Differential apoptosis-related protein expression, mitochondrial properties, proteolytic enzyme activity, and DNA fragmentation between skeletal muscles

McMillan, Elliott M.; Quadrilatero, Joe

doi:10.1152/ajpregu.00488.2010

Cited by 42 publications

(50 citation statements)

References 73 publications

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“…17,35 Briefly, equal protein was loaded on 12% C. Membranes were then washed with TBS-T and incubated with the appropriate horseradish peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology) for 1 h at room temperature. Immunoreactive bands were visualized using Clarity Western ECL Substrate (BioRad Laboratories; Hercules, CA) or ECL Western Blot Substrate (BioVision; Milpitas, CA) and the ChemiGenius 2 Bio-Imaging System (Syngene, Cambridge, UK).…”

Section: Immunoblot Analysesmentioning

confidence: 99%

“…17 Absorbance was measured at 405 nm and 490 nm using a SPECTRAmax Plus spectrophotometer (Molecular Devices; Sunnyvale, CA). A positive control for DNA fragmentation (DNA-histone-complex) was included with each assay.…”

Section: Dna Fragmentationmentioning

confidence: 99%

“…Caspase-3, caspase-8, caspase-9, and cathepsin enzymatic activity in muscle homogenates was determined as previously described 17 using the substrates Ac-DEVD-AMC (Enzo Life Sciences), Ac-IETD-AMC (Sigma-Aldrich), Ac-LEHD-AMC (Enzo Life Sciences), and z-FR-AFC (Enzo Life Sciences), respectively. Briefly, samples were homogenized in ice-cold lysis buffer without protease inhibitors using a glass homogenizer and incubated in duplicate at room temperature (in the dark) with the appropriate substrate.…”

Section: Caspase and Cathepsin Enzyme Activitymentioning

confidence: 99%

“…Although this study investigated morphological (extensor digitorum longus and soleus), apoptotic (tibialis anterior), and autophagic (mixed gastrocnemius) indices, these analyses were performed across different muscles. Given that there are fiber/muscle differences in apoptotic 17 as well as autophagic 18,19 signaling and protein expression, a concurrent evaluation of these pathways within skeletal muscles of different fiber composition would provide novel insight regarding this relationship following HF feeding. Additionally, despite evidence that some physiological responses to HF feeding are sex-dependent, [20][21][22] much of the current literature has been conducted using male animals.…”

Section: Introductionmentioning

confidence: 99%

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High-fat feeding does not induce an autophagic or apoptotic phenotype in female rat skeletal muscle

Campbell

Mitchell

McMillan

et al. 2014

Exp Biol Med (Maywood)

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Apoptosis and autophagy are critical in normal skeletal muscle homeostasis; however, dysregulation can lead to muscle atrophy and dysfunction. Lipotoxicity and/or lipid accumulation may promote apoptosis, as well as directly or indirectly influence autophagic signaling. Therefore, the purpose of this study was to examine the effect of a 16-week high-fat diet on morphological, apoptotic, and autophagic indices in oxidative and glycolytic skeletal muscle of female rats. High-fat feeding resulted in increased fat pad mass, altered glucose tolerance, and lower muscle pAKT levels, as well as lipid accumulation and reactive oxygen species generation in soleus muscle; however, muscle weights, fiber type-specific cross-sectional area, and fiber type distribution were not affected. Moreover, DNA fragmentation and LC3 lipidation as well as several apoptotic (ARC, Bax, Bid, tBid, Hsp70, pBcl-2) and autophagic (ATG7, ATG4B, Beclin 1, BNIP3, p70 s6k, cathepsin activity) indices were not altered in soleus or plantaris following high-fat diet. Interestingly, soleus muscle displayed small increases in caspase-3, caspase-8, and caspase-9 activity, as well as higher ATG12-5 and p62 protein, while both soleus and plantaris muscle showed dramatically reduced Bcl-2 and X-linked inhibitor of apoptosis protein (XIAP) levels. In conclusion, this work demonstrates that 16 weeks of high-fat feeding does not affect tissue morphology or induce a global autophagic or apoptotic phenotype in skeletal muscle of female rats. However, high-fat feeding selectively influenced a number of apoptotic and autophagic indices which could have implications during periods of enhanced muscle stress.

