Different aspects of platelet evaluation in dengue: Measurement of circulating mediators, ability to interact with the virus, the degree of activation and quantification of intraplatelet protein content

Azamor, Tamiris; Batista, Débora de Oliveira; Carvalho, Amanda Torrentes de; Faria, Nieli Rodrigues da Costa; Jácome, Fernanda Cunha; Barth, Ortrud Monika; Nogueira, Rita Maria Ribeiro; Neves, Patrícia Cristina da Costa; Matos, Denise Cristina de Souza; Azeredo, Elzinandes Leal de; Damasco, Paulo Vieira; Cunha, Rivaldo Venâncio da; de-Oliveira-Pinto, Luzia Maria

doi:10.1016/j.virusres.2018.09.013

Cited by 6 publications

(9 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TXB 2 is the stable product of the non-enzymatic hydration of thromboxane A2 (TXA 2 ), thus the production of TXA 2 in vivo is typically monitored by measurement of TXB 2 ( 27 ). Therefore, as previous studies ( 28 – 31 ), we chose the R&D’s TXB 2 competitive ELISA kit (R&D Systems, United States) to detect TXB 2 for reflecting the production of TXA 2 . The SPARK ® multimode microplate reader (Tecan, Switzerland) was used to record ODs at 450 nm.…”

Section: Methodsmentioning

confidence: 99%

Four-Week Supplementation of Water-Soluble Tomato Extract Attenuates Platelet Function in Chinese Healthy Middle-Aged and Older Individuals: A Randomized, Double-Blinded, and Crossover Clinical Trial

Tian

Fan²,

Li³

et al. 2022

Front. Nutr.

View full text Add to dashboard Cite

Background and AimsPlatelets are linked to atherosclerotic development and pathological thrombosis. Single dose of water-soluble tomato extract (WTE) which is a natural extraction can exert anti-platelet effects after 3 or 7 h in British healthy people. However, the effects of WTE supplementation on platelet function in Chinese healthy middle-aged and older individuals have not been studied, and the effects or safety of 4-week WTE supplementation also remain unclear. The present study aims to determine the effects of WTE on platelet function, and explore the safety of 4-week WTE supplementation in Chinese healthy middle-aged and older individuals.MethodsA randomized, double-blinded, and crossover clinical trial was conducted. Firstly, 105 individuals were randomly divided into two groups that received WTE (150 mg/day) or placebo for 4 weeks. Then, after a washout period of 2 weeks, two groups exchanged groups and continued for another 4-week intervention. Platelet aggregation, P-selectin, activated GPIIbIIIa, plasma platelet factor 4 (PF4), β-thromboglobulin (β-TG), and thromboxane B2 (TXB2) were tested at baseline, 4, 6, and 10 weeks.ResultsCompared with the placebo group, 150 mg/day WTE supplement for 4 weeks significantly reduced ADP-induced or collagen-induced platelet aggregation (−10.8 ± 1.8 or −3.9 ± 1.5%, P < 0.05), ADP-induced or collagen-induced platelet P-selectin expression (−6.9 ± 1.5 or −6.6 ± 1.3%, P < 0.05), ADP-induced or collagen-induced activated GPIIbIIIa (−6.2 ± 2.0 or −3.8 ± 2.0%, P < 0.05). Besides, 4-week intervention of 150 mg WTE per day also resulted in significant reductions in plasma PF4 (−120.6 ± 33.2 ng/mL, P < 0.05) and β-TG (−129.7 ± 27.5 ng/mL, P < 0.05) and TXB2 (−42.0 ± 4.0 ng/mL, P < 0.05), while had no effects on coagulation function and liver or renal function. Interestingly, 2-week washout period is enough to reverse the inhibitory effect of 4-week WTE supplementation on platelet function.ConclusionWTE supplementation for 4 weeks could moderately reduce platelet activation, aggregation, and granule secretion in Chinese healthy middle-aged and older individuals, and these effects are safe. After 2-week washout period, the inhibitory effect of 4-week WTE on platelet function can be eliminated.Clinical Trial Registration[http://www.chictr.org.cn/], identifier [ChiCTR-POR-17012927].

show abstract

Section: Methodsmentioning

confidence: 99%

Four-Week Supplementation of Water-Soluble Tomato Extract Attenuates Platelet Function in Chinese Healthy Middle-Aged and Older Individuals: A Randomized, Double-Blinded, and Crossover Clinical Trial

Tian

Fan²,

Li³

et al. 2022

Front. Nutr.

