Differences in platelet‐rich plasma composition influence bone healing

Abstract

Aim: Platelet-rich plasma (PRP) is an autologous blood-derived material that has been used to enhance bone regeneration. Clinical studies, however, reported inconsistent outcomes. This study aimed to assess the effect of changes in leucocyte and PRP (L-PRP) composition on bone defect healing.Materials and Methods: L-PRPs were prepared using different centrifugation methods and their regenerative potential was assessed in an in-vivo rat model. Bilateral critical-size tibial bone defects were created and filled with single-spin L-PRP, double-spin L-PRP, or filtered L-PRP. Empty defects and defects treated with collagen scaffolds served as controls. Rats were euthanized after 2 weeks, and their tibias were collected and analysed using micro-CT and histology.Results: Double-spin L-PRP contained higher concentrations of platelets than singlespin L-PRP and filtered L-PRP. Filtration of single-spin L-PRP resulted in lower concentrations of minerals and metabolites. In vivo, double-spin L-PRP improved bone

Functionalizing multi-component bioink with platelet-rich plasma for customized in-situ bilayer bioprinting for wound healing

“…The cellular behavior of macrophages is of much concern and a dynamic balance between immune response and regenerated signal during inflammatory phase is the prerequisite for the orderly development of the subsequent proliferation and remodeling phases. It has also been previously reported that activated PLTs could modulate macrophage polarization in vitro and in vivo [ 59 ]. Raw264.7 macrophages were co-cultured with negative control and 3D printed biolayer skin substitutes.…”

Autologous Platelet and Extracellular Vesicle-Rich Plasma as Therapeutic Fluid: A Review

“…The elemental composition of PVRP can be measured by inductively coupled plasma optical emission spectrometry [ 102 ]. Various metabolites in PVRP samples can be determined by ion-parallel liquid chromatography (LC) and mass spectrometry (MS) or gas chromatography (GC) and MS [ 102 ], or by electrochemical sensors [ 103 ]. Hormones can be determined by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-MS /MS) [ 103 , 104 ].…”

The effect of boric acid, calcium fructoborate and potassium boron citrate on bone health in rats

“…Hayvanlar rastgele 4 gruba ayrılmıştır. Laboratuvarda, oda sıcaklığında (25±3 °C), sabit nemli ortamda (55±5 %), gece gündüz periyodunda 3 hafta [14,15] boyunca standart ticari sıçan besini (Bayramoğlu Yem, Erzurum, Türkiye) ile beslenmişlerdir. Besin ve suya ulaşım konusunda herhangi bir kısıtlama yapılmamıştır.…”