Differential enzymatic degradation of thiazole pollutants by two different peroxidases – A comparative study

Alneyadi, Aysha; Ashraf, S. Salman

doi:10.1016/j.cej.2016.06.017

Cited by 52 publications

(47 citation statements)

References 34 publications

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“…However, when 1-hydroxybenzotriazole (HOBT), one of the most commonly used redox mediators, was added to the reaction mixture, rapid and efficient SRB dye degradation was observed ( Figure 2A and Figure 2B). This absolute requirement of HOBT was not observed in the SBP-degradation of many other dyes [27,35], but is consistent with peroxidase-mediated degradation of Crystal Ponceau 6R and ThT dyes [27,36]. Optimization of HOBT concentration for efficient SRB dye degradation showed that 50 μM HOBT was the optimum amount of redox mediator for SBP-based degradation, whereas the CPO-based reaction showed the best degradation of SRB dye at 167 μM HOBT ( Figure 2B).…”

Section: The Requirement For Redox Mediator (Rm)supporting

confidence: 50%

“…We have also recently carried out a comparative study of the degradation of two different thiazole compounds using SBP and CPO enzymes. Our results showed that SBP could efficiently degrade Thioflavin T (ThT), while CPO only produced a chlorinated form of ThT without any actual degradation [27]. Additionally, SBP-degraded ThT was non-phytotoxic, whereas CPO-treated ThT was still toxic to Lactuca sativa seeds (unpublished data).…”

mentioning

confidence: 84%

“…Red and Crystal violet [39], Orange G and Sunset Yellow [40], and Thioflavin T [27], was maximum in the pH range between 2-3. Collectively, these results indicate that enzymatic remediation activity is significantly influenced by the pH of the reaction mixture, and SBP and CPO have different pH optima of pH 3-4 and pH 2-3, respectively.…”

Section: Effect Of Reaction Phmentioning

confidence: 99%

“…Additionally, SBP-degraded ThT was non-phytotoxic, whereas CPO-treated ThT was still toxic to Lactuca sativa seeds (unpublished data). In the present study, we have extended our previous study [27] by using these two related and commonly used peroxidases (SBP and CPO) to examine in detail the degradation of another aromatic dye, Sulforhodamine B (SRB dye). Although not a typical organic pollutant, Sulforhodamine B is a carcinogenic dye and has been used by scientists in the field as a model aromatic pollutant to study non-enzymatic oxidative degradation as well as adsorption from simulated waste water [29][30][31][32].…”

mentioning

confidence: 99%

“…In biological methods, agents such as microorganisms, plants or enzymes from different origins are used to degrade different classes of organic pollutants [19][20][21][22].` Enzymatic treatments, particularly peroxidases, offer the added advantage of scalability, especially when coupled with recombinant DNA technologies. A review of the literature shows that diverse peroxidases, such as soybean (SBP), manganese, lignin, chloroperoxidase (CPO), and horseradish (HRP) peroxidases have all been extensively used to efficiently degrade various classes of organic pollutants [23][24][25][26][27][28]. All of these peroxidases are heme-containing enzymes and belong to the same superfamily of heme-dependent peroxidase (E.C.…”

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confidence: 99%

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Differential Degradation and Detoxification of an Aromatic Pollutant by Two Different Peroxidases

Alneyadi

Shah

AbuQamar

et al. 2017

Preprint

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Enzymatic degradation of organic pollutants is a new and promising remediation approach.Peroxidases are one of the most commonly used classes of enzymes to degrade organic pollutants.However, it is generally assumed that all peroxidases behave similarly and produce similar degradation products. In this study, we conducted detailed studies of the degradation of a model aromatic pollutant, Sulforhodamine B dye (SRB dye), using two peroxidases-soybean peroxidase (SBP) and chloroperoxidase (CPO). Our results show that these two related enzymes had different optimum conditions (pH, temperature, H2O2 concentration...etc.) for efficiently degrading SRB dye.High-performance liquid chromatography and LC-mass spectrometry analyses confirmed that both SBP and CPO transformed the SRB dye into low molecular weight intermediates. While most of the intermediates produced by the two enzymes were the same, the CPO treatment produced at least one different intermediate. Furthermore, toxicological evaluation using lettuce (Lactuca sativa) seeds demonstrated that the SBP-based treatment was able to eliminate the phytotoxicity of SRB dye, but the CPO-based treatment did not. Our results show, for the first time, that while both of these related enzymes can be used to efficiently degrade organic pollutants, they have different optimum reaction conditions and may not be equally efficient in detoxification of organic pollutants.

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Section: The Requirement For Redox Mediator (Rm)supporting

confidence: 50%

mentioning

confidence: 84%

Section: Effect Of Reaction Phmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Differential Degradation and Detoxification of an Aromatic Pollutant by Two Different Peroxidases

Alneyadi

Shah

AbuQamar

et al. 2017

Preprint

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Bioremediation

Hussain

Farooqi

Latif

et al. 2021

Soil Bioremediation

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Aflatoxin biotransformation by commercial peroxidase and its application in contaminated food

Sibaja

Garcia

Feltrin

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND Aflatoxin B1 (AFB1) is the most commonly occurring and the most toxic of all aflatoxins (AFs). It is responsible for liver cancer in animals and it is listed as a Group I carcinogen by the International Agency for Research on Cancer. Thus, it is necessary to find decontamination methods that show efficiency, specificity, low cost, and that allow the disposal of contaminated raw materials into the environment. This study evaluated the effects of enzyme concentration, pH, temperature and reaction time on AFB1 biotransformation by commercial peroxidase (POD) in a model solution (100 mmol L−1 phosphate buffer). RESULTS When 0.015 U mL−1 was used to treat 0.5 µg L−1 of AFB1, at pH 7.0–8.0 and 30–40 °C, for 8.0 h, the AFB1 biotransformation reached its maximum (97%). KM value of POD for the biotransformation of AFB1 was 0.0160 µmol L−1 (5 µg L−1) with a VMAX of 6.4 µmol L−1 min−1, which were determined based on Lineweaver‐Burk's plot. POD (0.015 U mL−1) effectively reduces AFB1 (97%) and M1 (65%) in UHT milk and AFB1 (24%) in lager beer samples acquired in markets of Rio Grande‐RS (Brazil). CONCLUSION The use of POD may be a strategy to mitigate the impact of AFs found in food to reduce the risk of consumer exposure. © 2018 Society of Chemical Industry

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Differential enzymatic degradation of thiazole pollutants by two different peroxidases – A comparative study

Cited by 52 publications

References 34 publications

Differential Degradation and Detoxification of an Aromatic Pollutant by Two Different Peroxidases

Differential Degradation and Detoxification of an Aromatic Pollutant by Two Different Peroxidases

Bioremediation

Aflatoxin biotransformation by commercial peroxidase and its application in contaminated food

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