Different sensitivity of human endothelial cells, smooth muscle cells and fibroblasts to topography in the nano–micro range

Biela, Sarah; Su, Yi; Spatz, Joachim P.; Kemkemer, Ralf

doi:10.1016/j.actbio.2009.04.003

Cited by 269 publications

(280 citation statements)

References 38 publications

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“…MC3T3-E1 preosteoblasts (American Type Culture Collection, Manassas, VA, USA) were cultured in aMEM with deoxyribonucleosides, ribonucleosides and 2 mM L-glutamine (Gibco Life Technologies, Grand Island, NY, USA), supplemented with 10% fetal bovine serum (Gibco), penicillin (100 units ml 21 ) and streptomycin (100 mg ml 21 ). The cells were incubated at 378C in a humidified atmosphere of 95% air and 5% CO 2 , and the culture medium was changed every 2 days.…”

Section: Cell Culturementioning

confidence: 99%

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Topographic cell instructive patterns to control cell adhesion, polarization and migration

Ventre

Natale

Rianna

et al. 2014

J. R. Soc. Interface.

105

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Topographic patterns are known to affect cellular processes such as adhesion, migration and differentiation. However, the optimal way to deliver topographic signals to provide cells with precise instructions has not been defined yet. In this work, we hypothesize that topographic patterns may be able to control the sensing and adhesion machinery of cells when their interval features are tuned on the characteristic lengths of filopodial probing and focal adhesions (FAs). Features separated by distance beyond the length of filopodia cannot be readily perceived; therefore, the formation of new adhesions is discouraged. If, however, topographic features are separated by a distance within the reach of filopodia extension, cells can establish contact between adjacent topographic islands. In the latter case, cell adhesion and polarization rely upon the growth of FAs occurring on a specific length scale that depends on the chemical properties of the surface. Topographic patterns and chemical properties may interfere with the growth of FAs, thus making adhesions unstable. To test this hypothesis, we fabricated different micropatterned surfaces displaying feature dimensions and adhesive properties able to interfere with the filopodial sensing and the adhesion maturation, selectively. Our data demonstrate that it is possible to exert a potent control on cell adhesion, elongation and migration by tuning topographic features' dimensions and surface chemistry.

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Section: Cell Culturementioning

confidence: 99%

“…Directionality was assessed by evaluating the total movement of each cell along and across pattern direction as described by Biela et al [21]. Briefly, let x be the pattern direction, then the directional index is defined as the ratio of the quantities defined as …”

Section: Time-lapse Experimentsmentioning

confidence: 99%

Topographic cell instructive patterns to control cell adhesion, polarization and migration

Ventre

Natale

Rianna

et al. 2014

J. R. Soc. Interface.

105

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show abstract

“…[14][15][16][17]19,21,22,24,25,59,60 By using silicon curved cavities, different types of adhesion have been demonstrated between fibroblasts ( Fig. 2A, B) and malignant cancer cells (Fig.…”

mentioning

confidence: 99%

“…Such inconsistency is in part due to the biological complexity: different cell types have been used in different studies, although the response of cells to ECM topography is known to vary depending on a cell type. 4,[14][15][16][17][18][19][20][21][22][23][24][25] In this review article, we propose a design concept of the topographical features of the synthetic ECM niche by shedding light on the actin cytoskeleton. First, we present a Biophysical Basis for designing ECM micro/nanotopography by explaining the Mechanism via Which Cells Sense and Respond to ECM Topography.…”

mentioning

confidence: 99%

Topography Design Concept of a Tissue Engineering Scaffold for Controlling Cell Function and Fate Through Actin Cytoskeletal Modulation

Miyoshi

Adachi

2014

Tissue Engineering Part B: Reviews

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“…This has since evolved to see the design of a range of surface features including islands [7], pits [23], grooves [1] and pillars [11], all of which have been shown to have an effect on cell behaviour. Such design features have been incorporated into the development of biomedical implant technology.…”

Section: Introductionmentioning

confidence: 99%

Machine grinding as an alternative method for creating functional surfaces for controlling cell behaviour

Irving

Murphy

Morgan

et al. 2016

Int J Adv Manuf Technol

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There is extensive evidence to show that certain cellular behaviours including cell proliferation, migration and adhesion can be controlled by culturing cells on surfaces containing different micro-metre-and nanometre-scale features. This paper will introduce the use of machine grinding to generate surfaces with micro-sized features and their ability to affect cell behaviour. Results are presented which show that polyurethane castings of the ground surfaces can promote cell adhesion and migration. This study demonstrates the usefulness of surface grinding as a cost-effective method for generating functional surfaces for modifying cell behaviour.

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Different sensitivity of human endothelial cells, smooth muscle cells and fibroblasts to topography in the nano–micro range

Cited by 269 publications

References 38 publications

Topographic cell instructive patterns to control cell adhesion, polarization and migration

Topographic cell instructive patterns to control cell adhesion, polarization and migration

Topography Design Concept of a Tissue Engineering Scaffold for Controlling Cell Function and Fate Through Actin Cytoskeletal Modulation

Machine grinding as an alternative method for creating functional surfaces for controlling cell behaviour

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