Differences in the protection elicited by a recombinant Teladorsagia circumcincta vaccine in weaned lambs of two Canarian sheep breeds

Pérez-Hernández, Tara; Corripio-Miyar, Yolanda; Hernández, Julia N.; Machín, Cynthia; Paz-Sánchez, Yania; Hayward, Adam D.; Wright, Harry W.; Price, Daniel D.; Matthews, Jacqueline B.; McNeilly, Tom N.; Nisbet, Alasdair J.; González, J.

doi:10.1016/j.vetpar.2022.109722

Cited by 7 publications

(8 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The local response to T. circumcincta, observed here, has also been observed in sheep immunised with a recombinant prototype vaccine [41] and in sheep [42] and goats naturally resistant to the parasite [25]. In all these cases, specific IgA antibodies have been shown to be associated with defensive mechanisms against the parasite (mainly related to a reduction in fertility), supporting the idea of the relevance of this immunoglobulin isotype in immunoprotection against T. circumcincta.…”

Section: Discussionsupporting

confidence: 82%

“…Its function is directed towards macrophage activation and the induction of CD8 lymphocyte proliferation [46,47]. The increased IL-2 transcription in lymph nodes, which apparently seems to contrast with the results observed at the gastric mucosal level, could be related to the development of mixed Th1/Th2 observed by some authors in gastric nematode infections in ruminants after previous sensitization [41,48], or in cattle and sheep vaccinated against O. ostertagi and T. circumcincta, respectively [49,50]. Th17 and/or Treg-mediated responses may also play a role in generating protection against challenge [51]; however, both types of response did not exhibit a significant expression in the present study.…”

Section: Discussionmentioning

confidence: 92%

See 1 more Smart Citation

Analysis of Protection and Immune Response against Teladorsagia circumcincta in Goats Immunised with Thiol-Binding Proteins from Adult Worms

Ortega,

Quesada,

Ruiz

et al. 2024

Vaccines

View full text Add to dashboard Cite

In view of the increasing occurrence of anthelmintic-resistant strains of gastrointestinal nematodes in ruminants, various alternative control strategies have been investigated, such as those based on the induction of protective immune responses by immunisation with parasite antigens. In this study, the protective activity of somatic antigens from adult worms of Teladorsagia circumcincta purified by affinity chromatography on thiol-sepharose was analysed in goats. After challenge, the enriched products induced a slight reduction in the cumulative faecal egg counts (21%) and in the number of worms (23.3%), with a greater effect on female worms, which also showed a reduction in parameters related to their fertility. These parasitological findings were associated with a Th2 immune response, with a prominent local humoral response and an eosinophilic infiltrate in the gastric mucosa (negatively associated with the fertility of female worms and the number of worms, respectively), as well as an infiltration of MCHII+, CD4+, IgG+ and IgA+ cells. However, several analyses showed an increase in CD8+ cells in the mucosa, as well as IL-2 expression in the gastric lymph nodes, which may have been associated with inhibition of protective responses or with the development of mixed Th1/Th2 responses, a finding that should be analysed in future studies.

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 92%

Analysis of Protection and Immune Response against Teladorsagia circumcincta in Goats Immunised with Thiol-Binding Proteins from Adult Worms

Ortega,

Quesada,

Ruiz

et al. 2024

Vaccines

View full text Add to dashboard Cite

show abstract

“…The immune response against GIN involves the production of IgA, and its increase has been related to more resistant sheep under experimental conditions, especially against T. circumcincta and H. contortus . Indeed, in experimentally infected sheep, increasedlevels of IgA in adult Churra sheep and lambs belonging to the Scottish Blackface and Canarian Hair breeds have been associated with shorter female nematodes and, consequently, with a lower number of female worm eggs in utero [ 25 , 43 , 44 ]. Most of the IgA present in saliva is produced by B-lymphocytes that migrate through the blood from the lymphoid tissue associated with gastrointestinal mucosa; however, the relationship between IgA in saliva and plasma remains to be determined [ 45 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

Secretory IgA as Biomarker for Gastrointestinal Nematodes Natural Infection in Different Breed Sheep

