Differential effects of activin-A and FSH on growth, viability and messenger RNA expression in cultured bovine preantral follicles

Silva, A.W.B.; Bezerra, F.T.G.; Costa, Jonathan L.; Rossi, R.O.D.S.; Passos, M. J.; Vasconcelos, G. L. de; Rossetto, R.; Donato, Mariana Aragão Matos; Magalhães-Padilha, D.M.; Campello, C.C.; Saraiva, M.V.A.; Figueiredo, J.R.; Peixoto, Christina Alves; Hurk, R. van den; Silva, J.R.V.

doi:10.1016/j.livsci.2013.12.003

Cited by 5 publications

(2 citation statements)

References 29 publications

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“…Although we identified multiple roles for activin in the dog system, it was somewhat unexpected that activin had no discernible effect on the preantral follicle, especially as activin A is known to promote growth of these same stage follicles in the cow [55]. This hormone clearly was not the primary driver for initiating antrum development in the dog.…”

Section: Discussionmentioning

confidence: 91%

“…activin also appears to promote FSH receptor mRNA expression in granulosa cells [37,38,59], a mechanism that we did not observe in the dog. As activin also has been postulated to block the stimulatory effects of FSH on early stage preantral follicles in the cow [55] and rat [58], it would be useful to examine the influence of this hormone only on this stage dog follicle before FSH exposure.…”

Section: Discussionmentioning

confidence: 99%

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Activin promotes growth and antral cavity expansion in the dog ovarian follicle

et al. 2019

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Understanding regulators of folliculogenesis remains limited in the domestic dog (Canis familiaris), which challenges our ability to develop in vitro follicle culture systems for canid genome rescue efforts. Here, we investigated the influence of activin on dog follicle development and survival, oocyte quality, and FSH receptor expression in culture. Preantral (150-≤230 µm diameter), early antral (231-≤330 µm), and antral (>330-550 µm) stage follicles were encapsulated in a fibrin-alginate hydrogel with 0, 100, or 200 ng/ml rhActivin plus 0, 0.1, 1, or 10 µg/ml FSH for 12 or 21 d of in vitro culture. All follicle groups increased in diameter (P < 0.05) with activin acting synergistically with FSH to improve (P < 0.05) growth and antral cavity expansion (to >630 µm) in early antral and antral cohorts. This complementary effect was not linked to changes in FSHR mRNA expression (P > 0.05). Although not influencing (P > 0.05) follicle survival or transzonal projection (TZP) density in shorter term 12 d culture, activin in the presence of 1 ng/ml FSH maintained TZP density from the 12 to 21 d interval. Activin also increased oocyte diameter and improved nuclear integrity compared to un-supplemented controls. These results indicate that activin acts synergistically with FSH to promote growth and antral cavity expansion of the dog follicle in vitro, information useful to formulating an effective culture microenvironment for this species.

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Section: Discussionmentioning

confidence: 91%