Different binding properties of two general-odorant binding proteins in Athetis lepigone with sex pheromones, host plant volatiles and insecticides

Zhang, Xiaoqing; Yan, Qi; Li, Lulu; Xu, Junwei; Mang, Dingze; Wang, Xueliang; Hoh, Hong-Huat; Jia, Yali; Ju, Qian; Ma, Yu; Liang, Meng; Zhang, Yunying; Zhu, Xiu-Yun; Zhang, Fan; Dong, Shuang-Lin; Zhang, Yanan; Zhang, Long-Wa

doi:10.1016/j.pestbp.2020.01.012

Cited by 50 publications

(88 citation statements)

References 63 publications

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“…The antennal olfactory system of A. lepigone house specific neurons that respond electrophysiologically to sex pheromones and plant volatiles from hosts and food sources (Zhang et al, 2016, 2019, 2020). The olfactory function is mainly associated with sensilla trichodea (Keil, 1989; Liu et al, 2018), basiconica (Bawin et al, 2017; Liu et al, 2018), and auricillica (Anderson et al, 2000; Ansebo et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Sensilla of the antenna and proboscis of Athetis lepigone (Möschler) (Lepidoptera: Noctuidae)

Zhang

Wang

et al. 2021

Journal of Morphology

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Sensory structures on the antennae and mouthparts of insects are associated with various activities, such as host location, feeding, attracting a mate, and identifying a suitable oviposition site. Athetis lepigone (Möschler) is an important polyphagous Eurasian pest with more than 30 species of host plants. The larvae target bud leaves, prop roots, and tender stems of many agricultural crops, but the feeding habits of the adults remain poorly known. Aiming to understand the feeding behavior of the species, we investigated the fine morphology of its antennae and proboscis using scanning electron microscopy. The antennae of both sexes are filiform, and bear eight types of sensilla: Böhm's bristles, sensilla squamiformia, trichodea, chaetica, basiconica, coeloconica, styloconica, and auricillica. Sensilla trichodea are the most abundant among these sensillum types. The proboscis consists of two elongated, interlocked maxillary galeae that enclose the food canal by dorsal and ventral legulae. The external galeal surface is covered with numerous triangular microtrichia on Zone 1 and abundant blunt microbumps on Zone 2. The surface of the food canal bears closely connected and smooth semicircular ridges, gradually tapering toward the proboscis tip. Three types of sensilla are noticeable on the proboscis: sensilla trichodea, basiconica, and styloconica. We briefly discuss the putative functional significance of the antennal and proboscis sensilla and, based on the specific structural modifications of the proboscis, predict a flower‐visiting habit for A. lepigone.

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Section: Discussionmentioning

confidence: 99%

Sensilla of the antenna and proboscis of Athetis lepigone (Möschler) (Lepidoptera: Noctuidae)

Zhang

Wang

et al. 2021

Journal of Morphology

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“…In Culex pipiens , expression of OBPjj7a and OBP28 in the 4th larval instar correlated positively with deltamethrin resistance ( Li et al, 2016 ; Liu et al, 2017 ). Furthermore, in vitro binding tests found that OBPs have high binding affinity with insecticides ( Li H. et al, 2015 ; Li et al, 2017 ; Zhang et al, 2020 ). Here, we investigated the OBP gene TcOBPC11 in T. castaneum and revealed that expression of this gene was significantly induced by EO-AV.…”

Section: Discussionmentioning

confidence: 99%

“…Although OBPs are highly divergent between insects, they contain conserved cysteine residues that are bonded by interlocked disulfide bridges ( Leal et al, 1999 ; Scaloni et al, 1999 ). OBP genes are expressed at higher levels in adult antennae than in other apparatus, suggesting their participation in odorant identification by adult insects ( Chang et al, 2017 ; Huang et al, 2018 ; Zhang et al, 2020 ). The functions of OBPs have been verified by means of in vitro binding experiments in a diverse range of insects such as D. melanogaster ( Larter et al, 2016 ), B. mori ( Zhou et al, 2009 ), Aedes aegypti ( Kim et al, 2017 ), and Periplaneta americana ( He et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Odorant-Binding Proteins Contribute to the Defense of the Red Flour Beetle, Tribolium castaneum, Against Essential Oil of Artemisia vulgaris

