bisporus)

et al. 2018

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“…Five major classes of plant hormones are known to be involved in the regulation of nicotine biosynthesis, including JA, IAA, GA, ethylene, and ABA [32,[48][49][50][51]. It is known that JA treatment effectively induces nicotine biosynthesis in tobacco by regulating genes encoding enzymes of nicotine biosynthesis, including QPT, ODC, and PMT [34,38,52,53]. Yang et al [54] reported an analysis of the effects of JA on tobacco BY-2 cells, finding that transcript levels were increased for 12 ethylene response factors and 4 basic helix-loop-helix factors associated with alkaloid formation.…”

Section: Involvement Of Degs Responsive To Topping In Phytohormone Simentioning

confidence: 99%

Transcriptome analysis reveals key genes involved in the regulation of nicotine biosynthesis at early time points after topping in tobacco (Nicotiana tabacum L.)

Qin

Bai

et al. 2020

BMC Plant Biol

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Background: Nicotiana tabacum is an important economic crop. Topping, a common agricultural practice employed with flue-cured tobacco, is designed to increase leaf nicotine contents by increasing nicotine biosynthesis in roots. Many genes are found to be differentially expressed in response to topping, particularly genes involved in nicotine biosynthesis, but comprehensive analyses of early transcriptional responses induced by topping are not yet available. To develop a detailed understanding of the mechanisms regulating nicotine biosynthesis after topping, we have sequenced the transcriptomes of Nicotiana tabacum roots at seven time points following topping.Results: Differential expression analysis revealed that 4830 genes responded to topping across all time points. Amongst these, nine gene families involved in nicotine biosynthesis and two gene families involved in nicotine transport showed significant changes during the immediate 24 h period following topping. No obvious preference to the parental species was detected in the differentially expressed genes (DEGs). Significant changes in transcript levels of nine genes involved in nicotine biosynthesis and phytohormone signal transduction were validated by qRT-PCR assays. 549 genes encoding transcription factors (TFs), found to exhibit significant changes in gene expression after topping, formed 15 clusters based on similarities of their transcript level time-course profiles. 336 DEGs involved in phytohormone signal transduction, including genes functionally related to the phytohormones jasmonic acid, abscisic acid, auxin, ethylene, and gibberellin, were identified at the earliest time point after topping. Conclusions:Our research provides the first detailed analysis of the early transcriptional responses to topping in N. tabacum, and identifies excellent candidates for further detailed studies concerning the regulation of nicotine biosynthesis in tobacco roots.

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Differential induction of ornithine decarboxylase (ODC) gene family members in transgenic tobacco (Nicotiana tabacum L. cv. Bright Yellow 2) cell suspensions by methyl-jasmonate treatment

Cited by 37 publications

References 0 publications

A novel R2R3 MYB transcription factor NtMYBJS1 is a methyl jasmonate‐dependent regulator of phenylpropanoid‐conjugate biosynthesis in tobacco

A novel R2R3 MYB transcription factor NtMYBJS1 is a methyl jasmonate‐dependent regulator of phenylpropanoid‐conjugate biosynthesis in tobacco

Ornithine decarboxylase is involved in methyl jasmonate‐regulated postharvest quality retention in button mushrooms (Agaricus bisporus)

Transcriptome analysis reveals key genes involved in the regulation of nicotine biosynthesis at early time points after topping in tobacco (Nicotiana tabacum L.)

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