Differential expression of pro-inflammatory cytokines in endometrial tissue of buffaloes with clinical and sub-clinical endometritis

Loyi, Tumnyak; Kumar, Harendra; Nandi, Sukdeb; Mathapati, Basavaraj; Patra, Manas Kumar; Pattnaik, Bramhadev

doi:10.1016/j.rvsc.2012.09.008

Cited by 37 publications

(30 citation statements)

References 22 publications

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“…Various threshold ranging from 5-18% of PMNs in endometrial cytology have been reported in cattle, however, most of the studies in cattle and buffalo used >5% PMN cut-off to diagnose cytological endometritis (Gilbert et al, 2005;Loyi et al, 2013). Histopathological findings of buffalo uterus with cytological endometritis as observed in the present study are almost similar to observations in biopsy sample of repeat breeding cows with subclinical endometritis.…”

Section: Discussionsupporting

confidence: 83%

“…Endometrial cytology smear of the apparently normal uterus with >5% PMN cells ( Fig. 1) and no gross inflammatory lesions were classified as Cytological Endometritis (CE) (Gilbert et al, 2005;Loyi et al, 2013). Uterus filled with purulent or mucopurulent material and presence of inflammatory lesions like haemorrhage, necrotic lesions and exudate debris in the carancular and intercarancular areas in the endometrium was categorized as Purulent Endometritis (PE) (Fig.…”

Section: Gross Examination and Classification Of Different Affectionsmentioning

confidence: 99%

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Histopathological Evaluation of Important Uterine Pathological Affections in Riverine Buffalo (Bubalus bubalis): An Abattoir Study

Chethan¹,

Singh²,

Karikalan³

et al. 2015

Asian J. of Animal and Veterinary Advances

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Infertility or sterility due to uterine pathology is one of the major reasons for culling of buffalo in India. Abattoirs serve as a good source of biological samples in order to study different pathological conditions. Major affections of uterus in buffalo include endometritis, metritis, pyometra, hydrometra, mucometra and certain congenital abnormalities. Mostly diagnosis of such cases is based on gross morphological examination observed in abattoir or sometime on clinical and post-mortem examination. Histological based diagnosis of major affections of uterus in buffalo is less and as such very few reports are available especially on hydrometra and mucometra conditions. The present study reports the morphological and histopathological evaluation of cytological and purulent endometritis, hydrometra and mucometra conditions in riverine buffalo.

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Section: Discussionsupporting

confidence: 83%

Section: Gross Examination and Classification Of Different Affectionsmentioning

confidence: 99%

Histopathological Evaluation of Important Uterine Pathological Affections in Riverine Buffalo (Bubalus bubalis): An Abattoir Study

Chethan¹,

Singh²,

Karikalan³

et al. 2015

Asian J. of Animal and Veterinary Advances

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“…However, less is known regarding the role of CD14 in buffalo. There was one recent research report about the role of CD14 in the endometrial tissue of buffaloes [12]. In this study, we determined that treatment of buffalo mononuclear macrophages with LPS had a significant effect on the expression of endogenous CD14 and TLR4 signaling pathway-related genes.…”

Section: Discussionmentioning

confidence: 81%

“…These results are consistent with those from previous studies of practical buffalo inflammatory disease. Loyi observed the differential expression of several proinflammatory cytokines in the endometrium of buffalo with clinical and subclinical endometritis [12,32], and a significant upregulation of CD14 (1-to 2-fold), IL-6 (15-to 36-fold) and TNF-α (10-to 11-fold) mRNA in SCE. Our results indicate that the expression pattern of CD14 and other related cytokine genes in cultured buffalo mononuclear macrophages in response to LPS stimulation is similar to that reported for in vivo expression.…”

Section: Discussionmentioning

confidence: 99%

“…The gene function of CD14 and several inflammatory cytokines has been widely explored in certain diseases that are caused by Gram-negative bacteria in humans, mice and livestock. Examples include endometritis, Brucella and mastitis [12][13][14]. Most of our understanding of the molecular regulatory mechanism of CD14 has been based on the results of mouse RAW264.7 macrophage studies [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

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The effect of buffalo CD14 shRNA on the gene expression of TLR4 signal pathway in buffalo monocyte/macrophages

Huang

et al. 2014

Cellular and Molecular Biology Letters

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CD14 plays a crucial role in the inflammatory response to lipopolysaccharide (LPS), which interacts with TLR4 and MD-2 to enable cell activation, resulting in inflammation. Upstream inhibition of the inflammation pathway mediated by bacterial LPS, toll-like receptor 4 (TLR4) and cluster of differentiation antigen 14 (CD14) was proven to be an effective therapeutic approach for attenuating harmful immune activation. To explore the effect of CD14 downregulation on the expression of TLR4 signaling pathway-related genes after LPS stimulation in buffalo (Bubalus bubalis) monocyte/macrophages, effective CD14 shRNA sequences were screened using qRT-PCR and FACS analysis with buffalo CD14 shRNA lentiviral recombinant plasmids (pSicoR-GFP-shRNA) and buffalo CD14 fusion expression plasmids (pDsRed-N1-buffalo CD14) co-transfected into HEK293T cells via liposomes. Of the tested shRNAs, shRNA-1041 revealed the highest knockdown efficiency (p < 0.01). When buffalo peripheral blood monocyte/macrophages were infected with shRNA-1041 lentivirus and stimulated with LPS, the expression of endogenous CD14 was significantly decreased by CD14 shRNA (p < 0.01), and the mRNA expression levels of TLR4, IL-6 and TNF-α were also significantly downregulated compared to the control groups (p < 0.01). These results demonstrated that the knockdown of endogenous CD14 had clear regulatory effects on the signal transduction of TLR4 after stimulation with LPS. These results may provide a better understanding of the molecular mechanisms of CD14 regulation in the development of several buffalo diseases.

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Pro-inflammatory cytokine expression studies of subclinical and clinical endometritis in endometrial tissues of buffaloes

Bhadaniya

Prasad

Savsani

et al. 2019

Trop Anim Health Prod

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Differential expression of pro-inflammatory cytokines in endometrial tissue of buffaloes with clinical and sub-clinical endometritis

Cited by 37 publications

References 22 publications

Histopathological Evaluation of Important Uterine Pathological Affections in Riverine Buffalo (Bubalus bubalis): An Abattoir Study

Histopathological Evaluation of Important Uterine Pathological Affections in Riverine Buffalo (Bubalus bubalis): An Abattoir Study

The effect of buffalo CD14 shRNA on the gene expression of TLR4 signal pathway in buffalo monocyte/macrophages

Pro-inflammatory cytokine expression studies of subclinical and clinical endometritis in endometrial tissues of buffaloes

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