Differential expression of miRNAs and related mRNAs during heat stress in buffalo heifers

Yadav, Poonam; Yadav, B. R.; Swain, Dilip Kumar; Anand, Mukul; Yadav, Sarita; Madan, Arun Kumar

doi:10.1016/j.jtherbio.2021.102904

Cited by 6 publications

(9 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…increase. An increase in SOD activity in response to heat stress was also reported in buffaloes (Kumar et al, 2011;Yadav et al, 2021a). The results of present study revealed that intrinsic antioxidative defense system could minimize the increased ROS levels at a THI of 85.…”

Section: Resultssupporting

confidence: 80%

Heat stress responses to increasing temperature humidity index (THI) in lactating Murrah buffalo

Yadav¹,

Yadav²,

Madan³

et al. 2022

Buffalo Bull.

View full text Add to dashboard Cite

The present study was conducted to determine the effect of increasing temperature humidity index (THI) on lactating Murrah buffalo. The study was conducted on six lactating buffaloes which were offered the same basal diet and were blocked by days in milk, milk yield and parity. The study was conducted during May, June and July with average THI of 79.88, 80.57 and 85.36, respectively. Milk yield was recorded twice a day at 06:00 and 18:00 h and physiological parameters were recorded and blood was sampled on 30th day of each month at 14:30 h. The results showed a significant decrease (P<0.05) in milk yield in buffaloes only at a THI of 85.36 as compared to THI up to 80.57. The rectal temperature (RT) did not change (P=0.580) with increasing THI up to 85.36, however, the respiration rate (RR) and pulse rate (PR) changed (P<0.05) progressively with increasing THI. Leucocytopnoea, lymphocytopnoea and neutrophilia; and alteration in serum urea, alkaline phosphatase activity, reactive oxygen species and phosphorus concentration were observed only at a THI of 85.36 as compared to THI up to 80.57. Similarly, significant (P<0.05) alteration in serum T4, cortisol and prolactin levels were observed at a THI of 85.36 as compared to THI up to 80.57. It can be concluded that lactating buffaloes experienced mild heat stress up to a THI of 80.57 (May and June) and a moderate type of heat stress at a THI 85.36 (July).

show abstract

Section: Resultssupporting

confidence: 80%

Heat stress responses to increasing temperature humidity index (THI) in lactating Murrah buffalo

Yadav¹,

Yadav²,

Madan³

et al. 2022

Buffalo Bull.

View full text Add to dashboard Cite

show abstract

“…Results also suggest that under a given heat stress exposure (THI; 87-90), adaptive responses in the rumen ecosystem could not produce detectable harmful effects in the fermentation pattern. In our previous study [36], heat stress exposure exhibited a moderate level of acclimatiza-tion responses in terms of hematological, biochemical, oxidative stress, endocrine, and heat shock protein markers which corroborates with the moderate effect of heat stress on the rumen microbial ecosystem and its functional attributes.…”

Section: Discussionsupporting

confidence: 80%

“…However, physiobiochemical changes in the host directly affect rumen microbial diversity, fermentation, and nutrient utilization [15] besides heat stress mediated changes in the rumen itself [19]. In our previous study, we have demonstrated the effect of heat stress on physiobiochemical, redox, endocrine parameters, and miRNAs mediated heat shock protein mRNA regulation [36], and in continuation, the present experiment is aimed at investigating the effect of heat stress on rumen microbiome and fermentation pattern in buffalo heifers.…”

Section: Discussionmentioning

confidence: 81%

“…The average maximum and minimum ambient temperature and relative humidity during thermoneutral exposure were 29.8 °C and 20.3 °C, and 54.8%, respectively, whereas during heat stress exposure, the values were 41.6 °C and 28.8 °C, and 36.4%, respectively. The combination of temperature and humidity for thermoneutral and heat stress conditions in the climatic chambers was used as standardized in the case of buffalo heifers in our previous study [36]. These combinations were adjusted in such a way that there was a minimum difference between the environmental and climate chamber THI during the entry and exit of the animals from the climate chamber.…”

Section: Experimental Designmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Heat Stress on Rumen Microbial Diversity and Fermentation Pattern in Buffalo

