Different expression of 25-kDa heat-shock protein (Hsp25) in Meckel's cartilage compared with other cartilages in the mouse

Shimada, Masumi; Yamamoto, Miyuki; Wakayama, Tomohiko; Iseki, Shoichi; Amano, Osamu

doi:10.1007/s00429-002-0297-y

Cited by 18 publications

(18 citation statements)

References 43 publications

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“…Also, using in vivo tissue, Hsp27 expression has been reported in various tissues accompanying the cell differentiation such as epiphyseal plate cartilage (Tiffee et al 2000), Meckel's cartilage (Shimada et al 2003), stratifi ed squamous epithelium (Laplante et al 1998;Wakayama and Iseki 1998) and testis (Wakayama and Iseki 1999).…”

Section: Discussionmentioning

confidence: 99%

Expression of Heat Shock Protein 27 with the Transition from Proliferation to Differentiation of Acinar Precursor Cell in Regenerating Submandibular Gland of Rats

Takahashi-Horiuchi

Sugiyama

Sakashita

et al. 2008

Tohoku J. Exp. Med.

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Section: Discussionmentioning

confidence: 99%

Expression of Heat Shock Protein 27 with the Transition from Proliferation to Differentiation of Acinar Precursor Cell in Regenerating Submandibular Gland of Rats

Takahashi-Horiuchi

Sugiyama

Sakashita

et al. 2008

Tohoku J. Exp. Med.

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“…However, portions of Meckel's cartilage are reported to be in terminological confusion (Bhaskar et al. 1953; Bernick & Patek, 1969; Frommer & Margolies, 1971; Asling, 1972; Savostin‐Asling & Asling, 1973; Harada & Ishizeki, 1998; Shimada et al. 2003).…”

Section: Resultsmentioning

confidence: 99%

“…1999). A series of these phenomena are frequently noted in endochondral ossification, but there has been controversy as to whether the middle portion of Meckel's cartilage contributes to mandibular bone formation by endochondral ossification (Frommer & Margolies, 1971; Melcher, 1972; Savostin‐Asling & Asling, 1973; Shimada et al. 2003).…”

Section: Introductionmentioning

confidence: 99%

Immunolocalization of receptor activator of nuclear factor‐κB ligand (RANKL) and osteoprotegerin (OPG) in Meckel's cartilage compared with developing endochondral bones in mice

Sakakura¹,

Tsuruga²,

Irie³

et al. 2005

Journal of Anatomy

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We examined the immunolocalization of receptor activator of nuclear factor-κ B ligand (RANKL) and osteoprotegerin (OPG) in areas of resorption caused by osteoclasts/chondroclasts on embryonic days 14-16 (E14-16) in Meckel's cartilage, and compared the results with those in endochondral bones in mice. Intense RANKL and OPG immunoreactivity was detected in the chondrocytes in Meckel's cartilage. On E15, when the incisor teeth were closest to the middle portion of Meckel's cartilage, tartrate-resistant acid phosphatase (TRAP)-positive cells appeared on the lateral side of the cartilage. Furthermore, the dental follicle showed moderate immunoreactivity for RANKL and OPG, whereas osteoblasts derived from perichondral cells were immunonegative for RANKL and OPG in that area.On E16, cartilage resorption by TRAP-positive cells had progressed at the differential position, and intensely immunoreactive products of RANKL were overlapped on and found to exist next to TRAP-positive cells in the resorption area. In developing metatarsal tissue, OPG immunoreactivity was intense in periosteal osteoblasts, whereas RANKL was only faintly seen in some of the periosteal cells. In epiphyseal chondrocytes of the developing femur, RANKL immunoreactivity was moderate, and OPG scarcely detected. These results indicate a peculiarity of RANKL and OPG immunolocalization in resorption of Meckel's cartilage. Growth of the incisor teeth may be involved in the timeand position-specific resorption of Meckel's cartilage through local regulation of the RANKL/OPG system in dental follicular cells and periosteal osteoblasts, whereas RANKL and OPG in chondrocytes seem to contribute to resorption through regulation of the chondroclast function.

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“…Heat-shock protein (Hsp)-25 is reportedly expressed during organogenesis, including in the development processes of muscle (Gernold et al 1993), nerve (Calabrese et al 2002;Read et al 2008), cartilage (Shimada et al 2003), the temporomandibular joint (Ikeda et al 2004), skin (Duverger et al 2004), and teeth (Ohshima et al 2000(Ohshima et al , 2002. Recently Hsp25 has been reported that related to cell diVerentiation during tooth development, especially amelogenesis (Nakasone et al 2006;Ohshima et al 2002;Otsuka et al 2001).…”

Section: Introductionmentioning

confidence: 98%

MAPK mediates Hsp25 signaling in incisor development

Lee

Cai

Kwak

et al. 2009

Histochem Cell Biol

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Rodent incisors are continuously growing teeth that include all stages of amelogenesis. Understanding amelogenesis requires investigations of the genes and their gene products control the ameloblast phenotype. One of the mechanisms related to tooth differentiation is mitogen-activated protein kinase (MAPK) signaling. The extracellular-signal regulated kinase (ERK)/mitogen-activated protein kinase kinase (MEK) cascade is associated with mechanisms that control the cell cycle and cell survival. However, the roles of cascades in incisor development remain to be determined. In this study, we investigated incisor development and growth in the mouse based on MAPK signaling. Moreover, heat-shock protein (Hsp)-25 is well known to be a useful marker of odontoblast differentiation. We used anisomycin (a protein-synthesis inhibitor that activates MAPKs) and U0126 (a MAPK inhibitor that blocks ERK1/2 phosphorylation) to examine the role of MAPKs in Hsp25 signaling in the development of the mouse incisor. We performed immunohistochemistry and in vitro culture using incisor tooth germ, and found that phospho-ERK (pERK), pMEK, and Hsp25 localized in developing incisor ameloblasts and anisomycin failed to produce incisor development. In addition, Western blotting results showed that anisomycin stimulated the phosphorylation of ERK, MEK, and Hsp25, and that some of these proteins were blocked by the U0126. These findings suggest that MAPK signals play important roles in incisor formation, differentiation, and development by mediating Hsp25 signaling.

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Different expression of 25-kDa heat-shock protein (Hsp25) in Meckel's cartilage compared with other cartilages in the mouse

Cited by 18 publications

References 43 publications

Expression of Heat Shock Protein 27 with the Transition from Proliferation to Differentiation of Acinar Precursor Cell in Regenerating Submandibular Gland of Rats

Expression of Heat Shock Protein 27 with the Transition from Proliferation to Differentiation of Acinar Precursor Cell in Regenerating Submandibular Gland of Rats

Immunolocalization of receptor activator of nuclear factor‐κB ligand (RANKL) and osteoprotegerin (OPG) in Meckel's cartilage compared with developing endochondral bones in mice

MAPK mediates Hsp25 signaling in incisor development

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