Dietary schizophyllan reduces mitochondrial damage by activating SIRT3 in mice

Lee, Daeun; Kim, Ye Ram; Kim, Jae Sung; Kim, Dong-Gyu; Kim, Sojin; Kim, Sun Young; Jang, Kiseok; Lee, Jong Dae; Yang, Chul Su

doi:10.1007/s12272-020-01231-4

Cited by 6 publications

(2 citation statements)

References 44 publications

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“…In our study, enforced expression of SIRT3 contributed to enlarged mitochondrial size and enhanced mitochondrial membrane potential both in vitro and in vivo. Lee et al 24 disclosed that schizophyllan supplementation reduced conjugated linoleic acid‐induced adverse dietary effects via SIRT3 –SucD signaling. Our Western blot analysis exhibited that SIRT3 alleviated the mitochondrial dysfunction through promoting the expression of Cyto‐C, SucD, and NADHo in both prostate tissues of BPH rats and PEC.…”

Section: Discussionmentioning

confidence: 99%

SIRT3 affects mitochondrial metabolic reprogramming via the AMPK–PGC‐1α axis in the development of benign prostatic hyperplasia

Wang

Li³

et al. 2021

The Prostate

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BackgroundSirtuin 3 (SIRT3) has been reported to share an association with mitochondrial metabolic reprogramming. However, the molecular mechanism underlying is not well understood, especially in benign prostatic hyperplasia (BPH). Therefore, the purpose of this study was to research whether SIRT3 can affect the progression of BPH via the regulation of mitochondrial metabolic reprogramming.MethodsFollowing the development of a rat model of BPH using testosterone propionate (TP), we extracted prostate tissues from sham‐operated and BPH rats. Subsequently, bioinformatics prediction was used to screen the genes differentially expressed in BPH. To verify the role played by SIRT3 in BPH, we injected AAV9‐SIRT3 into rats, followed by TP treatment. Prostate epithelial cells (PEC) were treated with TP to assess the mitochondrial morphology, mitochondrial membrane potential, and expression of enzymes related to the oxidative phosphorylation pathway after SIRT3 expression alteration. Finally, we examined the expression of AMPK–PGC‐1α pathway in tissues and cells.ResultsSIRT3 was reduced in the prostate tissues of BPH rats. After overexpression of SIRT3, mitochondrial morphology was more stable in prostate tissues of BPH rats and in TP‐treated PEC, with significant increases in mitochondrial membrane potential and in the expression of oxidative phosphorylation‐related enzymes in the cytoplasm. Moreover, SIRT3 significantly activated the AMPK–PGC‐1α signaling pathway, which maintained the stability of mitochondrial membrane potential as well as mitochondrial structure, thus alleviating the symptoms of BPH.ConclusionSIRT3 maintained the stability of mitochondrial membrane potential as well as mitochondrial structure by activating the AMPK–PGC‐1α pathway, thereby alleviating the symptoms of BPH.

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Section: Discussionmentioning

confidence: 99%

SIRT3 affects mitochondrial metabolic reprogramming via the AMPK–PGC‐1α axis in the development of benign prostatic hyperplasia

Wang

Li³

et al. 2021

The Prostate

View full text Add to dashboard Cite

show abstract

“…12,13 Furthermore, a study by D. Lee et al showed that dietary SPG may offer a potential new role in regulating mitochondrial activity to achieve protective immunity in hosts. 14 However, the long fermentation time (>3 d) and limited production rate (1.30 AE 0.02 to 4.20 AE 0.10 mg mL À1 ) of polysaccharides lead to high production costs, limiting the industrial production and commercialization of SPG. 15,16 Radix Puerariae (RP), which is obtained from the root of Pueraria lobate (Willd.)…”

Section: Introductionmentioning

confidence: 99%