et al. 2020

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The function of odorant-binding proteins (OBPs) in insect chemodetection has been extensively studied. However, the role of OBPs in the defense of insects against exogenous toxic substances remains elusive. The red flour beetle, Tribolium castaneum , a major pest of stored grains, causes serious economic losses for the agricultural grain and food processing industries. Here, biochemical analysis showed that essential oil (EO) from Artemisia vulgaris , a traditional Chinese medicine, has a strong contact killing effect against larvae of the red flour beetle. Furthermore, one OBP gene, TcOBPC11 , was significantly induced after exposure to EO. RNA interference (RNAi) against TcOBPC11 led to higher mortality compared with the controls after EO treatment, suggesting that this OBP gene is associated with defense of the beetle against EO and leads to a decrease in sensitivity to the EO. Tissue expression profiling showed that expression of TcOBPC11 was higher in the fat body, Malpighian tubule, and hemolymph than in other larval tissues, and was mainly expressed in epidermis, fat body, and antennae from the early adult. The developmental expression profile revealed that expression of TcOBPC11 was higher in late larval stages and adult stages than in other developmental stages. These data indicate that TcOBPC11 may be involved in sequestration of exogenous toxicants in the larvae of T. castaneum . Our results provide a theoretical basis for the degradation mechanism of exogenous toxicants and identify potential novel targets for controlling the beetle.

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“…Another potential function of these genes is as transporters that bind xenobiotic compounds, especially insecticides. In Apis cerana Fabricius (Hymenoptera: Apidae), Athetis lepigone (Möschler) (Lepidoptera: Noctuidae), Ectropis obliqua Prout (Lepidoptera: Geometridae), and S. litura , OBPs can interact with various insecticides and may contribute to defense against these harmful xenobiotic compounds ( Li et al 2015 , 2017 ; Zhang et al 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Identification and Expression Profiles of 14 Odorant-Binding Protein Genes From Pieris rapae (Lepidoptera: Pieridae)

Jiang

et al. 2020

Journal of Insect Science

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The small white butterfly, Pieris rapae (L.), is an important insect pest of Brassica crops. This species utilize olfactory cues to find their hosts and mates. However, the molecular mechanism underlying the olfactory perception in this species remains unclear. Here, we identified 14 odorant-binding proteins (OBP) genes—essential for insect olfaction—in P. rapae by exploring a previously published transcriptome dataset. Proteins encoded by all of these genes contain N-terminal signal peptides and six positionally conserved cysteine residues, which are characteristic of insect OBPs. These OBPs displayed high amino acid identity with their respective orthologs in other lepidopterans, and several conserved motifs were identified within these OBPs. Phylogenetic analysis showed that these OBPs were well segregated from each other and clustered into different branches. PrapOBP1 and PrapOBP2 were clustered into the ‘general odorant-binding protein’ clade, and PrapOBP3 and PrapOBP4 fall into the ‘pheromone-binding protein’ clade. The 14 OBP genes were located on seven genomic scaffolds. Of these, PrapOBP1, 2, 3, and 4 were located on scaffold332, whereas PrapOBP5, 6, 7, 8, and 9 were located on scaffold116. Ten of the 14 genes had antenna-biased expression. Of these, PrapOBP1, 2, 4, and 13 were enriched in male antennae, whereas PrapOBP7 and PrapOBP10 were female-biased. Our findings suggest that these OBPs may be involved in olfactory communication. To the best of our knowledge, this is the first report on the identification and characterization of OBPs in P. rapae, and our findings provide a solid foundation for studying the functions of these genes.

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Different binding properties of two general-odorant binding proteins in Athetis lepigone with sex pheromones, host plant volatiles and insecticides

Cited by 50 publications

References 63 publications

Sensilla of the antenna and proboscis of Athetis lepigone (Möschler) (Lepidoptera: Noctuidae)

Sensilla of the antenna and proboscis of Athetis lepigone (Möschler) (Lepidoptera: Noctuidae)

Odorant-Binding Proteins Contribute to the Defense of the Red Flour Beetle, Tribolium castaneum, Against Essential Oil of Artemisia vulgaris

Identification and Expression Profiles of 14 Odorant-Binding Protein Genes From Pieris rapae (Lepidoptera: Pieridae)

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