Differential expression of carotenoid biosynthetic pathway genes in two contrasting tomato genotypes for lycopene content

Pandurangaiah, Shilpa; Ravishankar, K. V.; Shivashankar, K.; Sadashiva, A. T.; Pillakenchappa, Kavitha; Narayanan, Sunil Kumar

doi:10.1007/s12038-016-9602-4

Cited by 17 publications

(12 citation statements)

References 21 publications

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“…The red-colored tomato fruit mainly contains lycopene (red carotenoid), which accounts for 90% of the total carotenoid content, and β-carotene (yellow carotenoid). Lycopene is catalyzed by lycopene β-cyclase (CYC-B) to form β-carotene, and the ratio of these two carotenoids affects the fruit color [ 23 ]. As expected, the carotenoid content of the psy1 single mutant was significantly lower than that of other genotypes because of the abnormal accumulation of lycopene ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Recoloring tomato fruit by CRISPR/Cas9-mediated multiplex gene editing

Yang

Ali

Lin

et al. 2022

Horticulture Research

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Fruit color is an important horticultural trait, which greatly affects consumer preferences. In tomato, fruit color is determined by the accumulation of different pigments, such as carotenoids in the pericarp and flavonoids in the peel, along with the degradation of chlorophyll during fruit ripening. Since fruit color is a multigenic trait, it takes years to introgress all color-related genes in a single genetic background via traditional cross-breeding, and the avoidance of linkage drag during this process is difficult. Here, we proposed a rapid breeding strategy to generate tomato lines with different colored fruits from red-fruited materials by CRISPR/Cas9-mediated multiplex gene editing of three fruit color-related genes (PSY1, MYB12, and SGR1). Using this strategy, the red-fruited cultivar Alisa Craig has been engineered to a series of tomato genotypes with different fruit colors, including yellow, brown, pink, light-yellow, pink-brown, yellow-green, and light green. Compared to traditional cross-breeding, this strategy requires less time and can obtain transgene-free plants with different colored fruits in less than one year. Most importantly, it does not alter other important agronomic traits like yield and fruit quality. Our strategy has great practical potential for tomato breeding and serves as a reference for improving multigene-controlled traits of horticultural crops.

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Section: Resultsmentioning

confidence: 99%

Recoloring tomato fruit by CRISPR/Cas9-mediated multiplex gene editing

Yang

Ali

Lin

et al. 2022

Horticulture Research

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“…Our previous work reported that the transcript level of CYC-B regulates orange color in tomato fruit [16]. Researchers focused on carotenoid content [17, 35] were also reported that regulation of CYC-B increases the accumulation of β-carotene in the tomato fruit during ripening. Similar results were observed during fruit development in bell pepper, a related species [36–39].…”

Section: Resultsmentioning

confidence: 99%

Molecular breeding of a novel orange-brown tomato fruit with enhanced beta-carotene and chlorophyll accumulation

et al. 2017

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BackgroundTomatoes provide a significant dietary source of the carotenoids, lycopene and β-carotene. During ripening, carotenoid accumulation determines the fruit colors while chlorophyll degradation. These traits have been, and continue to be, a significant focus for plant breeding efforts. Previous work has found strong evidence for a relationship between CYC-B gene expression and the orange color of fleshy fruit. Other work has identified a point mutation in SGR that impedes chlorophyll degradation and causes brown flesh color to be retained in some tomato varieties.MethodsWe crossed two inbred lines, KNY2 (orange) and KNB1 (brown) and evaluated the relationship between these genes for their effect on fruit color. Phenotypes of F2 generation plants were analyzed and a novel ‘orange-brown’ fruit color was identified.ResultsWe confirm two SNPs, one in CYC-B and another in SGR gene sequence, associated with segregation of ‘orange-brown’ fruit color in F2 generation. The carotenoid and chlorophyll content of a fleshy fruit was assessed across the different phenotypes and showed a strong correlation with expression pattern of carotenoid biosynthesis genes and SGR function. The orange-brown fruit has high β-carotene and chlorophyll. Our results provide valuable information for breeders to develop tomato fruit of a novel color using molecular markers.Electronic supplementary materialThe online version of this article (doi:10.1186/s41065-016-0023-z) contains supplementary material, which is available to authorized users.

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“…This study will help to improve our understanding of the processes involved in regulating flower development, nectar biosynthesis, and nectary development in pepper flowers. More contigs and unigenes were reported compared to previous transcriptomic studies in other plants, such as tomato (Pandurangaiah et al, 2016), potato (Cao et al, 2016), and eggplant (Ramesh et al, 2016), which indicated that pepper contains abundant gene resources. We believe that our data will help to provide important new insights and facilitate further studies on pepper genes and their functions.…”

Section: Global Gene Expression Patterns In Pepper Flowersmentioning

confidence: 85%

Flower transcriptome dynamics during nectary development in pepper (Capsicum annuum L.)

Deng

Zhao

et al. 2020

Genet. Mol. Biol.

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The measurement of gene expression can provide important information about gene function and the molecular basis for developmental processes. We analyzed the transcriptomes at three different developmental stages of pepper flower [sporogenous cell division, stage (B1); pollen mother cell meiosis, stage (B2); and open flower (B3)]. In the cDNA libraries for B1, B2, and B3: 82718, 77061, and 91491 unigenes were assembled, respectively. A total of 34,445 unigene sequences and 128 pathways were annotated by KEGG pathway analysis. Several genes associated with nectar biosynthesis and nectary development were identified, and 8,955, 12,182, and 23,667 DEGs were identified in the B2 vs B1, B3 vs B1, and B3 vs. B2 comparisons. DEGs were involved in various metabolic processes, including flower development, nectar biosynthesis, and nectary development. According to the RNA-seq data, all 13 selected DEGs showed similar expression patterns after q-PCR analysis. Sucrose-phosphatase, galactinol-sucrose galactosyltransferase, and sucrose synthase played very important roles in nectar biosynthesis, and CRABS CLAW could potentially be involved in mediating nectary development. A significant number of simple sequence repeat and single nucleotide polymorphism markers were predicted in the Capsicum annuum sequences. The new results provide valuable genetic information about flower development in pepper.

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Differential expression of carotenoid biosynthetic pathway genes in two contrasting tomato genotypes for lycopene content

Cited by 17 publications

References 21 publications

Recoloring tomato fruit by CRISPR/Cas9-mediated multiplex gene editing

Recoloring tomato fruit by CRISPR/Cas9-mediated multiplex gene editing

Molecular breeding of a novel orange-brown tomato fruit with enhanced beta-carotene and chlorophyll accumulation

Flower transcriptome dynamics during nectary development in pepper (Capsicum annuum L.)

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