Different effects of lipoteichoic acid from C. butyricum and S. aureus on inflammatory responses of HT-29 cells

Wang, Jinbo; Qi, Lili; Wu, Zhongqi; Mei, Lehe; Wang, Hengzheng

doi:10.1016/j.ijbiomac.2016.03.010

Cited by 10 publications

(5 citation statements)

References 29 publications

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“…The 1 H NMR spectra of LTA samples were complicated due to the plentiful peak overlaps in these samples. Combined with previous studies [ 6 , 22 ], it was postulated that the three Lactobacillus strains had significant differences in the length and saturation of α-D-N-acetylglucosamine, alanine, and acyl fatty acid chains.…”

Section: Resultsmentioning

confidence: 81%

“…Lactobacillus LTAs were extracted and purified as previously described [ 21 , 22 ]. Briefly, 10 g of the bacteria pellets was dissolved in 50 mL of sodium citrate buffer (0.1 M, pH 4.7).…”

Section: Methodsmentioning

confidence: 99%

“…The structure of LTA was identified by Fourier transform infrared spectroscopy (FT-IR) analyses and nuclear magnetic resonance (NMR) spectrometry according to previous methods [ 22 , 23 ]. After the standard LTA of S. aureus and those from Lactobacillus were fully mixed with KBr, ground, and pressed.…”

Section: Methodsmentioning

confidence: 99%

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Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains

Lü

Guo

et al. 2022

Foods

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Lactobacillus are normal inhabitants of the gastrointestinal tract and confer a variety of health effects. Lipoteichoic acid (LTA), an amphiphilic substance located in the cell membrane, is a key molecule in probiotic–host crosstalk. Through the characterization of structural characteristics of LTA molecules derived from Lactobacillus plantarum A3, Lactobacillus reuteri DMSZ 8533, and Lactobacillus acidophilus CICC 6074, there exists some heterogeneity in LTA molecules, which perhaps contributes to the distinguishable adhesion properties of Lactobacillus strains based on fluorescence microscopy observations. In LPS-induced RAW 264.7 cells, LTAs derived from three Lactobacillus strains obviously alleviated inflammatory responses as evidenced by the altered inflammatory cytokine levels of TNF-α, IL-6, and IL-10. Western blotting demonstrated that L. reuteri LTA blocked LPS-triggered expression of the MAPK and NF-κB pathways. The findings further validated that LTA is an important effector molecule and deserves further consideration as an alternative therapeutic for ulcerative colitis treatment.

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Section: Resultsmentioning

confidence: 81%

“…Lactobacillus LTAs were extracted and purified as previously described [ 21 , 22 ]. Briefly, 10 g of the bacteria pellets was dissolved in 50 mL of sodium citrate buffer (0.1 M, pH 4.7).…”

Section: Methodsmentioning

confidence: 99%

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Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains

Lü

Guo

et al. 2022

Foods

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“…LTA from S. aureus stimulates host cells to produce inflammatory cytokines such as IL-8 and tumor necrosis factor-α by the activation of NF-κB [ 25 , 26 ]. LTAs from pathogenic bacteria, including S. aureus , S. epidermidis , and Streptococcus pneumonia could activate macrophages.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of a Novel Lactobacillus brevis Strain with Probiotic Characteristics against Staphylococcus aureus Lipoteichoic Acid-Induced Intestinal Inflammatory Response

Kim

Hyun

Lee

et al. 2022

J. Microbiol. Biotechnol.

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Probiotics can effectively modulate host immune responses and prevent gastrointestinal diseases.The objective of this study was to investigate the probiotic characteristics of Lactobacillus brevis KU15152 isolated from kimchi and its protective potential against intestinal inflammation induced by Staphylococcus aureus lipoteichoic acid (aLTA). L. brevis KU15152 exhibited a high survival rate in artificial gastric and bile environments. Additionally, the adhesion capability of the strain to HT-29 cells was higher than that of L. rhamnosus GG. L. brevis KU15152 did not produce harmful enzymes, such as β-glucuronidase, indicating that it could be used as a potential probiotic. The antiinflammatory potential of L. brevis KU15152 was determined in HT-29 cells. Treatment with L. brevis KU15152 suppressed the production of interleukin-8 without inducing significant cytotoxicity. The downregulatory effects of L. brevis KU15152 were involved in the suppression of nuclear factorkappa B activation mediated by the extracellular signal-regulated kinase and Akt signaling pathways. Collectively, these data suggest that L. brevis KU15152 can be used in developing therapeutic and prophylactic products to manage and treat aLTA-induced intestinal damage.

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“…It has been reported that the Lactobacillus strains not inducing IL-8 production by gastrointestinal epithelial cells could alleviate enteritis (Candela et al, 2008;Panpetch et al, 2016). The chemical difference in cell wall components between probiotic bacteria and pathogenic bacteria may account for the different pro-inflammatory cytokine expression of host cells (Wang et al, 2016).…”

Section: Effect Of Lactobacillus Strains On E Coli-induced Il-8 Production By Ht-29 Cellsmentioning

confidence: 99%

Screening probiotics from Lactobacillus strains according to their abilities to inhibit pathogen adhesion and induction of pro-inflammatory cytokine IL-8

Tuo

Xing

Song

et al. 2018

Journal of Dairy Science

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Probiotics can be screened according to their abilities to inhibit pathogen adhesion and inhibit the production of pro-inflammatory cytokines. Eleven Lactobacillus strains isolated from traditional fermented dairy foods in Xinjiang, China, were studied for their potential to inhibit adhesion of Escherichia coli to intestinal epithelial cells and to inhibit E. coli-induced production of interleukin (IL)-8 by intestinal epithelial cells. The results showed that the 11 strains could inhibit adhesion of E. coli to Caco-2 cell monolayers and inhibit the induction of IL-8 production by E. coli in HT-29 cells. The inhibiting activities of the Lactobacillus strains against E. coli adhesion and IL-8 induction were strain-specific and not positively correlated, whereas the excluding activity of the strains against E. coli adhesion and their coaggregation with E. coli were positively correlated. The effector molecules of the strains with probiotic potential should be identified to explain the mechanism behind these observations.

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Different effects of lipoteichoic acid from C. butyricum and S. aureus on inflammatory responses of HT-29 cells

Cited by 10 publications

References 29 publications

Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains

Structure and Anti-Inflammation Potential of Lipoteichoic Acids Isolated from Lactobacillus Strains

Protective Effects of a Novel Lactobacillus brevis Strain with Probiotic Characteristics against Staphylococcus aureus Lipoteichoic Acid-Induced Intestinal Inflammatory Response

Screening probiotics from Lactobacillus strains according to their abilities to inhibit pathogen adhesion and induction of pro-inflammatory cytokine IL-8

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