2016
DOI: 10.1155/2016/6738701
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Abstract: NADPH oxidases (NOX) are important sources of reactive oxygen species (ROS) in skeletal muscle, being involved in excitation-contraction coupling. Thus, we aimed to investigate if NOX activity and expression in skeletal muscle are fiber type specific and the possible contribution of this difference to cellular oxidative stress. Oxygen consumption rate, NOX activity and mRNA levels, and the activity of catalase (CAT), glutathione peroxidase (GPX), and superoxide dismutase (SOD), as well as the reactive protein … Show more

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Cited by 35 publications
(31 citation statements)
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“…Studies report that in oxidative part of the gastrocnemius muscle, the SOD enzyme was increased in the hyperglycemic state, as well as with the type 1 high-intensity physical training. In the case of SOD (with lower mitochondrial number), similar results have been reported previously for training at 60% of the maximum velocity in animals without the pathology [33,46,47]. It was also found that NOX was higher in the soleus and RG muscles, compared to the WG muscle, which could have been due to lower vascular development in the WG muscle [47].…”
Section: Discussionsupporting
confidence: 87%
“…Studies report that in oxidative part of the gastrocnemius muscle, the SOD enzyme was increased in the hyperglycemic state, as well as with the type 1 high-intensity physical training. In the case of SOD (with lower mitochondrial number), similar results have been reported previously for training at 60% of the maximum velocity in animals without the pathology [33,46,47]. It was also found that NOX was higher in the soleus and RG muscles, compared to the WG muscle, which could have been due to lower vascular development in the WG muscle [47].…”
Section: Discussionsupporting
confidence: 87%
“…In adult rat whole muscle lysates, NOX2, NOX4, and DUOX1 mRNA are expressed, whereas data on the expression of DUOX2 are conflicting (220,339). NOX2 and NOX4 exhibit a fiber typedependent mRNA expression, higher in slow-twitch oxidative compared with fast-twitch glycolytic muscles (220). At the protein level, the expression of all NOX2 subunits and NOX4 has been confirmed by immunoblotting in isolated mouse single muscle fibers (309).…”
Section: B Expression Of Nox Isoforms In Skeletal Musclementioning
confidence: 99%
“…In immortalized mouse skeletal muscle C2C12 cell culture, NOX1, 2, 4, DUOX1, and DUOX2 are detectable at the messenger RNA (mRNA) level (105). In adult rat whole muscle lysates, NOX2, NOX4, and DUOX1 mRNA are expressed, whereas data on the expression of DUOX2 are conflicting (220,339). NOX2 and NOX4 exhibit a fiber typedependent mRNA expression, higher in slow-twitch oxidative compared with fast-twitch glycolytic muscles (220).…”
Section: B Expression Of Nox Isoforms In Skeletal Musclementioning
confidence: 99%
“…The localization of H 2 O 2 is also supported by the low permeability of H 2 O 2 to plasma membranes (P m = 2 • 10 − 4 -10 − 3 cm • s − 1 [51]). As a final note, considering that NADPH oxidases are likely the most important sources of H 2 O 2 in skeletal muscle [44], it is good to keep in mind that NADPH oxidase activity and expression is higher in type I than in type II muscles [52]. Somewhat to the contrary, evidence suggests that the H 2 O 2 emission from mitochondria in permeabilized rat muscle fiber was two-to three-fold greater in type II fibers [3].…”
Section: Oxidantsmentioning
confidence: 99%