Differential expression of ompA, ompB, pyk, nlpD and Cpn0585 genes between normal and interferon-γ treated cultures of Chlamydia pneumoniae

Mathews, Sarah A.; George, Carmel; Flegg, Cameron; Stenzel, Deborah; Timms, Peter

doi:10.1006/mpat.2000.0435

Cited by 59 publications

(67 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cpn0585 became detectable 24 hours (Fig. 3A, panel d) while IncA as early as 12 hours after C. pneumoniae infection (k & k1), suggesting that Cpn0585 is a late protein, which is consistent with a previous observation that Cpn0585 transcript was only detected at a late stage of C. pneumoniae infection [15]. Both Cpn0585 and IncA proteins were likely secreted to the inclusion membrane once they became detectable since these Inc proteins always surrounded C. pneumoniae organisms (white arrows in panels d & k1 of Fig.3A).…”

Section: Time Course Expression Of Cpn0585 and Cca00156 During Chlamysupporting

confidence: 90%

Localization of the hypothetical protein Cpn0585 in the inclusion membrane of Chlamydia pneumoniae-infected cells

Luo¹,

Jia²,

Zhong³

et al. 2007

Microbial Pathogenesis

View full text Add to dashboard Cite

Cpn0585, encoded by a hypothetical open reading frame in Chlamydia pneumoniae genome, was detected in the inclusion membrane during C. pneumoniae infection using both polyclonal and monoclonal antibodies raised with Cpn0585 fusion protein. The anti-Cpn0585 antibodies specifically recognized the endogenous Cpn0585 without cross-reacting with IncA (a known inclusion membrane protein of C. pneumoniae) or other control antigens. A homologue of Cpn0585 in the C. caviae species (encoded by the ORF CCA00156) was also localized in the inclusion membrane of the C. caviae-infected cells. The Cpn0585 protein became detectable 24 hours while CCA00156 as early as 8 hours after infection. Once expressed, both proteins remained in the inclusion membrane throughout the rest of infection course.

show abstract

Section: Time Course Expression Of Cpn0585 and Cca00156 During Chlamysupporting

confidence: 90%

Localization of the hypothetical protein Cpn0585 in the inclusion membrane of Chlamydia pneumoniae-infected cells

Luo¹,

Jia²,

Zhong³

et al. 2007

Microbial Pathogenesis

View full text Add to dashboard Cite

show abstract

“…ϩ T cells between CpnCTL7-vaccinated and Cpnreinfected mice were for peptides 37 and 39, and these epitopes are respectively from the OmpB and Cpn0585 Ags, whose genes are up-regulated during Cpn persistence (69). Finally, the lungs of minigene-immunized Cpn-challenged mice showed minimal inflammatory pathology, unlike the chronic inflammation associated with persistent chlamydial infections (5).…”

Section: Discussionmentioning

confidence: 99%

A CD8+ T Cell Heptaepitope Minigene Vaccine Induces Protective Immunity againstChlamydia pneumoniae

Pinchuk

Starcher

Livingston³

et al. 2005

The Journal of Immunology

View full text Add to dashboard Cite

An intact T cell compartment and IFN-γ signaling are required for protective immunity against Chlamydia. In the mouse model of Chlamydia pneumoniae (Cpn) infection, this immunity is critically dependent on CD8+ T cells. Recently we reported that Cpn-infected mice generate an MHC class I-restricted CD8+ Tc1 response against various Cpn Ags, and that CD8+ CTL to multiple epitopes inhibit Cpn growth in vitro. Here, we engineered a DNA minigene encoding seven H-2b-restricted Cpn CTL epitopes, the universal pan-DR epitope Th epitope, and an endoplasmic reticulum-translocating signal sequence. Immunization of C57BL/6 mice with this construct primed IFN-γ-producing CD8+ CTL against all seven CTL epitopes. CD8+ T cell lines generated to minigene-encoded CTL epitopes secreted IFN-γ and TNF-α and exhibited CTL activity upon recognition of Cpn-infected macrophages. Following intranasal challenge with Cpn, a 3.6 log reduction in mean lung bacterial numbers compared with control animals was obtained. Using a 20-fold increase in the Cpn challenging dose, minigene-vaccinated mice had a 60-fold reduction in lung bacterial loads, compared with controls. Immunization and challenge studies with β2-microglobulin−/− mice indicated that the reduction of lung Cpn burdens was mediated by the MHC class I-dependent CD8+ T cells to minigene-included Cpn CTL epitopes, rather than by pan-DR epitope-specific CD4+ T cells. This constitutes the first demonstration of significant protection achieved by immunization with a CD8+ T cell epitope-based DNA construct in a bacterial system and provides the basis for the optimal design of multicomponent anti-Cpn vaccines for humans.

