Difference between random and imprinted X inactivation in common voles

Dementyeva, Elena Vladimirovna; Shevchenko, Alexander I.; Anopriyenko, O. V.; Mazurok, Nina A.; Elisaphenko, Eugeny A.; Nesterova, Tatyana B.; Brockdorff, Neil; Zakian, Suren M.

doi:10.1007/s00412-010-0277-6

Cited by 11 publications

(14 citation statements)

References 66 publications

(103 reference statements)

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“…The XX TS cell line (R1) was derived and maintained in the absence of FGF-4 and heparin, a condition that maintains the undifferentiated state of multipotent TS cells in mouse [20]. In vole extraembryonic tissues, the paternal X-chromosome is inactivated [21], [22] and the vole TS cells, like these of mice, demonstrate imprinted XCI and Xist RNA accumulation on Xi (Figure 1A) [19]. The X-chromosome of M. levis is acrocentric and has a giant block of constitutive heterochromatin consisting of two repetitive sequences: MS3 (3265 bp) and MS4 (4088 bp), which contain mobile element fragments [23].…”

Section: Resultsmentioning

confidence: 99%

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Dynamics of the Two Heterochromatin Types during Imprinted X Chromosome Inactivation in Vole Microtus levis

et al. 2014

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In rodent female mammals, there are two forms of X-inactivation – imprinted and random which take place in extraembryonic and embryonic tissues, respectively. The inactive X-chromosome during random X-inactivation was shown to contain two types of facultative heterochromatin that alternate and do not overlap. However, chromatin structure of the inactive X-chromosome during imprinted X-inactivation, especially at early stages, is still not well understood. In this work, we studied chromatin modifications associated with the inactive X-chromosome at different stages of imprinted X-inactivation in a rodent, Microtus levis. It has been found that imprinted X-inactivation in vole occurs in a species-specific manner in two steps. The inactive X-chromosome at early stages of imprinted X-inactivation is characterized by accumulation of H3K9me3, HP1, H4K20me3, and uH2A, resembling to some extent the pattern of repressive chromatin modifications of meiotic sex chromatin. Later, the inactive X-chromosome recruits trimethylated H3K27 and acquires the two types of heterochromatin associated with random X-inactivation.

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Section: Resultsmentioning

confidence: 99%

“…RT-PCR analysis, isolation and analysis of tumors of V1 TS cells were performed as described previously [19], [21].…”

Section: Methodsmentioning

confidence: 99%

Dynamics of the Two Heterochromatin Types during Imprinted X Chromosome Inactivation in Vole Microtus levis

et al. 2014

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“…One such X-linked gene that might escape X inactivation in human chorionic villi is glucose-6-phosphate dehydrogenase (125). In the extraembryonic tissues of common voles (Microtus arvalis), more genes were expressed from the supposedly inactivated X chromosome than detected in somatic tissues (127). Further studies are needed to determine whether other X-associated genes may be incompletely inactivated and thereby account for sexually dimorphic placental responses.…”

Section: Sex Chromosomes and Sexually Dimorphic Effects In The Placentamentioning

confidence: 96%

Sex-Specific Placental Responses in Fetal Development

2015

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The placenta is an ephemeral but critical organ for the survival of all eutherian mammals and marsupials. It is the primary messenger system between the mother and fetus, where communicational signals, nutrients, waste, gases, and extrinsic factors are exchanged. Although the placenta may buffer the fetus from various environmental insults, placental dysfunction might also contribute to detrimental developmental origins of adult health and disease effects. The placenta of one sex over the other might possess greater ability to respond and buffer against environmental insults. Given the potential role of the placenta in effecting the lifetime health of the offspring, it is not surprising that there has been a resurging interest in this organ, including the Human Placental Project launched by the National Institutes of Child Health and Human Development. In this review, we will compare embryological development of the laboratory mouse and human chorioallantoic placentae. Next, evidence that various species, including humans, exhibit normal sex-dependent structural and functional placental differences will be examined followed by how in utero environmental changes (nutritional state, stress, and exposure to environmental chemicals) might interact with fetal sex to affect this organ. Recent data also suggest that paternal state impacts placental function in a sex-dependent manner. The research to date linking placental maladaptive responses and later developmental origins of adult health and disease effects will be explored. Finally, we will focus on how sex chromosomes and epimutations may contribute to sex-dependent differences in placental function, the unanswered questions, and future directions that warrant further consideration.

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“…It has been previously demonstrated that the M. rossiaemeridionalis X chromosome is inactive in Sa006 cell culture and M. arvalis X chromosome is inactive in Sad4 cell culture [26]. Sequencing PCR products from CTCF bound fractions for both Sa006 and Sad4 cell lines detected only the Xist allele corresponded to the active X chromosome (the X chromosome of M. arvalis in Sa006 line and that of M. rossiaemeridionalis in Sad4 line) (Fig.…”

Section: Resultsmentioning

confidence: 82%

A Regulatory Potential of the Xist Gene Promoter in Vole M. rossiaemeridionalis

et al. 2012

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X chromosome inactivation takes place in the early development of female mammals and depends on the Xist gene expression. The mechanisms of Xist expression regulation have not been well understood so far. In this work, we compared Xist promoter region of vole Microtus rossiaemeridionalis and other mammalian species. We observed three conserved regions which were characterized by computational analysis, DNaseI in vitro footprinting, and reporter construct assay. Regulatory factors potentially involved in Xist activation and repression in voles were determined. The role of CpG methylation in vole Xist expression regulation was established. A CTCF binding site was found in the 5′ flanking region of the Xist promoter on the active X chromosome in both males and females. We suggest that CTCF acts as an insulator which defines an inactive Xist domain on the active X chromosome in voles.

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Difference between random and imprinted X inactivation in common voles

Cited by 11 publications

References 66 publications

Dynamics of the Two Heterochromatin Types during Imprinted X Chromosome Inactivation in Vole Microtus levis

Dynamics of the Two Heterochromatin Types during Imprinted X Chromosome Inactivation in Vole Microtus levis

Sex-Specific Placental Responses in Fetal Development

A Regulatory Potential of the Xist Gene Promoter in Vole M. rossiaemeridionalis

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