Differential expression of long non‑coding RNA and mRNA in children with Henoch‑Sch�nlein purpura nephritis

Abstract

Long non-coding RNAs (lncRNAs) serve an essential role in regulating immunological functions. However, their impact on Henoch-Schönlein purpura nephritis (HSPN), has remained elusive. The present study determined the expression of lncRNAs and mRNAs in the peripheral blood of 6 children with HSPN and recruited 4 healthy children for comparative study. High-throughput sequencing revealed outstanding differences in lncRNA and mRNA expression, which were verified through reverse transcription-quantitative polymerase chain reaction. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were used to investigate the associated biological functions and possible mechanisms of lncRNAs and mRNAs in HSPN. A total of 820 differentially expressed lncRNAs between the two groups were identified, of which 34 were upregulated and 786 were downregulated. Simultaneously, a total of 3,557 mRNAs were also identified to be differentially expressed, of which 1,232 were upregulated and 2,325 were downregulated. The results revealed that the expression of lncRNAs including ENST00000378432, ENST00000571370, uc001kfc.1 and uc010qna.2 was decreased in HSPN patients compared with that in healthy controls. These lncRNAs were associated with the p53 signaling pathway and apoptosis-associated genes (AKT2, tumor protein 53, phosphatase and tensin homolog and FAS). Further studies of those lncRNAs will be performed to elucidate their functions in apoptosis. Complete raw data files were deposited in the Gene Expression Omnibus (GEO) at National Center for Biotechnology information under the GEO accession no. GSE102114 ().

An Integrated Transcriptomic and Proteomic Analysis Identifies Significant Novel Pathways for Henoch-Schönlein Purpura Nephritis Progression

“…In particular, these mRNAs and proteins were ranked according to their P values (in ascending order), and the functions of the top 10 mRNAs and proteins were searched in GeneCards and UniProt databases. They mainly were found to be involved in immunity, apoptosis, platelet and coagulation, and tumor necrosis, consistent with the pathway enrichment results, and those functions were related to nephritis and renal injury [ 10 , 15 , 19 ]. It is worth noting that most proteins were differentially expressed between different types of HSPN while their corresponding mRNAs were not differentially expressed between those groups (Supplementary Table S 2 and Supplementary Table S 3 ).…”

“…Of these, RPS17-201 is of most promise with it being found to be continuously downregulated throughout HSPN disease. RPS17 encodes a ribosomal protein, involved in the generation of serum IgA [ 15 ]. Furthermore, it was found that most DEGs were only differentially expressed in either early HSPN or late HSPN (Supplementary Table S 2 and Supplementary Table S 3 , respectively).…”

“…Integration of different “omics” techniques facilitates the investigation of the possible mechanism from a systems biology point of view, providing a deeper understanding than any single “omics” study could do alone [ 14 ]. Previously, a study from China reported differential expression of long noncoding RNAs and mRNAs between children with HSPN and healthy children [ 15 ]; however, this study lacked proteomic data. Protein aggregation is known to be fundamental to HSPN pathogenesis, and the roles of abnormal proteins in HSPN are widely discussed [ 1 – 3 ].…”

“…BioMed Research International enrichment results, and those functions were related to nephritis and renal injury [10,15,19]. It is worth noting that most proteins were differentially expressed between different types of HSPN while their corresponding mRNAs were not differentially expressed between those groups (Supplementary Table S2 and Supplementary Table S3).…”

Whole Transcriptome Analysis of Obese Adipose Tissue Suggests u001kfc.1 as a Potential Regulator to Glucose Homeostasis

“…To date, uc001kfc.1 (n381526, NONHSAT015462.2) has been only reported in two literatures about cholesteatoma (Gao et al, 2018) and purpura nephritis (Pang et al, 2019). According to NONCODE, this lncRNA is highly expressed in human adipose tissue and no homologues in other non-human animal models has been reported to date (Fang et al, 2018).…”