Differential gene expression between chronic hepatitis B and C hepatic lesion

Honda, Masao; Kaneko, Shuichi; Kawai, Hiroshi; Shirota, Yukihiro; Kobayashi, Kenichi

doi:10.1053/gast.2001.22468

Cited by 165 publications

(140 citation statements)

References 43 publications

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“…The protocol for preparing microarray slides containing 1080 cDNA clones has been described [5,6]. The genes to be spotted were selected from the Clontech atlas membrane array and obtained from IMAGE Consortium libraries through its distributor, Research Genetics (Huntsville, Ala., USA).…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA was isolated from frozen liver tissue samples using an RNA extraction kit [5], and first-strand cDNA was synthesised with Superscript II reverse transcriptase (GIBCO-BRL, Gaithersburg, Md., USA). Second-strand cDNA was synthesised with Escherichia coli DNA ligase and E. coli DNA polymerase (both from New England Biolabs, Beverly, Mass., USA) as described [5], and double-stranded cDNA was purified by phase lock gel (Eppendorf, Westbury, N.Y., USA) with phenol/chloroform extraction. We subsequently used double-stranded cDNA as a template for in vitro antisense RNA (aRNA) transcription and amplification using a MEGA script T7 kit (Ambion, Austin, Tex., USA) according to the manufacturer's protocol.…”

Section: Methodsmentioning

confidence: 99%

“…To label the probes, approximately 2 to 3 µg of aRNA was used as a template for first-strand cDNA synthesis with cyanine (Cy) 3-or Cy5-deoxyuridine triphosphate (Amersham Biosciences, Piscataway, N.J., USA) and Superscript II reverse transcriptase [5]. As a reference for each microarray analysis, we used aRNA samples prepared from the normal liver tissue of a 72-year-old man with a single liver tumour.…”

Section: Methodsmentioning

confidence: 99%

“…We have already used cDNA microarrays, consisting of 1080 human cDNAs, to characterise alterations that occur in various liver diseases [5,6]. More recently, microarray profiling of skeletal muscle tissue of Type 2 diabetic patients [7,8] and of healthy humans treated with insulin [9] has been reported.…”

Section: Introductionmentioning

confidence: 99%

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Genes for systemic vascular complications are differentially expressed in the livers of Type 2 diabetic patients

et al. 2004

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Aims/hypothesis. Type 2 diabetes is characterised by excessive hepatic glucose production and frequently leads to systemic vascular complications. We therefore analysed the relationship between the gene expression profile in the liver and the pathophysiology of Type 2 diabetes. Methods. Liver biopsy samples were obtained from twelve patients with Type 2 diabetes and from nine non-diabetic patients. To assay gene expression globally in the livers of both groups, we made complementary DNA (cDNA) microarrays consisting of 1080 human cDNAs. Relative expression ratios of individual genes were obtained by comparing cyanine 5-labelled cDNA from the patients with cyanine 3-labelled cDNA from reference RNA from the liver of a nondiabetic patient. Results. On assessing the similarities of differentially expressed genes, the gene expression profiles of the twelve diabetic patients formed a separate cluster from those of the non-diabetic patients. Of the 1080 genes assayed, 105 (9.7%) were up-regulated and 134 (12%) were down-regulated in the diabetic livers (p<0.005). The genes up-regulated in the diabetic patients included those encoding angiogenic factors such as vascular endothelial growth factor, endothelin and platelet-derived growth factor. They also included TGF superfamily genes such as TGFA and TGFB1 as well as bone morphogenetic proteins. Among the down-regulated genes in the diabetic patients were molecules defending against stress, e.g. flavin-containing monooxygenase and superoxide dismutase. Conclusions/interpretation. These findings suggest that livers of patients with Type 2 diabetes have gene expression profiles indicative of an increased risk of systemic vascular complications.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genes for systemic vascular complications are differentially expressed in the livers of Type 2 diabetic patients

et al. 2004

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“…Analysis of HCV-associated cirrhosis revealed an upregulation of proinflammatory, proapoptotic and pro-proliferative genes, which might reflect groups of genes being involved in HCV-related cirrhosis progressing to HCC (Honda et al, 2001;Shackel et al, 2002). Gene expression profiles of the HCV genotypes AFB 1 is enzymatically activated by human hepatocytes and the 8,9-AFB 1 oxide binds to the third base (G) in codon 249.…”

Section: Tp53 Mutations and Hcc Sp Hussain Et Almentioning

confidence: 99%