Differential expression of resistance to powdery mildew at the early stage of development in wheat line N0308

Abstract

ABSTRACT. Powdery mildew, caused by Blumeria graminis f. sp tritici (Bgt) is one of the devastating diseases of wheat and causes yield losses in temperate wheat growing regions. A wheat line, N0308 with resistance to powdery mildew was used in this study. A suppression subtractive hybridization cDNA library was constructed from the wheat leaves inoculated by Bgt at the two-leaf stage. The differentially expressed genes in response to Bgt infection in wheat were identified, and a total of 175 positive clones from the library were sequenced, and 90 expressed sequence tags (ESTs) were subjected to clustering, BLAST alignment, functional annotation, and classification into different categories. By comparing the EST sequences among the SSH-cDNA libraries, we analyzed gene expression patterns of 7 ESTs associated with the resistance reaction of powdery mildew by using semi-quantitative reverse transcription-polymerase chain reaction. The expression of 5 genes (sulfatase, pathogenesis-related protein 17, betacarbonic anhydrase 2, thioredoxin h-like protein, and coronatineinsensitive) transcripts was induced, and the transcript levels of these genes were the highest at 72 h after Bgt infection, while those of 2 genes (violaxanthin de-epoxidase and gag-pol-polyprotein) were the highest level at 12 and 18 h post-infection, respectively. These findings suggest that these genes are induced at an early stage of infection and are transcriptionally activated for the host defense response.

Selection of Genes Associated with Variations in the Circle of Willis in Gerbils Using Suppression Subtractive Hybridization

“…From 12 SSH libraries, we identified 304 gene sequences in which 23% were ESTs. The percentage was less than that in previous reports [ 21 , 22 ], which might be attributed that in this study we used inbred gerbils with high genetic consistency. Most of the identified genes were associated with proliferation, differentiation, migration, and apoptosis, all of which may influence vascular development.…”

Mechanisms of powdery mildew resistance of wheat – a review of molecular breeding

“…Security of wheat production is of great economic importance globally as it is a key staple food crop. Wheat cultivation can be found in many areas of the world, particularly in regions with maritime and semicontinental climates, and this diversity presents multiple constraints on productivity (Alam et al , 2014). Despite the growing scarcity of arable land and shifting of agriculture to more marginal lands, the production volume of wheat is projected to increase (Peng et al , 2011; Placido et al , 2013).…”

Genomic identification, phylogeny, and expression analysis of MLO genes involved in susceptibility to powdery mildew in Fragaria vesca

“…Recently, PM disease resistance has been reported in cucumber, wheat, and grapevine (Zhou et al, 2013;Alam et al, 2014;Lu et al, 2014;Zhang et al, 2014). In strawberry (Fragaria x ananassa Duchesne), cleaved amplified polymorphic sequence markers related to PM resistance have been developed (Heejeong et al, 2015), and interactions between strawberry and PM have been revealed using the RNA-seq method (Sun et al, 2015).…”