Differential effects of chemoattractants on mast cell recruitment in vivo

Abstract

Rats were injected with rat recombinant (rr) IL3, rrSCF, rrIL-3+rrSCF, rrRANTES and LTB4. Six hours after subcutaneous injection of rrIL-3 or rrIL-3+rrSCF, there was an increase in mast cell numbers in the skin and spleen. Peritoneal mast cells were recruited following i.p. injection of rrIL-3, but with rrIL-3+rrSCF recruitment was delayed. Immunostaining with a mast cell specific antibody showed that immature orthochromatic mast cells were being recruited. rrIL-3 induced recruitment of mast cells to the peritoneal cavity was blocked by anti-integrin antibodies. Mast cell recruitment depended on the target tissue and the time of exposure to the chemoattractant.

Mast Cell Function

“…Mac-1, a β2 integrin important for leukocyte migration, has been shown to be required for maintenance of mast cell levels in the peritoneal cavity, peritoneal wall, and certain regions of the skin. Mast cell recruitment to the peritoneal cavity in response to rat recombinant (rr)IL-3 was significantly inhibited by a prior intraperitoneal injection of antibodies against the integrin subunits α4 and β7 ( de Cássia Campos et al 2014 ). The αIIbβ3 integrin has a role in the adhesion of BMMCs to different substrates and influences the homing of mast cell progenitors to the peritoneal cavity ( Rosenkranz et al 1998 ; Berlanga et al 2005 ).…”

“…In contrast, antigen-stimulated BMMCs migrated in response to MCP-1, RANTES, macrophage inflammatory protein-1alpha (MIP-1α or CCL3), and platelet factor-4 (PF4 or CXCL4) ( Taub et al 1995 ). Subcutaneous injection of RANTES induced an increase in the number of metachromatic mast cells in the dermis and the spleen of Wistar rats ( de Cássia Campos et al 2014 ). Although, mast cells express several chemokine receptors and many chemokines have been shown to be chemoattractants for mast cells in vitro, no mast cell-specific chemokine has been described ( Taub et al 1995 ; Collington et al 2011 ).…”

“…In patients with severe asthma, chymase-positive mast cells were increased in small airway regions and correlated positively with lung function ( Balzar et al 2005 ). The lipid mediator leukotriene B4 (LTB4) was shown to be important in the recruitment of mast cell progenitors to inflamed tissues and the subcutaneous injection of LTB4 induced an increase in the number of metachromatic mast cells in both the dermis and the spleen of Wistar rats ( Weller et al 2005 ; de Cássia Campos et al 2014 ). In addition, Transforming Growth Factor-β (TGF-β), also released by mast cells, was shown to be a potent chemoattractant for mast cells in vitro ( Gruber et al 1994 ; Olsson et al 2000 ; Lindstedt et al 2001 ; Olsson et al 2001 ).…”

The Perfect Storm Review: Immune Dysregulation in Severe COVID-19 and the Possible Role of Mast Cell-Vitamin D Interactions

“…Peptides that caused vasoconstriction and bound to MRGPRX2 were Sarafotoxin B venom and the microbial cathelicidin-associated LL-37. There were differential effects, dependant on the target tissue and time of exposure to the chemoattractant [28,29]. Other mediators that stimulate MCs and trigger degranulation, proliferation and release of mediators include, IgG, complement components, TLR ligands, neuropeptides, cytokines, chemokines as well as other inflammatory products [28][29][30].…”

SAMSN1: proteína adaptadora essencial na função de mastócitos

“…A subunidade 2 de integrina também é importante para o homing de mastócitos para a pele (Rosenkranz et al, 1998) (Collington et al, 2011). Integrinas, moléculas de adesão, quimiocinas e seus receptores, bem como citocinas e fatores de crescimento são agentes importantes na migração direcionada de progenitores de mastócitos para locais específicos, tanto em situações fisiológicas como patológicas (Gurish et al, 2001; J. Pablo Abonia, K. Frank Austen, Barrett J. Rollins, Sunil K. Joshi, Richard A. Flavell, William A. Kuziel, Pandelakis A. Koni, 2005;Rosenkranz et al, 1998;Collington et al, 2010b;de Cássia Campos et al, 2014).…”