Differential Distribution and Expression of Panton-Valentine Leucocidin among Community-Acquired Methicillin-Resistant
            <i>Staphylococcus aureus</i>
            Strains

Saïd-Salim, Battouli; Mathema, Barun; Braughton, Kevin R.; Davis, Stacy; Sinsimer, Daniel; Eisner, William; Likhoshvay, Yekaterina; DeLeo, Frank R.; Kreiswirth, Barry N.

doi:10.1128/jcm.43.7.3373-3379.2005

Cited by 103 publications

(72 citation statements)

References 31 publications

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“…One of our strains, 03-1816, also showed a prolonged expression of luk-PV into the post-exponential phase. Overall, the time-course and the level of luk-PV expression seem to vary considerably between different S. aureus strains, as was also shown by other groups (Hamilton et al, 2007;Said-Salim et al, 2005). Strains MW2 and USA300 in particular differed in the level of PVL expression.…”

Section: Influence Of Global Virulence Regulators On Luk-pv Transcripsupporting

confidence: 73%

Transcription of the phage-encoded Panton–Valentine leukocidin of Staphylococcus aureus is dependent on the phage life-cycle and on the host background

et al. 2009

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Panton-Valentine leukocidin (PVL) is a pore-forming, bi-component toxin secreted by Staphylococcus aureus strains epidemiologically associated with diseases such as necrotizing pneumonia and skin and soft-tissue infections. Here we demonstrate that transcription of the phage-encoded PVL (encoded in the luk-PV operon) is dependent on two major determinants: the phage life-cycle and the host chromosomal background. Mitomycin C induction of PVLencoding prophages from different community-acquired MRSA strains led to an increase in the amount of luk-PV mRNA as a result of read-through transcription from latent phage promoters and an increase in phage copy numbers. Failing prophage excision was reflected in a constant expression of luk-PV as in the case of strain USA300, suggesting that wSa2USA300 is a replication-defective prophage. Additionally, we could show that luk-PV transcription is influenced by the S. aureus global virulence regulators agr and sae. We found a strong impact of the host background on prophage induction and replication when analysing PVL phages in different S. aureus strains. For example phage wSa2mw was greatly induced by mitomycin C in its native host MW2 and in strain Newman but to a considerably lesser extent in strains 8325-4, RN6390 and ISP479c. This discrepancy was not linked to the SOS response of the bacteria since recA transcription did not vary between the strains. These results suggest a fine tuning between certain phages and their host, with major impact on the expression of phage-encoded virulence genes. INTRODUCTIONStaphylococcus aureus causes a variety of local and systemic infections in humans and is one of the most important community-acquired and nosocomial pathogens. The versatility of this bacterium is due to its ability to produce a wide range of surface-exposed molecules which mediate interaction with the host cell, as well as several secreted virulence factors. S. aureus necrotizing pneumonia and skin and soft-tissue infections, which can also affect young, immunocompetent persons, is described as a threat associated with community-acquired (ca)MRSA and MSSA strains bearing the Panton-Valentine leukocidin (PVL) genes (Diederen & Kluytmans, 2006;Gillet et al., 2002;Vandenesch et al., 2003). The possible contribution of PVL to the virulence of S. aureus has been argued in studies using a variety of different animal models (Bubeck Wardenburg et al., 2008;Diep & Otto, 2008; LabandeiraRey et al., 2007;Montgomery et al., 2008;Voyich et al., 2006;Wang et al., 2007). PVL is a bi-component, poreforming cytotoxin that targets host defence cells such as human polymorphonuclear neutrophils, monocytes and macrophages (Genestier et al., 2005;Kaneko & Kamio, 2004;Prevost et al., 1995). The active form of PVL requires the assembly of two polypeptides, LukS-PV and LukF-PV, for which the corresponding genes (lukS-PV, lukF-PV) are carried by a prophage.Although tightly linked to the phage genome and dependent on it for horizontal transfer, most of the phage-encoded virulence factors are i...

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Section: Influence Of Global Virulence Regulators On Luk-pv Transcripsupporting

confidence: 73%

Transcription of the phage-encoded Panton–Valentine leukocidin of Staphylococcus aureus is dependent on the phage life-cycle and on the host background

et al. 2009

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“…The pvl, icaA, icaD, IS256, agr, bap, and fnbB genes were assessed with endpoint PCR according to methods described by Saïd-Salim et al (5) , Martin-Lopez et al (6) , Arciola et al (7) , Gu et al (8) , Shopsin et al (9) , Cucarella et al (10) and Tristan et al (11) . The oligonucleotide primers described previously by Kondo et al (12) were used for verifying the various types of SCCmec elements.…”

mentioning

confidence: 99%

The nares as a CA-MRSA reservoir in the healthy elderly

Brito

Queiroz

Campos

et al. 2015

Rev. Soc. Bras. Med. Trop.

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“…The presence in the lungs causes hemorrhage, extensive necrosis of alveolar septa, destruction of the epithelium covering the bronchi and bronchioles (Zhang et al, 2005), and histological sections show necrotic lesions in the mucosa of the trachea (Lina et al, 1999). Due to these facts, studies have proposed that the propensity of CA-MRSA infections cause severe skin and soft tissue lesions, and possibly necrotizing pneumonia, is due to the presence of the gene encoding the production of PVL (Saïd-Salim et al 2005). …”

Section: The Role Of the Panton-valentine Leukocidin (Pvl)mentioning

confidence: 99%

“…The acquisition of genes encoding PVL is made by transduction of a specific type of bacteriophage, phiSLT, which causes cytolysis in carrier gene cells and transport this gene to another cell. From its transcription two exoproteins , the Luks-PV and LukF-PV are produced, acting through the synergistic action of both subunits (Melles et al, 2006, Saïd-Salim et al, 2005. When secreted, LukS-PV initiates a connection to the membrane of the polymorphonuclear leukocyte (PMN) and is dimerized with LukF-PV, alternating one and another until the complete formation of a heptamer.…”

Section: The Role Of the Panton-valentine Leukocidin (Pvl)mentioning

confidence: 99%