Differences in responses of grass carp to different types of grass carp reovirus (GCRV) and the mechanism of hemorrhage revealed by transcriptome sequencing

He, Libo; Zhang, Aidi; Pei, Yongyan; Chu, Pengfei; Li, Yongming; Huang, Rong; Liao, Lanjie; Zhang, Zhu; Wang, Yaping

doi:10.1186/s12864-017-3824-1

Cited by 66 publications

(38 citation statements)

References 42 publications

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“…Similar expression pattern was also observed in other pro-apoptotic genes in grass carp [19,32]. Moreover, the dynamic changes of GCRV copy number in infected grass carp was detected in our previously study [33]. Results showed that the relative copy number of GCRV (type II GCRV) was extremely low in 1 and 3 days postinfection, followed by a dramatic increase in 5 days post stimulation and a slight reduction in 7 days post infection.…”

Section: Discussionsupporting

confidence: 82%

Bid-deficient fish delay grass carp reovirus (GCRV) replication and attenuate GCRV-triggered apoptosis

Wang

Luo

et al. 2017

Oncotarget

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Bid, BH3-interacting domain death agonist, is a pro-apoptotic BH3-only member of Bcl-2 family, playing an important role in apoptosis. In the study, Bid genes from grass carp (Ctenopharyngodon idellus) and rare minnow (Gobiocypris rarus), named CiBid and GrBid, were cloned and analyzed. Bid was constitutively expressed in all examined tissues of grass carp, but the expression level varied in different tissues. Following grass carp reovirus (GCRV) stimulation in vivo, Bid and apoptosis related genes Caspase-9 and Caspase-3 was up-regulated significantly at the late stage of infection. Moreover, we generated a Bid-deficient rare minnow (Bid-/-) to investigate the possible role of Bid in GCRV-triggered apoptosis. We found that the survival time of Bid-/- rare minnow after GCRV infection was extended when compared with wild-type fish, the relative copy number of GCRV in Bid-/- rare minnow was lower than that in wild-type fish, and the expression level of Caspase-9 and Caspase-3 in Bid-/- rare minnow were significantly lower than that in the wild-type fish. Collectively, the current data revealed the important role of Bid during virus-induced apoptosis in teleost fish. Our study would provide new insight into understanding the GCRV induced apoptosis and may provide a target gene for virus-resistant breeding in grass carp.

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Section: Discussionsupporting

confidence: 82%

Bid-deficient fish delay grass carp reovirus (GCRV) replication and attenuate GCRV-triggered apoptosis

Wang

Luo

et al. 2017

Oncotarget

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“…Therefore, the six miRNAs that were downregulated may suggest that their target genes upregulated after GCRV infection. Interestingly, we also revealed the overactivity of the complement cascade and immune inflammatory response in grass carp after GCRV infection in our previous study, which is the reason for hemorrhagic symptoms in infected grass carp leading to death [ 40 ]. These results therefore suggest that an mRNA–miRNA–circRNA network is present in grass carp following GCRV infection, providing new insight into the mechanisms underlying GCRV.…”

Section: Discussionmentioning

confidence: 91%

Deep Circular RNA Sequencing Provides Insights into the Mechanism Underlying Grass Carp Reovirus Infection

Zhang

Xiong

et al. 2017

IJMS

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Grass carp hemorrhagic disease, caused by the grass carp reovirus (GCRV), is a major disease that hampers the development of grass carp aquaculture in China. The mechanism underlying GCRV infection is still largely unknown. Circular RNAs (circRNAs) are important regulators involved in various biological processes. In the present study, grass carp were infected with GCRV, and spleen samples were collected at 0 (control), 1, 3, 5, and 7 days post-infection (dpi). Samples were used to construct and sequence circRNA libraries, and a total of 5052 circRNAs were identified before and after GCRV infection, of which 41 exhibited differential expression compared with controls. Many parental genes of the differentially expressed circRNAs are involved in metal ion binding, protein ubiquitination, enzyme activity, and nucleotide binding. Moreover, 72 binding miRNAs were predicted from the differentially expressed circRNAs, of which eight targeted genes were predicted to be involved in immune responses, blood coagulation, hemostasis, and complement and coagulation cascades. Upregulation of these genes may lead to endothelial and blood cell damage and hemorrhagic symptoms. Our results indicate that an mRNA–miRNA–circRNA network may be present in grass carp infected with GCRV, providing new insight into the mechanism underlying grass carp reovirus infection.

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“…However, because of its large with a long reproductive cycle, it is hard to study the antiviral mechanisms in the grass carp in vivo (Lin et al, 2019). CIK cells can be infected by GCRV, and this infection is characterized by the systematic induction of a mild host immune response, involving a variety of classical antiviral signal transduction pathways (Ji et al, 2018;Yu et al, 2017;He et al, 2017). In our previous study, CIK cells were used to study the RNA expression patterns in response to viral infection, including circRNAs, mRNAs, and miRNAs.…”

Section: Discussionmentioning

confidence: 99%

Interleukin-17 suppresses grass carp reovirus infection in Ctenopharyngodon idellus kidney cells by activating NF-κB signaling

Zhang

Liang

et al. 2020

Aquaculture

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A B S T R A C TThe grass carp accounts for a large proportion of aquacultural production in China, but the hemorrhagic disease caused by grass carp reovirus (GCRV) infection often causes huge economic losses to the industry. Interleukin 17 (IL-17) is an important cytokine that plays a critical role in the inflammatory and immune responses. Although IL-17 family members have been extensively studied in mammals, our knowledge of the activity of IL-17 proteins in teleosts in response to viral infection is still limited. In this study, the role of IL-17 in GCRV infection and its mechanism were investigated. The expression levels of IL-17AF1, IL-17AF2, and IL-17AF3 in Ctenopharyngodon idella kidney (CIK) cells gradually increased from 6 h after infection with GCRV. The nuclear translocation of p65, which acts in the NF-κB signaling pathway, was also increased by GCRV infection. The overexpression of IL-17AF1, IL-17AF2, or IL-17AF3 also promoted the nuclear translocation of p65 and the levels of phospho-IκBα in CIK cells, and reduced the expression of the viral structural protein VP7. An NF-κB signal inhibitor abolished the inhibition of GCRV infection by IL-17 proteins. These results suggested that the NF-κB signaling pathway was activated by the overexpression of IL-17 proteins, resulting in the inhibition of viral infection. In conclusion, in this study, we demonstrated that IL-17AF1, IL-17AF2, and IL-17AF3 acted as immune cytokines, exerting an antiviral effect by activating the NF-κB signaling pathway.

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Differences in responses of grass carp to different types of grass carp reovirus (GCRV) and the mechanism of hemorrhage revealed by transcriptome sequencing

Cited by 66 publications

References 42 publications

Bid-deficient fish delay grass carp reovirus (GCRV) replication and attenuate GCRV-triggered apoptosis

Bid-deficient fish delay grass carp reovirus (GCRV) replication and attenuate GCRV-triggered apoptosis

Deep Circular RNA Sequencing Provides Insights into the Mechanism Underlying Grass Carp Reovirus Infection

Interleukin-17 suppresses grass carp reovirus infection in Ctenopharyngodon idellus kidney cells by activating NF-κB signaling

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