Agüero

Valderas-García

Palacio

et al. 2023

Animals

View full text Add to dashboard Cite

Specific IgA antibody has been shown to play an important role in resistance to gastrointestinal nematode (GIN) infections in sheep, particularly in Teladorsagia circumcincta parasitosis. In some breeds, negative associations have been shown between IgA levels and worm burden in experimentally infected sheep. In the present study, we have studied the relationship between IgA levels in naturally infected sheep (582 ewes in total; 193 younger than one year old and 389 older than one year old) and fecal egg count (FEC) in the Assaf, Castellana, and Churra breeds. ELISA assays were performed to measure IgA levels against the somatic antigen of T. circumcincta third larval stage (L3) and a 203-amino-acid fragment of the protein disulfide isomerase from the same GIN species. A multilevel random intercept model was developed to predict the infection risk according to age or breed. Spearman’s correlation rank was used for statistical analysis. The prediction model showed that breed was not an influential factor in this study, although the Assaf breed could be considered slightly more susceptible than the others. In addition, age affected the infection risk, with the young ewes more susceptible to infection than the adult groups, except for the Castellana breed, whose risk of infection was similar at all ages. The most significant positive association was found between FEC and IgA measured in the nasal secretions of young ewes using both antigens (Rho = 0.5; p = 0.00); the correlation of FEC with IgA in serum was moderately significant (Rho = 0.306; p = 0.00). Comparing both antigens, the protein disulfide isomerase antigen was less reactive than the somatic antigen from L3. In conclusion, under natural conditions, specific IgA against GIN was positively associated with FEC in sheep, with nasal secretions from young animals being the sample where this association is stronger, which, therefore, could be used as a marker of infection in further studies.

show abstract

“…The CHB and the CS breeds have been used previously as models in several studies because of differences in their resistance to H. contortus and T. circumcincta in lambs older than six months of age [16,23]. Moreover, a recent study demonstrated that three -month-old CHB lambs responded to vaccination with recombinant T. circumcincta proteins, with reductions in parasitological variables and involvement of well-characterised anti-GIN immune mechanisms described in adult animals, whereas such differences were not observed in three-month-old CS lambs [29]. In contrast, here, significant differences in susceptibility to T. circumcincta in unvaccinated lambs of these two breeds at three months of age were not identified.…”

Section: Discussionmentioning

confidence: 99%

“…The experimental design was previously described in detail in the study of Pérez-Hernández et al [29]. Briefly, three-month-old CHB (n = 12) and CS (n = 12) male lambs were purchased from several farms in Gran Canaria and were dewormed on arrival with fenbendazole.…”

Section: Animals and Parasitologymentioning

confidence: 99%

Variability in the Response against Teladorsagia circumcincta in Lambs of Two Canarian Sheep Breeds

Pérez-Hernández

Hernández

Machín

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

The increasing resistance to anthelmintics has necessitated the exploration of alternative control strategies of gastrointestinal nematode (GIN) infections. A sustainable option is genetic selection based on differences in susceptibility to GIN infection between and within breeds of sheep. Here, three-month-old Canaria Hair breed (GIN-resistant) and Canaria Sheep breed (GIN-susceptible) showed no significant between-breed differences after trickle infection with Teladorsagia circumcincta, whereas considerable individual variability was found in both breeds. Next, data from lambs of both breeds were used to explore the relationships between parasitological variables and T. circumcincta-specific IgA levels, local immune cell populations, and abomasal lymph node gene expression to understand the possible mechanisms underlying resistance. Mucosal IgA levels as well as numbers of globular leukocytes and MHC-II+ cells were associated with protection. Analysis of lymph node gene expression revealed the associations between lower parasite numbers and cumulative fecal egg counts and several immune pathways, such as leukocyte cell adhesion, activation and differentiation of T cells, in particular CD4+ and IL-4 production. The data obtained here may inform on the relationship between phenotypic resistance variability and protective responses at the humoral, cellular, and transcriptomic levels, thus contributing to identifying immune responses in young lambs that could be used as markers for selection.

show abstract

Differences in the protection elicited by a recombinant Teladorsagia circumcincta vaccine in weaned lambs of two Canarian sheep breeds

Cited by 7 publications

References 52 publications

Analysis of Protection and Immune Response against Teladorsagia circumcincta in Goats Immunised with Thiol-Binding Proteins from Adult Worms

Analysis of Protection and Immune Response against Teladorsagia circumcincta in Goats Immunised with Thiol-Binding Proteins from Adult Worms

Secretory IgA as Biomarker for Gastrointestinal Nematodes Natural Infection in Different Breed Sheep

Variability in the Response against Teladorsagia circumcincta in Lambs of Two Canarian Sheep Breeds

Contact Info

Product

Resources

About