show abstract

“…The development of persistence in vitro is characterized by a reduction of the intracellular growth rate of C. pneumoniae and by the appearance of morphologically aberrant reticulate bodies (27). Persistent C. pneumoniae has been shown to be metabolically active (1,10,32), but the period of persistence as well as the influence on the antibody titer of the host is unknown. There is emerging evidence that persistent C. pneumoniae might be implicated in the pathogenesis of several chronic and sometimes destructive diseases of the lung and the nervous system and atherosclerosis (13, 29, 41-43, 48, 52).…”

mentioning

confidence: 99%

Comparison of Eleven Commercial Tests for Chlamydia pneumoniae -Specific Immunoglobulin G in Asymptomatic Healthy Individuals

et al. 2002

View full text Add to dashboard Cite

The seroprevalence of anti-Chlamydia pneumoniae-specific immunoglobulin G (IgG) antibodies is high in the adult population. Experience is required to perform a microimmunofluorescence test (MIF), the current "gold standard" for serological diagnosis, and the assay still lacks standardization. Partially automated enzymelinked immunosorbent assays (ELISAs) and enzyme immunoassays (EIAs), which are more standardized and for which the reading of results is less subjective, have been developed. The different commercially available serological tests differ in their sensitivities and specificities, depending primarily on the antigen used. Therefore, we evaluated 11 different tests (10 were species specific, 1 was genus specific) for IgG antibodies using serum samples of 80 apparently healthy volunteers. The interpretation of the results was based on the results of the gold standard, MIF: a sample was judged positive if it was positive by at least three of the four different MIFs. Based on this internal standard, we found that 71% of the samples were positive, while 8% were false positive by some tests. The correlations between the results of the different MIFs ranged from 83 to 99%, and the correlations between the results of the MIFs and the different ELISAs and EIAs ranged from 78 to 98%. Comparison of the IgG titers measured by MIF showed good agreement (r ‫؍‬ 0.76 to 0.91). This analysis revealed that some ELISAs and EIAs fail to detect low IgG titers. The specificities of the species-specific tests varied from 95 to 100%, and the sensitivities varied from 58 to 100%. These results indicate that serological assays for the detection of anti-C. pneumoniae-specific IgG vary greatly in their sensitivities and specificities. MIF must still be considered the best method for the detection of IgG in apparently healthy subjects, but the sensitivities and specificities of new ELISAs approximate those of MIFs.

show abstract

Differential expression of ompA, ompB, pyk, nlpD and Cpn0585 genes between normal and interferon-γ treated cultures of Chlamydia pneumoniae

Cited by 59 publications

References 16 publications

Localization of the hypothetical protein Cpn0585 in the inclusion membrane of Chlamydia pneumoniae-infected cells

Localization of the hypothetical protein Cpn0585 in the inclusion membrane of Chlamydia pneumoniae-infected cells

A CD8+ T Cell Heptaepitope Minigene Vaccine Induces Protective Immunity againstChlamydia pneumoniae

Comparison of Eleven Commercial Tests for Chlamydia pneumoniae -Specific Immunoglobulin G in Asymptomatic Healthy Individuals

Contact Info

Product

Resources

About