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Section: Immunoblot Analysesmentioning

confidence: 99%

Section: Dna Fragmentationmentioning

confidence: 99%

Section: Caspase and Cathepsin Enzyme Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

High-fat feeding does not induce an autophagic or apoptotic phenotype in female rat skeletal muscle

Campbell

Mitchell

McMillan

et al. 2014

Exp Biol Med (Maywood)

Self Cite

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show abstract

“…In rodent skeletal muscle, expression of µ-calpain is not dependent on muscle fiber type 64) . Recently, however, McMillan and Quadrilatero 65) determined that caspase-3, -8, and -9 activity, calpain activity, and number of apoptotic cells were also significantly higher in the red compared with white gastrocnemius. In contrast, Murphy and Lamb 66) have shown that calpain-3 activity was ~1.9 times higher in individual muscle fiber segments from extensor digitorum longus (EDL) muscle compared to soleus muscle.…”

Section: Intracellular Ca 2+ and Muscle Damagementioning

confidence: 98%

Mechanisms of exercise-induced muscle damage and fatigue: Intracellular calcium accumulation

Kano

Sonobe

Sudo

et al. 2012

JPFSM

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Diaphragm assessment in mice overexpressing phospholamban in slow‐twitch type I muscle fibers

et al. 2016

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AimsPhospholamban (PLN) and sarcolipin (SLN) are small inhibitory proteins that regulate the sarco(endo)plasmic reticulum Ca2+‐ATPase (SERCA) pump. Previous work from our laboratory revealed that in the soleus and gluteus minimus muscles of mice overexpressing PLN (Pln OE), SERCA function was impaired, dynamin 2 (3–5 fold) and SLN (7–9 fold) were upregulated, and features of human centronuclear myopathy (CNM) were observed. Here, we performed structural and functional experiments to evaluate whether the diaphragm muscles of the Pln OE mouse would exhibit CNM pathology and muscle weakness.MethodsDiaphragm muscles from Pln OE and WT mice were subjected to histological/histochemical/immunofluorescent staining, Ca2+‐ATPase and Ca2+ uptake assays, Western blotting, and in vitro electrical stimulation.ResultsOur results demonstrate that PLN overexpression reduced SERCA's apparent affinity for Ca2+ but did not reduce maximal SERCA activity or rates of Ca2+ uptake. SLN was upregulated 2.5‐fold, whereas no changes in dynamin 2 expression were found. With respect to CNM, we did not observe type I fiber predominance, central nuclei, or central aggregation of oxidative activity in diaphragm, although type I fiber hypotrophy was present. Furthermore, in vitro contractility assessment of Pln OE diaphragm strips revealed no reductions in force‐generating capacity, maximal rates of relaxation or force development, but did indicate that ½ relaxation time was prolonged.ConclusionsTherefore, the effects of PLN overexpression on skeletal muscle phenotype differ between diaphragm and the postural soleus and gluteus minimus muscles. Our findings here point to differences in SLN expression and type I fiber distribution as potential contributing factors.

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Differential apoptosis-related protein expression, mitochondrial properties, proteolytic enzyme activity, and DNA fragmentation between skeletal muscles

Abstract: McMillan EM, Quadrilatero J. Differential apoptosis-related protein expression, mitochondrial properties, proteolytic enzyme activity, and DNA fragmentation between skeletal muscles.

Cited by 42 publications

References 73 publications

High-fat feeding does not induce an autophagic or apoptotic phenotype in female rat skeletal muscle

High-fat feeding does not induce an autophagic or apoptotic phenotype in female rat skeletal muscle

Mechanisms of exercise-induced muscle damage and fatigue: Intracellular calcium accumulation

Diaphragm assessment in mice overexpressing phospholamban in slow‐twitch type I muscle fibers

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