View full text Add to dashboard Cite

show abstract

“…With regard to immunological responses, platelets present multiple immune receptors, such as immunoglobulin or complement receptors and Toll-like receptors (TLRs) [85, 94], and the capacity of NRLP-3 inflammasome activation and production of cytokines IL-1β and TGF-β [95][96][97]. These functions are achieved through direct interaction with endothelial cells, as well as monocytes, lymphocytes (plateletleukocyte aggregates (PLA)) and neutrophils (platelet-neutrophil aggregates (PNA)) in a process regulated by Pselectin [93,98,99].…”

Section: Hemostatic Disorders In Covid-19: a Consequence Of Immune Events?mentioning

confidence: 99%

Cellular and Molecular Immunology Approaches for the Development of Immunotherapies against the New Coronavirus (SARS-CoV-2): Challenges to Near-Future Breakthroughs

Melgaço

Cunha

Azamor

et al. 2020

Journal of Immunology Research

View full text Add to dashboard Cite

The severe acute respiratory syndrome caused by the new coronavirus (SARS-CoV-2), termed COVID-19, has been highlighted as the most important infectious disease of our time, without a vaccine and treatment available until this moment, with a big impact on health systems worldwide, and with high mortality rates associated with respiratory viral disease. The medical and scientific communities have also been confronted by an urgent need to better understand the mechanism of host-virus interaction aimed at developing therapies and vaccines. Since this viral disease can trigger a strong innate immune response, causing severe damage to the pulmonary tract, immunotherapies have also been explored as a means to verify the immunomodulatory effect and improve clinical outcomes, whilst the comprehensive COVID-19 immunology still remains under investigation. In this review, both cellular and molecular immunopathology as well as hemostatic disorders induced by SARS-CoV-2 are summarized. The immunotherapeutic approaches based on the most recent clinical and nonclinical studies, emphasizing their effects for the treatment of COVID-19, are also addressed. The information presented elucidates helpful insights aiming at filling the knowledge gaps around promising immunotherapies that attempt to control the dysfunction of host factors during the course of this infectious viral disease.

show abstract

“…Seven studies reported lower levels of VEGF in dengue patients than in healthy controls [ 34 , 35 , 36 , 37 , 38 , 39 , 40 ], including dengue with warning signs [ 35 , 38 ] and severe dengue [ 34 , 36 , 39 , 40 ]. No difference in results was found between serum and plasma samples [ 39 ].…”

Section: Resultsmentioning

confidence: 99%

The Role of Growth Factors in the Pathogenesis of Dengue: A Scoping Review

2022

View full text Add to dashboard Cite

Growth factors (GFs) have a role in tissue repair and in the modulation of the expression of inflammatory cells in damage caused by pathogens. This study aims to systematize the evidence on the role of GFs in the pathogenesis of dengue. This scoping review considered all published peer-reviewed studies in the MEDLINE and Embase databases. Ultimately, 58 studies that analyzed GFs in dengue patients, published between 1998 and 2021, were included. DENV-2 infection and secondary infection were more frequent in the patients studied. ELISA and multiplex immunoassay (Luminex) were the most used measurement techniques. Increased levels of vascular endothelial growth factor, granulocyte–macrophage colony-stimulating factor, granulocyte colony-stimulating factor, transforming growth factor beta, and hepatocyte growth factor as well as reduced levels of platelet-derived growth factor and epidermal growth factor were observed in severe dengue in most studies. Vascular endothelial growth factor and hepatocyte growth factor were identified as biomarkers of severity. In addition, there is evidence that the dengue virus can use the growth factor pathway to facilitate its entry into the cell and promote its viral replication. The use of tyrosine kinase inhibitors is an alternative treatment for dengue that is being studied.

show abstract

Different aspects of platelet evaluation in dengue: Measurement of circulating mediators, ability to interact with the virus, the degree of activation and quantification of intraplatelet protein content

Cited by 6 publications

References 57 publications

Four-Week Supplementation of Water-Soluble Tomato Extract Attenuates Platelet Function in Chinese Healthy Middle-Aged and Older Individuals: A Randomized, Double-Blinded, and Crossover Clinical Trial

Four-Week Supplementation of Water-Soluble Tomato Extract Attenuates Platelet Function in Chinese Healthy Middle-Aged and Older Individuals: A Randomized, Double-Blinded, and Crossover Clinical Trial

Cellular and Molecular Immunology Approaches for the Development of Immunotherapies against the New Coronavirus (SARS-CoV-2): Challenges to Near-Future Breakthroughs

The Role of Growth Factors in the Pathogenesis of Dengue: A Scoping Review

Contact Info

Product

Resources

About