Differences in midgut transcriptomes between resistant and susceptible strains of Chilo suppressalis to Cry1C toxin

Chen, Geng; Wang, Yanhui; Liu, Yanmin; Chen, Fajun; Han, Lanzhi

doi:10.1186/s12864-020-07051-6

Cited by 10 publications

(7 citation statements)

References 60 publications

(100 reference statements)

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“…The excessive use of chemical pesticides has led to serious environmental problems, including the ubiquitous resistance of pests and the accumulation of hazardous materials in food and ecosystems. , Thus, botanical insecticides have attracted increasing attention owing to their safer environmental and human health properties . At present, the growth rate of biological pesticides has exceeded that of chemical pesticides, showing positive effects on reducing the use of chemical pesticides .…”

Section: Introductionmentioning

confidence: 99%

Bruceine D Acts as a Potential Insecticide by Antagonizing 20E–EcR/USP Signal Transduction

Chen

Wang

et al. 2023

J. Agric. Food Chem.

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Bruceine D (BD) is an effective insecticidal compound found in the Chinese herb Brucea javanica (L.) Merr. BD inhibits the growth and metamorphosis of Plutella xylostella and Drosophila melanogaster; however, its target protein and the molecular mechanism of insecticidal activity remain unclear. In this study, proteins with high affinity for BD were screened using surface plasmon resonance and high-performance liquid chromatography coupled with matrix-assisted laser desorption/ionizationtime-of-flight mass spectrometry, revealing the ecdysone receptor (EcR) is the main target of BD. In vivo results showed that BD inhibited insect growth and metamorphosis through inhibition of the expression of 20E response genes. In vitro dual luciferase and enhanced green fluorescent protein (EGFP) fluorescence experiments indicated that BD suppressed the transcriptional activation activity of EcR by blocking the ecdysone response element (EcRE)-triggered transcriptional cascade, suggesting that BD inhibits the formation of the 20E−EcR−USP−EcRE complex. Moreover, molecular docking demonstrated that BD bound well to EcR. Elucidating the insecticidal mechanism of BD will be helpful in the development of green pesticides to control pests.

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Section: Introductionmentioning

confidence: 99%

Bruceine D Acts as a Potential Insecticide by Antagonizing 20E–EcR/USP Signal Transduction

Chen

Wang

et al. 2023

J. Agric. Food Chem.

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“…Xenobiotic metabolism is essential in insects as it enables them to neutralize and eliminate toxins that could interfere with vital biochemical processes ( Schuler, 2011 ; Chen G. et al, 2020 ; Palli, 2020 ). Here, multiple P450 genes involved in cytochrome P450-mediated drug and xenobiotic metabolism were upregulated.…”

Section: Discussionmentioning

confidence: 99%

“…The rice stem borer Chilo suppressalis is a highly destructive insect pest in global rice production. It causes significant yield and economic losses and poses a severe threat to international food security ( Chen G. et al, 2020 ; Ma et al, 2020 ). In rice, tps46 is constitutively expressed even in the absence of herbivore attack ( Sun et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Caterpillar-Induced Rice Volatile (E)-β-Farnesene Impairs the Development and Survival of Chilo suppressalis Larvae by Disrupting Insect Hormone Balance

et al. 2022

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Significant research progress has recently been made on establishing the roles of tps46 in rice defense. (E)-β-farnesene (Eβf) is a major product of tps46 activity but its physiological functions and potential mechanisms against Chilo suppressalis have not yet been clarified. In the present study, C. suppressalis larvae were artificially fed a diet containing 0.8 g/kg Eβf and the physiological performance of the larvae was evaluated. In response to Eβf treatment, the average 2nd instar duration significantly increased from 4.78 d to 6.31 d while that of the 3rd instar significantly increased from 5.70 d to 8.00 d compared with the control. There were no significant differences between the control and Eβf-fed 4th and 5th instars in terms of their durations. The mortalities of the 2nd and 3rd Eβf-fed instars were 21.00-fold and 6.39-fold higher, respectively, than that of the control. A comparative transcriptome analysis revealed that multiple differentially expressed genes are involved in insect hormone biosynthesis. An insect hormone assay on the 3rd instars disclosed that Eβf disrupted the balance between the juvenile hormone and ecdysteroid levels. Eβf treatment increased the juvenile hormones titers but not those of the ecdysteroids. The qPCR results were consistent with those of the RNA-Seq. The foregoing findings suggested that Eβf impairs development and survival in C. suppressalis larvae by disrupting their hormone balance. Moreover, Eβf altered the pathways associated with carbohydrate and xenobiotic metabolism as well as those related to cofactors and vitamins in C. suppressalis larvae. The discoveries of this study may contribute to the development and implementation of an integrated control system for C. suppressalis infestations in rice.

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“…When APN1 is downregulated, APN6 is, as a compensatory mechanism, upregulated, which seems to inhibit the toxicity of Cry1Ac and promotes the insect resistance. The reduced expression of receptors, e.g., aminopeptidase-P like proteins, ALPs, and ABC transporters in rice stem borer ( Chilo suppressalis ), is found to promote its resistance to Cry1C, which is also relevant to the transcription levels of enzymes evolved in the hydrolysis and activation of Cry toxin in the mid-intestine ( Chen et al, 2020 ).…”

Section: The Resistance Mechanisms Of Insects Against Cry Toxinsmentioning

confidence: 99%

Which Is Stronger? A Continuing Battle Between Cry Toxins and Insects

Luo

et al. 2021

Front. Microbiol.

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In this article, we review the latest works on the insecticidal mechanisms of Bacillus thuringiensis Cry toxins and the resistance mechanisms of insects against Cry toxins. Currently, there are two models of insecticidal mechanisms for Cry toxins, namely, the sequential binding model and the signaling pathway model. In the sequential binding model, Cry toxins are activated to bind to their cognate receptors in the mid-intestinal epithelial cell membrane, such as the glycophosphatidylinositol (GPI)-anchored aminopeptidases-N (APNs), alkaline phosphatases (ALPs), cadherins, and ABC transporters, to form pores that elicit cell lysis, while in the signaling pathway model, the activated Cry toxins first bind to the cadherin receptor, triggering an extensive cell signaling cascade to induce cell apoptosis. However, these two models cannot seem to fully describe the complexity of the insecticidal process of Cry toxins, and new models are required. Regarding the resistance mechanism against Cry toxins, the main method insects employed is to reduce the effective binding of Cry toxins to their cognate cell membrane receptors by gene mutations, or to reduce the expression levels of the corresponding receptors by trans-regulation. Moreover, the epigenetic mechanisms, host intestinal microbiota, and detoxification enzymes also play significant roles in the insects’ resistance against Cry toxins. Today, high-throughput sequencing technologies like transcriptomics, proteomics, and metagenomics are powerful weapons for studying the insecticidal mechanisms of Cry toxins and the resistance mechanisms of insects. We believe that this review shall shed some light on the interactions between Cry toxins and insects, which can further facilitate the development and utilization of Cry toxins.

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Differences in midgut transcriptomes between resistant and susceptible strains of Chilo suppressalis to Cry1C toxin

Cited by 10 publications

References 60 publications

Bruceine D Acts as a Potential Insecticide by Antagonizing 20E–EcR/USP Signal Transduction

Bruceine D Acts as a Potential Insecticide by Antagonizing 20E–EcR/USP Signal Transduction

Caterpillar-Induced Rice Volatile (E)-β-Farnesene Impairs the Development and Survival of Chilo suppressalis Larvae by Disrupting Insect Hormone Balance

Which Is Stronger? A Continuing Battle Between Cry Toxins and Insects

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