Differential expression of two genes located on the X chromosome between male and female in vitro-produced bovine embryos at the blastocyst stage

Gutiérrez-Adán, A.; Oter, M.; Martínez-Madrid, Belén; Pintado, Belén; Fuente, J. de la

doi:10.1002/(sici)1098-2795(200002)55:2<146::aid-mrd3>3.0.co;2-f

Cited by 116 publications

(81 citation statements)

References 34 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is the ratelimiting enzyme of the pentose phosphate pathway leading to the cellular production of NADPH (Rieger, 1992;Filosa et al, 2003) referred to as the guardian of the cell redox state. The levels of G6PDH RNA are higher in female morulae and blastocysts (Gutiérrez-Adan et al, 2000;Peippo et al, 2002). A higher level of this gene in female embryos should give them a better resistance to oxidative stress but this is not what we observed here.…”

Section: Discussioncontrasting

confidence: 85%

“…Several studies indicated that male embryos predominate among in vitro produced bovine blastocysts (Xu et al, 1992;Lonergan et al, 1999). In presence of glucose male embryos have a better ability to go through the morula-blastocyst transition (Larson et al, 2001), it is apparently also the case when serum is added to the culture medium (Gutiérrez-Adan et al, 2000). Under our culture conditions (5% FCS but no added glucose) day 8 blastocysts are predominantly male in the control group but this shift seemed reinforced by oxidative stress.…”

Section: Discussionmentioning

confidence: 57%

See 1 more Smart Citation

Impact of pro‐oxidant agents on the morula‐blastocyst transition in bovine embryos

Feugang¹,

Donnay²,

Mermillod³

et al. 2005

Molecular Reproduction Devel

View full text Add to dashboard Cite

Exposing day 5 bovine morulae to reactive oxygen species induces a delayed degeneration of some blastocysts on day 8 post-insemination (pi) but without affecting the blastocyst rates. The aim of this study was to characterize the resisting and the degenerating population of blastocysts. The kinetics of degeneration of the embryos exposed to the two prooxidant agents: 2,2 0 -azobis (2-amidinopropane) dihydrochloride (AAPH) and buthionine sulfoximine (BSO) was evaluated using time-lapse cinematography. With both agents the first signs of degeneration appeared at day 7.5 pi but the duration of the degeneration process was shorter in presence of AAPH than BSO (4.2 vs. 12.5 hr, ANOVA, P < 0.05). The resisting blastocysts derived from morulae with a larger diameter (mean diameter: 161 vs. 154 mm, ANOVA, P < 0.05) and showed an earlier cavitation (135 vs. 142 hpi, P < 0.05) than the degenerating ones. The profile of protein neosynthesis at day 7 was not affected by the treatment. The proportion of male embryos was more important in the resisting than in the degenerating population (70 vs. 55%, w 2 , P < 0.05) especially when the stress was induced by AAPH. The quality of the resisting embryos, measured by the total cell number and the rate of apoptosis, did not seem to be affected when compared to control embryos. In conclusion, resistance to oxidative stress seems related to the kinetics of development and/or the sex of the embryos. Resisting embryos apparently display a quality similar to untreated embryos. Mol. Reprod. Dev. 71: 339-346, 2005. ß 2005 Wiley-Liss, Inc.

show abstract

Section: Discussioncontrasting

confidence: 85%

Section: Discussionmentioning

confidence: 57%

Impact of pro‐oxidant agents on the morula‐blastocyst transition in bovine embryos

Feugang¹,

Donnay²,

Mermillod³

et al. 2005

Molecular Reproduction Devel

View full text Add to dashboard Cite

show abstract

“…The use of extended culture conditions during IVF and ICSI may favour selection of more male blastocysts for transfer as it is thought male embryos have greater pre-implantation developmental rates (8)(9)(10)(11)34). Evidence increasingly suggests that female and male embryos respond differently to in vitro culture conditions, due to the second X-chromosome present in females but not males retaining activity to the morula stage (35). The genes controlling glucose uptake and metabolism (glucose-6-phosphate dehydrogenase, G6PD) and anti-oxidants (hypoxanthine phosphoribosyl transferase, HPRT) are located on the X-chromosome, and thus female embryos have been reported to have higher glucose uptake and detoxification of oxygen radicals (36,37).…”

Section: Discussionmentioning

confidence: 99%

Effects of assisted reproductive technologies on human sex ratio at birth

Maalouf

Mincheva

Campbell

et al. 2014

Fertility and Sterility

View full text Add to dashboard Cite

“…Controversial results were found regarding G6PD expression, which was shown to be increased in some studies (Gutiérrez-Adán et al 2000, Peippo et al 2002, Wrenzycki et al 2002, Lopes et al 2007, Morton et al 2007, Bermejo-Alvarez et al 2011 and decreased in others (Merighe et al 2009), when comparing female and male IVP blastocysts. Considering that G6PD varies its expression according to respiration and metabolism (Lopes et al 2007), their different mRNA levels among systems might be related to stressful culture conditions.…”

Section: Discussionmentioning

confidence: 99%

HDAC inhibition decreases XIST expression on female IVP bovine blastocysts

Oliveira¹,

Saraiva

Cruz

et al. 2013

Reproduction

View full text Add to dashboard Cite

During initial development, both X chromosomes are active in females, and one of them must be silenced at the appropriate time in order to dosage compensate their gene expression levels to male counterparts. Silencing involves epigenetic mechanisms, including histone deacetylation. Major X chromosome inactivation (XCI) in bovine occurs between hatching and implantation, although in vitro culture conditions might disrupt the silencing process, increasing or decreasing X-linked gene expression. In this study, we aimed to address the roles of histone deacetylase inhibition by trichostatin A (TSA) on female preimplantation development. We tested the hypothesis that by enhancing histone acetylation, TSA would increase the percentage of embryos achieving 16-cell stage, reducing percentage of embryos blocked at 8-cell stage, and interfere with XCI in IVF embryos. We noticed that after TSA treatment, acetylation levels in individual blastomeres of 8-16 cell embryos were increased twofold on treated embryos, and the same was detected for blastocysts. Changes among blastomere levels within the same embryo were diminished on TSA group, as low-acetylated blastomeres were no longer detected. The percentage of embryos that reached the 5th cleavage cycle 118 h after IVF, analyzed by Hoechst staining, remained unaltered after TSA treatment. Then, we assessed XIST and G6PD expression in individual female bovine blastocysts by quantitative real-time PCR. Even though G6PD expression remained unaltered after TSA exposure, XIST expression was eightfold decreased, and we also detected a major decrease in the percentage of blastocysts expressing detectable XIST levels after TSA treatment. Based on these results, we conclude that HDAC is involved on XCI process in bovine embryos, and its inhibition might delay X chromosome silencing and attenuate aberrant XIST expression described for IVF embryos.

show abstract

Differential expression of two genes located on the X chromosome between male and female in vitro-produced bovine embryos at the blastocyst stage

Cited by 116 publications

References 34 publications

Impact of pro‐oxidant agents on the morula‐blastocyst transition in bovine embryos

Impact of pro‐oxidant agents on the morula‐blastocyst transition in bovine embryos

Effects of assisted reproductive technologies on human sex ratio at birth

HDAC inhibition decreases XIST expression on female IVP bovine blastocysts

Contact Info

Product

Resources

About