Different forms of chronic childhood thrombocytopenic purpura defined by antiplatelet autoantibodies

Imbach, Paul; Tani, P; Berchtold, Willi; Blanchette, V.; Burek-Kozlowska, Anna; Gerber, H; Jacobs, Peter; Newland, A. C.; Turner, Chris; Wood, Laurence J.; McMillan, Robert

doi:10.1016/s0022-3476(05)83373-6

Cited by 34 publications

(25 citation statements)

References 11 publications

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“…In the latter subjects, thrombocytopenia is likely 'compensat ed', and they may be at risk of relapse in spite of their appar ent long-term remission [5]. Alternatively, the finding of antiplatelet antibodies during remission may be predictive of an evolution towards a systemic autoimmune illness.…”

Section: Discussionmentioning

confidence: 99%

“…The onset is typically abrupt, often after a viral illness, and plate Antibodies against platelet membrane glycoprotein lib/ Ilia, Ib/IX and Ia/IIa can be detected in many ITP patients [3][4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

“…The detection of circulating platelet-reactive antibodies, although not mandatory, may support the diagnosis of ITP at onset [1] and represents a marker of residual disease in chronic patients who already attained platelet count normal ization [1,5], Several methods have been developed for routine diag nostic detection of platelet antibodies. Unfortunately, serum titer of antibodies is often low, and in many cases, the only antibodies that can be detected are those bound to the plate let surface [11], However, detection of platelet-bound anti bodies is difficult to carry out when platelet count is very low, as it is at the onset of ITP.…”

Section: Introductionmentioning

confidence: 99%

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Platelet Antibody Detection in Pediatric Immune Thrombocytopenic Purpura: Evaluation of Three Screening Methods

Biglino¹,

Perutelli²,

Mori³

1997

Vox Sanguinis

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Background and objectives: Immune thrombocytopenic purpura (ITP) is a common hematologic disorder, two forms of which occur in children. The detection of circulating platelet antibodies is helpful in diagnosis. Materials and methods: We evaluated three different immunological methods for detecting platelet antibodies in the serum of children with ITP. These were: a solid-phase red-cell adherence test (SPRCA), an enzyme immunoassay (EIA), and an immunofluorescence test (PSIF). Results: The sensitivity of the methods in detecting IgG antibodies ranged from 28.1 (EIA) to 39.4% (SPRCA). We also looked for IgM antibodies by PSIF, thus raising the sensitivity of this test from 32.0 to 40.0%. A combination of two tests (SPRCA and EIA) allowed us to detect 61.8% positive samples. By doing all three tests, we obtained 71.3% positive samples. Finally, we reached 73.5% by adding PSIF for IgM. We found a higher frequency of circulating antibodies in both acute and chronic ITP at onset than in clinical remission. There were a few positive sera in chronic ITP, but not in the acute form in remission. Conclusion: The individual tests each have a relatively low sensitivity, but the combination of all three increases the diagnostic effectiveness. The finding of platelet antibodies during remission may predict evoluation toward a systemic autoimmune state.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Platelet Antibody Detection in Pediatric Immune Thrombocytopenic Purpura: Evaluation of Three Screening Methods

Biglino¹,

Perutelli²,

Mori³

1997

Vox Sanguinis

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“…Nevertheless, the detection of direct platelet eluates requires a selected patient population with a relatively high platelet count. Immunobead assay Disease-related TP 19 9 (47) 2 (11) Berchtold et al, 1989c Immunoprecipitation ITP 6 4 Tomiyama et al, 1990 Immunobead assay ITP children ITP adults 41 16 40 (98) 14 (88) 1 (2) 2 (13) Imbach et al, 1991 MAIPA Immune TP 29 21 (72) 22 (28) 14 (48) Kiefel et al, 1991 ELISA ITP 71 25 (35) 10 (14) Kokawa et al, 1991 Immunobead assay ITP In remission 53 17…”

Section: Detection Of Gp-specific Pa-iggmentioning

confidence: 99%

Autoimmune Thrombocytopenia

2017

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“…Antibodies against platelet membrane glycoprotein IIb/ IIIa, Ib/IX and Ia/IIa can be detected in many ITP patients [3][4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Platelet Antibody Detection in Pediatric Immune Thrombocytopenic Purpura: Evaluation of Three Screening Methods

1997

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Different forms of chronic childhood thrombocytopenic purpura defined by antiplatelet autoantibodies

Cited by 34 publications

References 11 publications

Platelet Antibody Detection in Pediatric Immune Thrombocytopenic Purpura: Evaluation of Three Screening Methods

Platelet Antibody Detection in Pediatric Immune Thrombocytopenic Purpura: Evaluation of Three Screening Methods

Autoimmune Thrombocytopenia

Platelet Antibody Detection in Pediatric Immune Thrombocytopenic Purpura: Evaluation of Three Screening Methods

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