Differential expression of apolipoprotein D in male reproductive system of rats by high‐fat diet

Lim, Whasun; Bae, Hyocheol; Song, Gwonhwa

doi:10.1111/andr.12250

Cited by 12 publications

(14 citation statements)

References 44 publications

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“…In the past few years, a number of miRNA have been found to alter experimentally the expression of some Lipocalins. miRNAs 299-3p, 423-3p and 490-3p were associated to ApoD expression in rat [51]; miRNAs 18b-5p, 19b-3p, 99a-5p, 100-5p, 145-5p, 214-3p and 138 alter Lcn2 expression [52,53], and miRNA 573 affects ApoM expression [54]. Some of these miRNAs were detected by the PITA algorithm [23], but they were below the ΔΔG threshold of -10 Kcal/mol to be considered accessible.…”

Section: Resultsmentioning

confidence: 99%

Characterization of mammalian Lipocalin UTRs in silico: Predictions for their role in post-transcriptional regulation

et al. 2019

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The Lipocalin family is a group of homologous proteins characterized by its big array of functional capabilities. As extracellular proteins, they can bind small hydrophobic ligands through a well-conserved β-barrel folding. Lipocalins evolutionary history sprawls across many different taxa and shows great divergence even within chordates. This variability is also found in their heterogeneous tissue expression pattern. Although a handful of promoter regions have been previously described, studies on UTR regulatory roles in Lipocalin gene expression are scarce. Here we report a comprehensive bioinformatic analysis showing that complex post-transcriptional regulation exists in Lipocalin genes, as suggested by the presence of alternative UTRs with substantial sequence conservation in mammals, alongside a high diversity of transcription start sites and alternative promoters. Strong selective pressure could have operated upon Lipocalins UTRs, leading to an enrichment in particular sequence motifs that limit the choice of secondary structures. Mapping these regulatory features to the expression pattern of early and late diverging Lipocalins suggests that UTRs represent an additional phylogenetic signal, which may help to uncover how functional pleiotropy originated within the Lipocalin family.

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Section: Resultsmentioning

confidence: 99%

Characterization of mammalian Lipocalin UTRs in silico: Predictions for their role in post-transcriptional regulation

et al. 2019

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“…The production of immature sperm is one of the main causes of male infertility. In a previous study, we identified changes in the expression of apolipoprotein D, a cholesterol-binding lipoprotein in male reproductive organs of rats fed a highcholesterol diet (Lim et al, 2016). However, no studies, to the best of our knowledge, have identified the expression of genes involved in sperm maturation and fertilization in the testes of mammals fed cholesterol.…”

Section: Discussionmentioning

confidence: 98%

Quercetin Affects Spermatogenesis-Related Genes of Mouse Exposed to High-Cholesterol Diet

Yang

Bae

Song

et al. 2020

J Anim Reprod Biotechnol

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A high-cholesterol diet can reduce male fertility. However, it is not known whether a high-cholesterol diet can regulate the expression of genes involved in sperm maturation and sperm fertilizing ability. Quercetin, a natural product, is known to have cytoprotective effects by regulating lipid metabolism in various cell types. This study aimed to confirm the expression of genes involved in sperm maturation in the testes of mice fed a high-cholesterol diet and to determine whether quercetin can reverse the genetic regulation of cholesterol. Mice were divided into groups fed a normal chow diet and a high-cholesterol diet. Mice fed the high-cholesterol diet were dose-dependently supplemented with quercetin for 6 weeks. Investigations using quantitative PCR and in situ hybridization revealed that the high-cholesterol diet alters the expression of genes associated with sperm maturation in the testes of mice, and this was reversed with the supplementation of quercetin. In addition, the high-cholesterol diet regulated the expression of genes related to lipid metabolism in the liver of mice. Under a highcholesterol diet, quercetin can improve male fertility by regulating the expression of genes involved in sperm maturation.

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“…The authors also reported that miR-423-3p targeted genes involved in fatty acid metabolism and biosynthesis pathways. The role of this miRNA in regulating lipid metabolism was shown in rats fed with a high-fat diet that resulted in increased expression of miR-423-3p and correlated inversely with the expression of apolipoprotein D [ 34 ]. Thus, this miRNA seems a regulator of fat tissue metabolism during allergic inflammation.…”

Section: Discussionmentioning

confidence: 99%

Allergic Inflammation Alters microRNA Expression Profile in Adipose Tissue in the Rat

Szczepankiewicz

Langwiński

Kołodziejski

et al. 2020

Genes

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Adipose tissue is a major source of circulating exosomal microRNAs (miRNAs) that are modulators of the immune response in various types of tissues and organs, including airways. Still, no evidence exists if allergic airway inflammation may affect fat tissue inflammation via alterations in the miRNA expression profile. Therefore, we investigated the miRNA expression profile in the adipose tissue upon induced allergic inflammation in the airways in the rat. Brown Norway rats were chronically sensitized to house dust mite extract for seven weeks. Body composition was performed using MiniSpec Plus. The eosinophil count and the total IgE level were determined to confirm the induction of allergic inflammation. MiRNA expression profiling was done using the next-generation sequencing with validation by qPCR. We found that allergic airway inflammation significantly increased fat in adipose tissue, glucose concentration, and the gene expression of adipose tissue-derived proinflammatory peptides (leptin, TNFα). In miRNA-seq analysis, we showed significant differences in the expression of 36 mature miRNAs, three precursors, and two miRNA families in adipose tissue of allergic rats. Two miRNAs—miRNA-151-5p and miRNA-423-3p—showed significantly increased expression in qPCR in adipose tissue and lungs of sensitized animals. Allergic airway inflammation affects fat tissue and alters miRNA expression profile in adipose tissue in the rat.

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Differential expression of apolipoprotein D in male reproductive system of rats by high‐fat diet

Cited by 12 publications

References 44 publications

Characterization of mammalian Lipocalin UTRs in silico: Predictions for their role in post-transcriptional regulation

Characterization of mammalian Lipocalin UTRs in silico: Predictions for their role in post-transcriptional regulation

Quercetin Affects Spermatogenesis-Related Genes of Mouse Exposed to High-Cholesterol Diet

Allergic Inflammation Alters microRNA Expression Profile in Adipose Tissue in the Rat

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