2010
DOI: 10.1186/1465-9921-11-124
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Abstract: BackgroundInvestigations on pulmonary macrophages (MΦ) mostly focus on alveolar MΦ (AM) as a well-defined cell population. Characteristics of MΦ in the interstitium, referred to as lung interstitial MΦ (IM), are rather ill-defined. In this study we therefore aimed to elucidate differences between AM and IM obtained from human lung tissue.MethodsHuman AM and IM were isolated from human non-tumor lung tissue from patients undergoing lung resection. Cell morphology was visualized using either light, electron or c… Show more

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Cited by 83 publications
(101 citation statements)
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“…Interestingly, although IMs express higher levels of IL-10 than AMs [5,19] and IL-10 induces GILZ expression [14], expression levels do not differ between AMs and IMs. The transcript variants of GILZ described in the literature have as yet only been reported for the murine system [29,30].…”
Section: Discussionmentioning
confidence: 87%
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“…Interestingly, although IMs express higher levels of IL-10 than AMs [5,19] and IL-10 induces GILZ expression [14], expression levels do not differ between AMs and IMs. The transcript variants of GILZ described in the literature have as yet only been reported for the murine system [29,30].…”
Section: Discussionmentioning
confidence: 87%
“…RNA isolation and reverse transcription were performed as described previously [19,48]. Primer and probe sequences for realtime RT-PCR are given in Tables 1 and . Cloned standards were run alongside the samples to generate a standard curve.…”
Section: Semiquantitative Rt-pcr and Real-time Rt-pcrmentioning
confidence: 99%
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“…The informed consent of all participating subjects was obtained. Isolation was performed according to a previously described method (24). After visible bronchi were removed, the lung tissue was chopped and washed with balanced salt solution (137 mM NaCl, 5 mM KCl, 0.7 mM Na 2 HPO 4 , 10 mM HEPES, 5.5 mM glucose, pH 7.4).…”
Section: /2013)mentioning
confidence: 99%
“…Staining was essentially performed as described previously (24,25). In brief, cells were detached from the plates in TEN buffer (40 mM Tris, 1 mM EDTA, 150 mM NaCl) before staining.…”
Section: Flow Cytometrymentioning
confidence: 99%