Yadav

Kumar

et al. 2022

Advanced Gut & Microbiome Research

View full text Add to dashboard Cite

In this study, changes in rumen microbiome and fermentation were examined during heat stress (HS) in buffaloes. Six buffalo heifers were exposed to thermoneutral ( THI = 72 ) and HS ( THI = 87 – 90 ) conditions for 6 h between 10 AM and 4 PM for 21 days in the climatic chamber. A digestibility trial with four-day collection period was conducted at the end of the study, and rumen liquor was collected on day 21. At the phylum level, an increased ( p < 0.05 ) abundance of Firmicutes and a decreased ( p < 0.05 ) abundance of Proteobacteria and Planctomycetes were observed during HS compared to thermoneutral conditions. The abundance of 6 different genera of phylum Firmicutes, YRC22 (phylum Bacteroidetes), and Stenotrophomonas (phylum Proteobacteria) was increased ( p < 0.05 ), whereas B. firmus, Lysinibacillus, Bacteroides unclassified genus (family Enterobacteriaceae), and Methanobrevibacter decreased ( p < 0.05 ) during HS compared to control. Eight new genera from four different phyla were detected after HS exposure, whereas one genus was not detected in the rumen after HS exposure, which was present during thermoneutral conditions. Dry matter intake, volatile fatty acid concentration, and digestibility did not change ( p > 0.05 ) during HS. It can be concluded that the resilience of the rumen microbial population invokes adaptive responses by changing their abundance to minimize the adverse effects on fermentation and digestibility in buffalo heifers.

show abstract

“…HSP genes are induced by cellular stress to protect cells against apoptosis and provide thermotolerance [42][43][44]. HSPA1A and HSPA6 are the most heat-shockinducible HSP members [45].…”

Section: Discussionmentioning

confidence: 99%

The alteration of N6-methyladenosine (m6A) modification at the transcriptome-wide level in response of heat stress in bovine mammary epithelial cells

Zhang

Wang

et al. 2022

BMC Genomics

View full text Add to dashboard Cite

Background Heat stress has a substantial negative economic impact on the dairy industry. N6-methyladenosine (m6A) is the most common internal RNA modification in eukaryotes and plays a key role in regulating heat stress response in animals. In dairy cows, however, this modification remains largely unexplored. Therefore, we examined the effects of heat stress on the m6A modification and gene expression in bovine mammary epithelial cells to elucidate the mechanism of heat stress response. In this study, Mammary alveolar cells-large T antigen (MAC-T) cells were incubated at 37 °C (non-heat stress group, NH) and 40 °C (heat stress group, H) for 2 hours, respectively. HSP70, HSF1, BAX and CASP3 were up regulated in H group compared with those in the NH group. Results Methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) were conducted to identify m6A peaks and to produce gene expression data of MAC-T cells in the H and NH groups. In total, we identified 17,927 m6A peaks within 9355 genes in the H group, and 18,974 peaks within 9660 genes in the NH groups using MeRIP-seq. Compared with the NH group, 3005 significantly differentially enriched m6A peaks were identified, among which 1131 were up-regulated and 1874 were down-regulated. In addition, 1502 significantly differentially expressed genes were identified using RNA-seq, among which 796 were up-regulated and 706 were down-regulated in the H group compared to the NH group. Furthermore, 199 differentially expressed and synchronously differentially methylated genes were identified by conjoint analysis of the MeRIP-seq and RNA-seq data, which were subsequently divided into four groups: 47 hyper-up, 53 hyper-down, 59 hypo-up and 40 hypo-down genes. In addition, GO enrichment and KEGG analyses were used to analyzed the potential functions of the genes in each section. Conclusion The comparisons of m6A modification patterns and conjoint analyses of m6A modification and gene expression profiles suggest that m6A modification plays a critical role in the heat stress response by regulating gene expression.

show abstract

Differential expression of miRNAs and related mRNAs during heat stress in buffalo heifers

Cited by 6 publications

References 60 publications

Heat stress responses to increasing temperature humidity index (THI) in lactating Murrah buffalo

Heat stress responses to increasing temperature humidity index (THI) in lactating Murrah buffalo

Effect of Heat Stress on Rumen Microbial Diversity and Fermentation Pattern in Buffalo

The alteration of N6-methyladenosine (m6A) modification at the transcriptome-wide level in response of heat stress in bovine mammary epithelial cells

Contact Info

Product

Resources

About