Different Diets Change the Expression of Bovine Serum Extracellular Vesicle-miRNAs

Quan, Suyu; Nan, Xuemei; Wang, Kun; Jiang, Linshu; Yao, Junhu; Xiong, Bin

doi:10.3390/ani9121137

Cited by 10 publications

(6 citation statements)

References 32 publications

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“…The process of bioinformatics analysis was conducted as we did before [17]. Brifely, the low-quality reads, 5 primer contaminants and poly (A) were removed from raw reads by Cutadapt (version 1.14) (https://cutadapt.readthedocs.io/en/stable/) and the sequences between 15 and 41 nt were retained.…”

Section: Bioinformatics Analysismentioning

confidence: 99%

Characterization of Sheep Milk Extracellular Vesicle-miRNA by Sequencing and Comparison with Cow Milk

Quan

Nan

Wang

et al. 2020

Animals

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Milk can mediate maternal-neonatal signal transmission by the bioactive component extracellular vesicles (EVs), which select specific types of miRNA to encapsulate. The miRNA profiling of sheep milk EVs was characterized by sequencing and compared with that of cow milk. Nanoparticle tracking analysis revealed that the concentration of sheep milk EVs was 1.3 ± 0.09 × 1012 particles/mL and the diameter was peaked at 131.2 ± 0.84 nm. Sheep milk EVs contained various small RNAs, including tRNA, Cis-regulatory element, rRNA, snRNA, other Rfam RNA, and miRNA, which held about 36% of all the small RNAs. In total, 84 types of miRNA were annotated with Ovis aries by miRBase (version 22.0) in sheep milk EVs, with 75 shared types of miRNAs in all samples. The miR-26a, miR-191, let-7f, let-7b and miR-10b were highly expressed both in cow and sheep milk EVs, and 14 sheep milk EV-miRNAs in the top 20, occupying 98% of the total expression, were immune-related. Although pathway analysis showed different potential functions of cow and sheep milk EV-miRNAs, there were still some shared points: lipid metabolism (phospholipase D, glycerophospholipid and glycosylphosphatidylinositol), calcium metabolism, and nerve conduction (axon guidance and synapse). This study provides reference for the bioactive components in the milk of different species.

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Section: Bioinformatics Analysismentioning

confidence: 99%

Characterization of Sheep Milk Extracellular Vesicle-miRNA by Sequencing and Comparison with Cow Milk

Quan

Nan

Wang

et al. 2020

Animals

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“…We previously demonstrated that the partial replacement of forage with NFFS in dairy cow diets could decrease the ratio of ruminal acetate to propionate, which might change the whole metabolism through energy supply, including milk synthesis [14]. Furthermore, we found that the NFFS diets influenced the expression of circulatory and milk EV-miRNA, with increased particle concentration of milk EVs [15,16]. Hence, this study hypothesizes that the replacement of forage with NFFS could change the cow milk EV-protein composition.…”

Section: Introductionmentioning

confidence: 90%

Different Diets Change Milk Extracellular Vesicle-Protein Profile in Lactating Cows

Quan

Wang

et al. 2022

Agriculture

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We previously demonstrated that the partial replacement of forage with non-forage fiber sources (NFFS) in dairy cow diets could decrease the ruminal ratio of acetate to propionate, leading to changes in circulatory and milk extracellular vesicle (EV)-miRNAs expression. This study further explored the effects of the NFFS diets on milk EV proteins, which were proposed as food bio-active ingredients in recent research. We replaced 8.97% alfalfa hay and 2.51% corn silage with 5.72% whole cotton seed and 4.73% soybean hull in the cow diet, reducing the forage neutral detergent fiber from 20.92% to 15.67%. In total 488 proteins were identified by proteome, and 65 proteins were differentially expressed in response to the NFFS diets, the functions of which were mainly enriched in immune-related pathways, including complement and coagulation cascades, phagosome, Staphylococcus aureus infection, and chemokine signaling pathway. Moreover, 57 milk EV-proteins, mainly attributed to enzymes, cytoskeletal proteins, and transport proteins, were in the top 100 most identified EV-proteins in different species and body fluids, which might be related to the biogenesis, structure, and traffic of all vesicles. The results showed that NFFS diets could influence cow milk EV-protein composition, implying that we could take effective nutritional strategies to promote the synthesis of milk functional ingredients. Combined with all our studies, NFFS diets were recommended to improve the rumen fermentation model and enrich the milk EV proteins of dairy cows.

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“…In a recent study, it was discovered that different diets could affect miRNA expression of bovine serum, and their potential influence on immunity was discovered as well. The findings suggested that one could use effective dietary strategies to interfere with the physiological state of animals [ 99 ].…”

Section: Development Of Mirnas As Biomarkers Of Diet Nutritional Stat...mentioning

confidence: 99%

MicroRNAs in Ruminants and Their Potential Role in Nutrition and Physiology

Ojo

Kreuzer-Redmer

2023

Veterinary Sciences

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The knowledge of how diet choices, dietary supplements, and feed intake influence molecular mechanisms in ruminant nutrition and physiology to maintain ruminant health, is essential to attain. In the present review, we focus on the role of microRNAs in ruminant health and disease; additionally, we discuss the potential of circulating microRNAs as biomarkers of disease in ruminants and the state of technology for their detection, also considering the major difficulties in the transition of biomarker development from bench to clinical practice. MicroRNAs are an inexhaustible class of endogenous non-protein coding small RNAs of 18 to 25 nucleotides that target either the 3′ untranslated (UTR) or coding region of genes, ensuring a tight post-transcriptionally controlled regulation of gene expression. The development of new “omics” technologies facilitated a fresh perspective on the nutrition–to–gene relationship, incorporating more extensive data from molecular genetics, animal nutrition, and veterinary sciences. MicroRNAs might serve as important regulators of metabolic processes and may present the inter-phase between nutrition and gene regulation, controlled by the diet. The development of biomarkers holds the potential to revolutionize veterinary practice through faster disease detection, more accurate ruminant health monitoring, enhanced welfare, and increased productivity. Finally, we summarize the latest findings on how microRNAs function as biomarkers, how technological paradigms are reshaping this field of research, and how platforms are being used to identify novel biomarkers. Numerous studies have demonstrated a connection between circulating microRNAs and ruminant diseases such as mastitis, tuberculosis, foot-and-mouth disease, fasciolosis, and metabolic disorders. Therefore, the identification and analysis of a small number of microRNAs can provide crucial information about the stage of a disease, etiology, and prognosis.

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Different Diets Change the Expression of Bovine Serum Extracellular Vesicle-miRNAs

Cited by 10 publications

References 32 publications

Characterization of Sheep Milk Extracellular Vesicle-miRNA by Sequencing and Comparison with Cow Milk

Characterization of Sheep Milk Extracellular Vesicle-miRNA by Sequencing and Comparison with Cow Milk

Different Diets Change Milk Extracellular Vesicle-Protein Profile in Lactating Cows

MicroRNAs in Ruminants and Their Potential Role in Nutrition and Physiology

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